Evaluation of crystal violet impregnated filter paper strips for transport and selective isolation of β-haemolytic streptococci

Malathy, B.; Menon, Thangam

doi:10.1099/jmm.0.47032-0

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“…The remaining 37 were clinical isolates from blood (2), throat swabs (25), skin swabs (5), pus (4) and pleural fluid (1), which were maintained as stock cultures in our laboratory. The swabs were processed by crystal violet filter paper method 9 . Serogrouping was performed using Histrep latex agglutination kit (Hi-media, Mumbai, India) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Genotypic characterization of toxigenic group C and G streptococci isolated in Chennai, South India

Prabu

Menon

2011

Folia Microbiol

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Background & objectives: Increasing resistance to erythromycin has been observed worldwide in group C and group G streptococci (GCS/GGS). The information available from India is scanty. The aim of the study was to identify erythromycin resistant GCS/GGS isolates in Chennai, south India, and to compare erythromycin resistant genotypes with emm types. Methods: One hundred and thirty one GCS/GGS isolates were tested for erythromycin resistance by disc diffusion and agar dilution methods. Erythromycin resistance genotypes [ erm (A), erm (B) and mef (A)] were determined by a multiplex PCR. emm types of erythromycin resistant GCS/GGS isolates was also assessed using emm gene sequencing method. Results: Sixteen of the 131 isolates (12.21%) were resistant to erythromycin. Majority of the isolates were GGS (15/16). Eight of the 16 (50%) were S. dysgalactiae subsps. equisimilis . Twelve isolates (75%) were MLS B phenotype and four (25%) were M phenotype. Of the 12 isolates which exhibited MLS B resistance, seven showed cMLS B phenotype and were positive for erm (B) gene. The remaining five were iMLS B phenotype of which three were positive for erm (A) gene and two for erm (B) gene. erm (A) was common among carriers whereas erm (B) was common among clinical isolates. Interpretation & conclusions: MLS B was the predominant phenotype and erm (B) was the common genotype in the present study. The emm type stC1400.0 was frequently associated with erythromycin resistant GCS/GGS in our study.

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Section: Methodsmentioning

confidence: 99%