Evaluation of Creatine Transport Using Caco‐2 Monolayers as an In Vitro Model for Intestinal Absorption

Dash, Alekha K.; Miller, Donald W.; Huai‐Yan, Han; Carnazzo, Joe; Stout, Jeffrey R.

doi:10.1002/jps.1109

Cited by 21 publications

(11 citation statements)

References 14 publications

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“…Side by side diffusion chambers were used to study the transport across the cell monolayers as previously described. 17 In brief, fresh assay II buffer (3 mL) was placed in the receiver chamber. The donor chamber contained 3 mL of either free PTX solution (25 µg/mL) or gel containing PTX (25 µg/mL).…”

Section: Transport Of Ptx Via In Vitro Cell Culture Modelsmentioning

confidence: 99%

A mucoadhesive in situ gel delivery system for paclitaxel

Jauhari

Dash

2006

AAPS PharmSciTech

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MUC1 gene encodes a transmembrane mucin glycoprotein that is overexpressed in human breast cancer and colon cancer. The objective of this study was to develop an in situ gel delivery system containing paclitaxel (PTX) and mucoadhesives for sustained and targeted delivery of anticancer drugs. The delivery system consisted of chitosan and glyceryl monooleate (GMO) in 0.33M citric acid containing PTX. The in vitro release of PTX from the gel was performed in presence and absence of Tween 80 at drug loads of 0.18%, 0.30%, and 0.54% (wt/wt), in Sorensen's phosphate buffer (pH 7.4) at 37°C. Different mucin-producing cell lines (Calu-39Caco-2) were selected for PTX transport studies. Transport of PTX from solution and gel delivery system was performed in side by side diffusion chambers from apical to basal (A-B) and basal to apical (B-A) directions. In vitro release studies revealed that within 4 hours, only 7.61% ± 0.19%, 12.0% ± 0.98%, 31.7% ± 0.40% of PTX were released from 0.18%, 0.30%, and 0.54% drugloaded gel formulation, respectively, in absence of Tween 80. However, in presence of surfactant (0.05% wt/vol) in the dissolution medium, percentages of PTX released were 28.1% ± 4.35%, 44.2% ± 6.35%, and 97.1% ± 1.22%, respectively. Paclitaxel has shown a polarized transport in all the cell monolayers with B-A transport 2 to 4 times higher than in the A-B direction. The highest mucin-producing cell line (Calu-3) has shown the lowest percentage of PTX transport from gels as compared with Caco-2 cells. Transport of PTX from mucoadhesive gels was shown to be influenced by the mucin-producing capability of cell.

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Section: Transport Of Ptx Via In Vitro Cell Culture Modelsmentioning

confidence: 99%

A mucoadhesive in situ gel delivery system for paclitaxel

Jauhari

Dash

2006

AAPS PharmSciTech

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“…In order to minimize these negative effects, Creatine Hydrocloride (CrHCl) was introduced in the market by Dash et al [7], which is a molecule that is supposed to be 41 times more soluble in water than creatine monohydrate [8]. Moreover, it appears that its permeability in the intestinal tract is also greater than CrM [9].…”

Section: Introductionmentioning

confidence: 99%

Creatine HCl and Creatine Monohydrate Improve Strength but Only Creatine HCl Induced Changes on Body Composition in Recreational Weightlifters

França¹,

Avelar²,

Yoshioka³

et al. 2015

FNS

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“…Regarding creatine, transport was investigated in several cell types; in spite of the increasing physiological relevance of oral creatine supplementation, there is a lack of information regarding intestinal creatine transporter(s), and only few studies have tried to elucidate the mechanisms involved (Peral et al ., ; Persky et al ., ). As reported by other researchers (Dash et al ., ; Vennerstrom & Miller, ), creatine shows poor apical to basolateral permeation in the Caco‐2 cell line model, with a transport of 0.1–14%. This low rate is generally attributed to the low solubility of creatine in water (1.3 g L −1 at 20 °C).…”

Section: Discussionmentioning

confidence: 96%

In vitro intestinal absorption of amino acid mixtures extracted from codfish (Gadus morhua L.) salting wastewater

Ferraro

Jorge

Cruz

et al. 2013

Int J of Food Sci Tech

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This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell's integrity was also examined. Permeation rate (i.e. transport) was ≥95% for all amino acids except for creatine, for which it was 6%. Values for apparent permeability coefficients, P app > 10 À5 cm s À1 , for mixture with isotonic concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute to the fish industry sustainability. Amino acid intestinal absorption V. Ferraro et al. Amino acid intestinal absorption V. Ferraro et al. Amino acid intestinal absorption V. Ferraro et al. Amino acid intestinal absorption V. Ferraro et al. Amino acid intestinal absorption V. Ferraro et al.

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Evaluation of Creatine Transport Using Caco‐2 Monolayers as an In Vitro Model for Intestinal Absorption

Cited by 21 publications

References 14 publications

A mucoadhesive in situ gel delivery system for paclitaxel

A mucoadhesive in situ gel delivery system for paclitaxel

Creatine HCl and Creatine Monohydrate Improve Strength but Only Creatine HCl Induced Changes on Body Composition in Recreational Weightlifters

In vitro intestinal absorption of amino acid mixtures extracted from codfish (Gadus morhua L.) salting wastewater

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