Evaluation of cytotoxic activities of some medicinal polypore fungi from China

Ren, Guoqing; Liu, X.Y.; Zhu, Hong; Yang, S.Z.; Fu, Cheng

doi:10.1016/j.fitote.2006.05.004

Cited by 49 publications

(29 citation statements)

References 16 publications

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“…The previous results showed that biomass extractability depends on species, strain and solvent [25, 26, 27]. Thus Ren et al [26] demonstrated that yield of Trametes gibbosa basidiocarp extraction in petroleum ether, ethyl acetate and methanol was rather different.…”

Section: Discussionmentioning

confidence: 99%

“…Thus Ren et al [26] demonstrated that yield of Trametes gibbosa basidiocarp extraction in petroleum ether, ethyl acetate and methanol was rather different. Methanol was also good solvent for T .…”

Section: Discussionmentioning

confidence: 99%

“…Numerous searches showed that extracts as well as compounds isolated from various species of the genus Trametes possess significant cytotoxic potential [8, 26, 47, 48]. Abilities of ethanolic extracts of T .…”

Section: Discussionmentioning

confidence: 99%

“…versicolor ethanolic extracts are based on arrest of cell cycle in G 0 /G 1 phase and Harhaji et al [8] reported that fruiting body methanolic extract of this species arrests cycle of melanoma B16 cells in S and G 2 /M phase. Cytotoxic activity of extracts of the studied species can be attributed to proteoglucans, terpenoids and phenol compounds which are soluble in ethanol and that are found in basidiocarp and mycelium extracts of Trametes species [8, 26, 51]. Considering that compounds of Trametes spp.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia

et al. 2018

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In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolor was studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by 1H NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS+ scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia

et al. 2018

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“…The studies focusing on the anticancer effect was mainly done on ethanol, petrol ether, and ethyl acetate extracts. In example, Ren et al showed that petrol ether and ethyl acetate extracts of FPK presented cytotoxicity against Hela and SMMC-7721 cell lines [7]. Hung-Tsung Wu from Taiwan demonstrated in vitro and in vivo the anticancer activity of FPK ethanol-extract [8].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Activity of Fomitopsis Pinicola (Sw. Ex Fr.) Karst Chloroform (Fpkc) Extract Against S180 Tumor Cells

Gao¹,

Wang²,

Wang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Non-toxic fomitopsis is has been traditionally used in folk medicine in many countries for its anti-inflammatory and anti-vascular disease activities. The present study investigates the antitumor effect of Fomitopsis pinicola (Sw. Ex Fr.) Karst chloroform extract (FPKc) on S180 tumor cells in vitro and in vivo and we determined the underlying mechanisms. Methods: HPLC was employed to analyze the constituents of FPKc. In-vitro 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to quantify the growth inhibition of FPKc; Propidium iodide (PI) exclusion assay and scanning electron microscopy (SEM) were used to observe the damage on the cell membrane and the changes of the cell morphology; Staining with Hoechst 33342/propidium iodide (HO/PI) and the application of the Annexin V-FITC/PI analysis permitted to observe the cell death triggered by FPKc; DNA damage and cell cycle arrest were detected by flow cytometry; Rhodamine 123 (RH123) and Cytochrome C were used as dyes to investigate the alterations of the mitochondria. In-vivo tumor inhibition and mice survival time were determined. Results: The results of the HPLC assay indicated that FPKc might contain DA (dehydroeburiconic acid), PA (pachymic acid), and ES (ergosterol), at percentages of 0.25%, 17.8%, and 10.5%, respectively. Concerning the study of the biological function, the results showed that FPKc exhibited preferential and significant suppression of proliferation on specific cell lines including S180, HL-60, U937, K562, SMMC-7721, and Eca-109 cells, which induced plasma membrane and cell morphology damages, triggering S180 tumor-cells late apoptosis and leading to DNA damage and S phase arrest. Mitochondria were suspected to play a vital role in these changes. In vivo data indicated that FPKc inhibited the solid tumor growth and prolonged the survival time of tumor-bearing mice. Moreover, FPKc provoked only little damage on normal cells in vitro and also on normal tissues in vivo. Conclusion: FPKc inhibited S180 tumor cells growth and prolonged the lifespan of mice. In vitro, we found that FPKc induced S180 tumor cells apoptosis and cell cycle arrest, possibly via the mitochondrial pathway.

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Optimization of the Cellulase−Ultrasonic Synergistic Extraction Conditions of Polysaccharides from Lenzites betulina

Guo

Tan

Hui

et al. 2019

Chemistry & Biodiversity

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Lenzites betulina has been recognized as a rich source of chemical components, including polysaccharides, sterides and sugar alcohols. In this study, cellulase−ultrasonic synergistic extraction method was applied to extract polysaccharides from L. betulina, and the response surface methodology was used to optimize the extraction conditions. The eight basic factors affecting extraction yield were evaluated by Plackett−Burman design (PBD). Then, the four important factors significantly affecting the yield of polysaccharides from L. betulina, including enzymolysis temperature, enzymolysis time, ultrasound time and ultrasound temperature, were optimized by Box−Behnken design (BBD). Maximum extraction yield of L. betulina polysaccharides was 13.64±0.09 % at a cellulase dosage of 0.8 %, enzymolysis temperature of 60 °C, enzymolysis time of 180 min, pH of 4.5, liquid‐solid ratio of 45 ml/g, ultrasound power of 300 W, ultrasound time of 20 min and ultrasound temperature of 45 °C. Subsequently, the characteristic structure of crude polysaccharides was determined by FT‐IR. Results indicated that cellulase−ultrasonic synergistic treatment is suitable for L. betulina polysaccharides extraction, and it has good prospect for development and utilization.

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Evaluation of cytotoxic activities of some medicinal polypore fungi from China

Cited by 49 publications

References 16 publications

Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia

Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia

In Vitro and In Vivo Activity of Fomitopsis Pinicola (Sw. Ex Fr.) Karst Chloroform (Fpkc) Extract Against S180 Tumor Cells

Optimization of the Cellulase−Ultrasonic Synergistic Extraction Conditions of Polysaccharides from Lenzites betulina

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