Evaluation of Cytotoxic, Anti-angiogenic and Antioxidant Properties of Standardized Extracts of Strobilanthes crispus Leaves

Muslim, Nahdzatul Syima; Ng, K. W.; Itam, Afrizal; Nassar, Zeyad D.; Ismail, Zhari; Majid, Amin Malik Shah Abdul

doi:10.3923/ijp.2010.591.599

Cited by 22 publications

(17 citation statements)

References 21 publications

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“…Methanol and water extracts from S. crispa leaves and stems did not cause CNE-1 cell death. This result was consistent with that of a previous study, which demonstrated that the water extract of S. crispa did not exhibit a cytotoxic effect toward any of the cancer cell lines assessed (13). These results suggested that the polar compounds extracted by methanol or water had a less toxic effect against cancer cells.…”

Section: Discussionsupporting

confidence: 93%

“…The water extract of S. crispa has been reported to inhibit the proliferation of retroviruses (11). Previously, S. crispa extract has demonstrated inhibitory properties against breast (12)(13)(14)(15)(16)(17), liver (14,(16)(17)(18)(19)(20), prostate (12) and colon (14) carcinomas. These findings have encouraged further investigation regarding the use of this plant for its anticancer properties.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic and apoptogenic effects of Strobilanthes crispa Blume extracts on nasopharyngeal cancer cells

Koh

Sim

Toh

et al. 2015

Molecular Medicine Reports

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The chemotherapeutic agents used to treat nasopharyngeal cancer (NPC) exhibit low efficacy. Strobilanthes crispa Blume is widely used for its anticancer, diuretic and anti‑diabetic properties. The present study aimed to determine the cytotoxic and apoptogenic effects of S. crispa on CNE‑1 NPC cells. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5 diphenyl tetrazolium bromide assay was used to evaluate the cytotoxic effects of S. crispa against CNE‑1 cells. The rate of apoptosis was determined using propidium iodide staining and caspase assays. Ethyl acetate, hexane and chloroform extracts of S. crispa leaves all exhibited cytotoxic effects on CNE‑1 cells, at a half maximal inhibitory concentration (IC50) of 119, 123.5 and 161.7 µg/ml, respectively. In addition, hexane, chloroform and ethyl acetate extracts of S. crispa stems inhibited CNE‑1 cell proliferation, at a IC50 of 49.4, 148.3 and 163.5 µg/ml, respectively. Flow cytometric analysis revealed an increased proportion of cells in the sub G1 phase and a decreased proportion of cells in the G2/M phase, following treatment with the extracts. However, the extracts did not alter the activities of caspase ‑3/7, ‑8 and ‑9. No cytotoxic effect was observed when the cells were treated with the methanol and water extracts of S. crispa stems and leaves. In conclusion, the S. crispa extracts were cytotoxic against CNE‑1 cells and these extracts were able to induce apoptosis, independent of caspase activation.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Cytotoxic and apoptogenic effects of Strobilanthes crispa Blume extracts on nasopharyngeal cancer cells

Koh

Sim

Toh

et al. 2015

Molecular Medicine Reports

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“…The data from the present study were consistent with those of Mohd-Fadzelly et al [11] who found that the unfermented tea had higher antioxidant activity compared to fermented tea. Another study by Muslim et al [42] who investigated the antioxidant activity of methanolic and aqueous extracts of S. crispus leaves using DPPH free radical scavenging assay showed moderate antioxidant properties compared to controls which were evidenced by the quenching of the DPPH radicals. Gallic acid, quercetin, BHA, and ascorbic acid displayed potent DPPH free radical scavenging activity which produced EC 50 values of 12.6, 15.3, 21.9, and 25.5 µg/ml, respectively.…”

Section: Dpph Free-radical Scavenging Assaymentioning

confidence: 99%

Effect of Oven and Microwave Drying on Polyphenols Content and Antioxidant Capacity of Herbal Tea From Strobilanthes Crispus Leaves

