Evaluation of cross-protection against three topotypes of serotype O foot-and-mouth disease virus in pigs vaccinated with multi-epitope protein vaccine incorporated with poly(I:C)

Cao, Yimei; Lu, Zengjun; Li, Dong; Fan, Pengcheng; Sun, Pu; Bao, Huang; Fu, Yuanfang; Li, Pinghua; Bai, Xingwen; Chen, Yingli; Xie, Baoxia; Liu, Zaixin

doi:10.1016/j.vetmic.2013.11.023

Cited by 29 publications

(27 citation statements)

References 39 publications

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“…The virus belongs to the genus Aphthovirus and family Picornaviridae with seven serotypes, namely A, O, Asia-1, C, and South African Territory (SAT 1, SAT 2, and SAT 3) having no cross protection. Among these serotypes many subtypes are present (5).…”

Section: Introductionmentioning

confidence: 99%

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Maryam¹,

Rasheed²,

Latif³

et al. 2017

Turk J Vet Anim Sci

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Availability of a rapid and sensitive diagnostic technique is key in successful prevention and control of infectious diseases like foot-and-mouth disease (FMD). Existing conventional diagnostic tests for FMD are laborious and time-consuming with low sensitivity and specificity. Molecular-based techniques are costly and difficult, involving refined apparatus like a thermal cycler. In the present study, the technique of real-time loop-mediated isothermal amplification (RT-LAMP) was standardized for diagnosing FMDV and its serotypes, evaluated using field samples, and compared with the existing real-time PCR in Pakistan. RT-LAMP amplified the target 3D gene using specific primers at 65 °C for 60 min and the VP1 gene using serotype specific primers at 63 °C for 60 min. A total of 38 samples out of 50 were positive by RT-LAMP and identified serotypes were A (n = 15), O (n = 15), and Asia-1 (n = 8). The efficiency of RT-LAMP in this study was highest for serotype Asia-1 (80.9%) followed by serotype A (73.4%) and O (62.35).

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Section: Introductionmentioning

confidence: 99%

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Maryam¹,

Rasheed²,

Latif³

et al. 2017

Turk J Vet Anim Sci

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“…AJ294911) and was characterised by a 93-102 nt deletion within the 3A coding region that is associated with the atypical porcinophilic phenotype of this FMDV lineage [6]. Subsequently, O CATHAY isolates have been confirmed in several Southeast and East Asian countries (including Malaysia, the Philippines, Taiwan, Thailand and Vietnam), although since 1970, the majority of field cases due to this topotype have been reported in Hong Kong SAR and China [7-9]. The O CATHAY FMD outbreak in Taiwan which began during 1997 resulted in the stamping-out of more than 4 million pigs and generated economic losses of over 6 billion US dollars [10].…”

Section: Introductionmentioning

confidence: 99%

Phylodynamic reconstruction of O CATHAY topotype foot-and-mouth disease virus epidemics in the Philippines

Nardo

Knowles

Wadsworth

et al. 2014

Vet Res

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Reconstructing the evolutionary history, demographic signal and dispersal processes from viral genome sequences contributes to our understanding of the epidemiological dynamics underlying epizootic events. In this study, a Bayesian phylogenetic framework was used to explore the phylodynamics and spatio-temporal dispersion of the O CATHAY topotype of foot-and-mouth disease virus (FMDV) that caused epidemics in the Philippines between 1994 and 2005. Sequences of the FMDV genome encoding the VP1 showed that the O CATHAY FMD epizootic in the Philippines resulted from a single introduction and was characterised by three main transmission hubs in Rizal, Bulacan and Manila Provinces. From a wider regional perspective, phylogenetic reconstruction of all available O CATHAY VP1 nucleotide sequences identified three distinct sub-lineages associated with country-based clusters originating in Hong Kong Special Administrative Region (SAR), the Philippines and Taiwan. The root of this phylogenetic tree was located in Hong Kong SAR, representing the most likely source for the introduction of this lineage into the Philippines and Taiwan. The reconstructed O CATHAY phylodynamics revealed three chronologically distinct evolutionary phases, culminating in a reduction in viral diversity over the final 10 years. The analysis suggests that viruses from the O CATHAY topotype have been continually maintained within swine industries close to Hong Kong SAR, following the extinction of virus lineages from the Philippines and the reduced number of FMD cases in Taiwan.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-014-0090-y) contains supplementary material, which is available to authorized users.

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“…This vaccine incorporated linked B cell epitopes (the G–H loop and C terminus of VP1) from four topotypes of serotype O flanked by two universal T cell epitopes. The final product in an oil adjuvant with poly IC protected pigs with 50% protection values of 7–16 against different challenge viruses [(52); Table 2]. …”

Section: Novel Approaches To Vaccines and Vaccinationmentioning

confidence: 99%

Challenges of Generating and Maintaining Protective Vaccine-Induced Immune Responses for Foot-and-Mouth Disease Virus in Pigs

et al. 2016

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Vaccination can play a central role in the control of outbreaks of foot-and-mouth disease (FMD) by reducing both the impact of clinical disease and the extent of virus transmission between susceptible animals. Recent incursions of exotic FMD virus lineages into several East Asian countries have highlighted the difficulties of generating and maintaining an adequate immune response in vaccinated pigs. Factors that impact vaccine performance include (i) the potency, antigenic payload, and formulation of a vaccine; (ii) the antigenic match between the vaccine and the heterologous circulating field strain; and (iii) the regime (timing, frequency, and herd-level coverage) used to administer the vaccine. This review collates data from studies that have evaluated the performance of foot-and-mouth disease virus vaccines at the individual and population level in pigs and identifies research priorities that could provide new insights to improve vaccination in the future.

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Evaluation of cross-protection against three topotypes of serotype O foot-and-mouth disease virus in pigs vaccinated with multi-epitope protein vaccine incorporated with poly(I:C)

Cited by 29 publications

References 39 publications

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Phylodynamic reconstruction of O CATHAY topotype foot-and-mouth disease virus epidemics in the Philippines

Challenges of Generating and Maintaining Protective Vaccine-Induced Immune Responses for Foot-and-Mouth Disease Virus in Pigs

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