Evaluation of cytostatic drug concentrations in the kidney, bladder wall, and prostate by means of the diffusion chamber technique in dogs

Abstract

The usefulness of a diffusion chamber model for measurement of concentrations of cytostatics in the interstitial fluid of tissues was tested. Chambers (pore size of the membranes: 0.45 mcm) were implanted in the kidney, bladder wall, and prostate of dogs. The concentrations after application of Doxorubicin-hydrochloride (2 mg/kg body wt.) and high doses of Methotrexate (100 mg/kg body wt.) were measured simultaneously and continuously in serum and in the above organs. The investigations showed that knowledge of the plasma level alone does not permit a prediction of the concentrations in the organs to be made. The diffusion chamber technique proved itself to be relatively simple to perform, economical, and it provides reproducible values.