Evaluation of cytotoxic effect of photodynamic therapy in combination with electroporation in vitro

Labanauskiene, J.; Gehl, Julie; Didžiapetrienė, Janina

doi:10.1016/j.bioelechem.2006.03.009

Cited by 29 publications

(12 citation statements)

References 10 publications

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“…It is based on the production of reactive oxygen species upon irradiation of the photosensitizer in the presence of oxygen. However, researchers are still developing new sensitizers with potential application in photodynamical treatment Saczko et al 2008;Labanauskiene et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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Two porphyrins, CoTPPS and MnTMPyPCl 5 , were tested for their photodynamic activity and potential novel use in a therapy of human cancers. We investigated an effect of photodynamic reaction (PDR), electroporation (EP) and their combination (electro-photodynamic reaction [EP-PDR]) on human colon adenocarcinoma cell lines (LoVo and resistant to doxorubicin LoVoDX), human breast adenocarcinoma (wild type MCF-7/WT and resistant to doxorubicin MCF-7/DOX), and human melanoma (Me45). The efficiency of macromolecules transport was examined with cytofluorymetry by assessing the degree of propidium iodide (PI) penetration. Additionally, cellular ultrastructure after EP was evaluated. We determined cyto-and photo-cytotoxic effect on the cells viability (MTT assay) after standard PDR and PDR combined with EP. Intracellular distribution and mitochondrial colocalization of both porphyrins was also performed. The experiments proved that both complexes exhibit desirable photodynamic properties on LoVo LoVoDX cells, and EP effectively supports photodynamic method in this type of cancer. The application of EP provided shorter time of incubation (only 10 min) and enhanced effect of applied therapy. The porphyrins did not affect the MCF-7 and Me45 cell lines.

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Section: Introductionmentioning

confidence: 99%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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“…This temporary permeabilization of the plasma membrane allows genes or drugs entering into the cell cytosol. This technique is very attractive in various applications such as electrochemotherapy Mir et al 2006), cutaneous and subcutaneous tumour nodule treatments (Mir et al 1998), evaluation of the cytotoxicity of nonpermeant or poorly permeant anticancer drugs (Labanauskiene et al 2007), gene electrotransfer to various animal tissues (Andre and Mir 2004;Andre et al 2008) and latterly with in vivo tests (Gothelf and Gehl 2010;Heller and Heller 2006).…”

Section: Introductionmentioning

confidence: 99%

A microfluidic biochip for the nanoporation of living cells

Dalmay

Villemejane

Joubert

et al. 2011

Biosensors and Bioelectronics

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This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3 to 10 ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or transgene vectors. This phenomenon permits to envision promising therapies. The presented biochip is composed of thick gold electrodes that are designed to deliver a maximum of energy to the biological medium containing cells. The temporal and spectral distributions of the nsPEF are considered for the design of the chip. In order to validate the fabricated biochip ability to orient the pulse towards the cells flowing within the exposition channels, a frequency analysis is provided. High voltage measurements in the time domain are performed to characterize the amplitude and the shape of the nsPEF within the exposition channels and compared to numerical simulations achieved with a 3D Finite-Difference Time-Domain code. We demonstrate that the biochip is adapted for 3 ns and 10 ns pulses and that the nsPEF are homogenously applied to the biological cells regardless their position along the microfluidic channel. Furthermore, biological tests performed on the developed microfluidic biochip permit to prove its capability to permeabilize living cells with nanopulses. To our knowledge, we report here the first successful use of a microfluidic device optimized for the achievement and real time observation of the nanoporation of living cells.

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“…Table 3 shows that, as with other physical/chemical penetration methods, a great number of studies that use electrochemotherapy are related to PDT and the increase of photosensitizer's permeation through the skin. In each of these studies, electroporation increases drug skin penetration and even drug cytotoxicity (Labanauskiene et al, 2007;Bicek et al, 2007). Combinations of electroporation with other physical methods, such as iontophoresis, often lead to synergistic effects in increasing drug penetration (Johson et al, 2002;Fang et al, 2004).…”

Section: Ruthenium Complexmentioning

confidence: 99%