Lasano

Rahmat

Ramli

et al. 2018

Asian J Pharm Clin Res

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Objective: This study aimed to evaluate the effect of oven and microwave drying on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity of unfermented and fermented tea developed from Strobilanthes crispus leaves.Methods: TPC and TFC were estimated using a spectrophotometric method, while antioxidant capacity was determined using ferric reducing antioxidant power assay and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Camellia sinensis (tea plant), that is, used for the production of all varieties of commercial tea and fresh S. crispus leaves were served as controls in this study.Results: The highest antioxidant activity and TPC were observed in S. crispus tea developed from microwave-dried leaves, while the highest TFC was observed in oven-dried tea. Unfermented S. crispus tea showed significantly higher values (p<0.05) for antioxidant activity, TPC, and TFC as compared to fermented S. crispus tea. A strong and moderate correlation was observed between antioxidant activity and TPC as well as TFC values.Conclusion: The present work clearly showed that S. crispus tea developed from microwave-dried leaves able to preserve the polyphenols and hence contribute to excellent antioxidant capacity. Incorporation of unfermented S. crispus tea in the diet can be a good source of natural antioxidant.

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“…Cytisine is a nAChR partial agonist that is currently in clinical use as a smoking cessation aid. Previous studies have conducted radio-ligand displacement experiments to characterize cytisine with regards to its binding affinity for heteromeric nAChRs, using membrane preparations from cells stably expressing human nAChRs (13)(14)(15). Cytisine has also been used for the treatment of central nervous system diseases; it has previously been demonstrated that cytisine may significantly inhibit N-methyl-D aspartate receptor exposure-induced neuronal apoptosis by reversing intracellular Ca 2+ overload and balancing the expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (16).…”

Section: Introductionmentioning

confidence: 99%

Cytisine induces apoptosis of HepG2 cells

Wang

Chen

et al. 2017

Molecular Medicine Reports

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Cytisine is a quinolizidine alkaloid, which has been reported to be among the major bioactive components of Sophora alopecuraides L. Quinolizidine alkaloids have previously been demonstrated to inhibit the proliferation of several types of tumor cells. However, few studies have investigated the effects of cytisine on cancer cells. The present study was performed to further investigate the molecular mechanisms underlying cytisine‑induced apoptosis of HepG2 human hepatocellular carcinoma cells. The results of an MTT assay demonstrated that cytisine inhibited the growth of HepG2 cells in a dose‑dependent manner. In addition, the induction of apoptosis was detected, as determined by morphological observation and flow cytometry. As determined by fluorescence microscopy, apoptotic morphological alterations were detected following cytisine administration. Flow cytometric analyses demonstrated that cytisine induced cytotoxicity through apoptosis‑like mechanisms in HepG2 cells. Furthermore, western blot analysis was performed to investigate the release of cytochrome c (Cyt‑c) and activation of the caspase cascade, and the results indicated that treatment of HepG2 cells with cytisine induced caspase‑dependent apoptosis via the release of Cyt‑c from the mitochondria, upregulation of caspase‑3 and downregulation of pro‑caspase‑3. These results indicated that cytisine may induce apoptosis of HepG2 cells through the mitochondrial pathway.

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Evaluation of Cytotoxic, Anti-angiogenic and Antioxidant Properties of Standardized Extracts of Strobilanthes crispus Leaves

Cited by 22 publications

References 21 publications

Cytotoxic and apoptogenic effects of Strobilanthes crispa Blume extracts on nasopharyngeal cancer cells

Cytotoxic and apoptogenic effects of Strobilanthes crispa Blume extracts on nasopharyngeal cancer cells

Effect of Oven and Microwave Drying on Polyphenols Content and Antioxidant Capacity of Herbal Tea From Strobilanthes Crispus Leaves

Cytisine induces apoptosis of HepG2 cells

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