Evaluation of corncob hemicellulosic hydrolysate for xylitol production by adapted strain of Candida tropicalis

Misra, Swati; Raghuwanshi, Shailendra; Saxena, Rajendra Kumar

doi:10.1016/j.carbpol.2012.11.033

Cited by 84 publications

(38 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(Table 4). Cell growth is drastically decreased when concentration of acetate is increased in case of C. parapsilosis [30]. Similar results were observed for D. nepalensis, with an extended lag phase.…”

Section: Xylitol Production From Major Lignocellulosessupporting

confidence: 74%

Bioconversion of Non-Detoxified Hemicellulose Hydrolysates to Xylitol by Halotolerant Yeast Debaryomyces nepalensis NCYC 3413

Paidimuddala¹

2014

J Microb Biochem Technol

View full text Add to dashboard Cite

Lignocellulosic materials are one of the most abundant renewable resources whose exploitation for the production of biochemicals and biofuels is the major challenge in the area of industrial biotechnology due to inhibition of growth and product formation by the toxic compounds released upon their hydrolysis. Indeed the bioprocess that can produce industrial products from hemicellulose hydrolysates in the presence of toxic compounds is economical than the process which involves detoxification. In this study, the ability of halotolerant strain Debaryomyces nepalensis NCYC 3413 to convert non-detoxified xylose enriched hemicellulose hydrolysates from corn cobs, rice straw, sugarcane bagasse and wheat straw to xylitol was evaluated. It was found that this strain has the capability to grow in all hemicellulose hydrolysates and convert xylose to xylitol without detoxification of hydrolysates. The maximum xylitol concentration of 14.6 g L -1 was obtained from corn cobs and wheat straw with productivities of 0.16 and 0.20 g L -1 h -1 respectively at a yield of 0.30 g g -1 . Whereas sugarcane bagasse and rice straw gave xylitol yields of 0.31 and 0.32 g g -1 respectively with 14.2 g L -1 maximum xylitol and productivities were calculated to be 0.20 and 0.15 g L -1 h -1respectively. The presence of high glucose hindered xylitol production by producing ethanol. Based on our findings, we suggest that (i) D. nepalensis is a promising strain for ecofriendly xylitol production as it exhibits broad specificity to lignocellulose substrates, fermentation of mixed sugars and (ii) tolerance towards lignocellulosic inhibitors making the process more economical.

show abstract

Section: Xylitol Production From Major Lignocellulosessupporting

confidence: 74%

Bioconversion of Non-Detoxified Hemicellulose Hydrolysates to Xylitol by Halotolerant Yeast Debaryomyces nepalensis NCYC 3413

Paidimuddala¹

2014

J Microb Biochem Technol

View full text Add to dashboard Cite

show abstract

“…Rafiqul and Sakinah (2015) reported that C. tropicalis using the substrates of Meranti wood sawdust hemicellulosic hydrolysate (MWSHH)-based medium produced XR, activity is 11.16 U/m in 24 h (Table 4). In our study, C. tropicalis strain LY15 was cultivated using corn cob hydrolysate, showing maximum XR assay 62.80 IU/ml (Xylitol 12.08 g/L) within 60 h. Several findings mentioned the uses of agricultural wastes for xylitol production in the presence of XR (Misra et al, 2013). Different agricultural wastes like, corn fiber, corn stover, rice straw, wheat straws, and sugarcane bagasse, comprise hemicellulose (20 to 40%).…”

Section: Discussionmentioning

confidence: 81%

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Barathikannan¹,

Khusro²,

Paul³

2016

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Xylose reductase (XR) is a key enzyme in order to obtain xylitol from xylose. It has vast applications in biotechnology including xylitol production. The present study aimed to estimate and characterize XR from Candida tropicalis strain LY15. C. tropicalis strain LY15 showed xylose utilization ability and xylose reductase activity after 48 h of incubation at pH 6.5, incubation temperature 28°C and rotation speed 140 rpm. It was specific to nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) with an activity of 32.13 IU/ml. Response surface methodology (RSM) was taken into account in order to determine the effect of four main factors, that is, inoculum (1%), hemicellulose waste substrates (HWs) (2%), incubation period (60 h), and RPM (140) on enzyme production. The maximum XR enzyme activity on corn cob substrates (62.80 IU/ml) was found. The xylitol yield (12.08 g/L) attained corn cob media after 60 h of fermentation. Three dimensional response and interaction plot of the quadratic model showed interdependent interaction between the effective variables. Analysis of variance (ANOVA) predicts R 2 value close to 1 which makes the result highly significant (p≤0.0001). These values were higher when compared with the traditional fermentation processes.

show abstract

“…The hemicellulosic fraction of waste materials, such as sugar industry wastes (sugar cane bagasse), provides an important source of xylose that can be converted to xylitol by microbial fermentation (Sarrouh et al 2009;. In this regard, several researchers have explored an alternative route, wherein the selection of naturally occurring xylose-fermenting microorganisms i.e., bacteria, yeasts, and fungi capable of producing xylitol from agro-industrial lignocellulosic residues is emerging as a promising approach (Ji et al 2012;Kamat et al 2012;Misra et al 2013).…”

Section: Chemicals Productionmentioning

confidence: 99%

“…Xylitol has been identified as one of the most valuable chemicals derived from agro-residual lignocellulosic biomass (Kumar et al 2008). Because of its proven marketable applications in food and pharmacological industries, it is an attractive candidate for its low-cost production using lignocellulosic materials (Misra et al 2013). The hemicellulosic fraction of waste materials, such as sugar industry wastes (sugar cane bagasse), provides an important source of xylose that can be converted to xylitol by microbial fermentation (Sarrouh et al 2009;.…”

Section: Chemicals Productionmentioning

confidence: 99%

“…Lignocellulosic materials are renewable resources that can be directly or indirectly used for the production of many useful biological and chemical products (Ghosh and Singh 1993;Moldes et al 2007;Sarrouh et al 2009;Kamat et al 2012;Misra et al 2013). Production of such chemicals could significantly improve the economics of a bio-refinery.…”

Section: Chemicals Productionmentioning

confidence: 99%

See 1 more Smart Citation

Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview

Iqbal¹,

Kyazze²,

Keshavarz³

2013

BioResources

189

View full text Add to dashboard Cite

In view of the worldwide economic and environmental issues associated with the extensive use of petro-chemicals, there has been increasing research interest during the past decade in the value of residual biomass. Because of its renewable nature and abundant availability, residual biomass has attracted considerable attention as an alternate feedstock and potential energy source. To expand the range of natural bio-resources, significant progress related to the lignocellulose biotechnology has been achieved, and researchers have been re-directing their interests to biomass-based fuels, ligninolytic enzymes, chemicals, and biocompatible materials, which can be obtained from a variety of lignocellulosic waste materials. This review article focuses on the potential applications of lignocellulosic materials in biotechnology, including the production of bio-fuels, enzymes, chemicals, the pulp and paper, animal feed, and composites.

show abstract

Evaluation of corncob hemicellulosic hydrolysate for xylitol production by adapted strain of Candida tropicalis

Cited by 84 publications

References 46 publications

Bioconversion of Non-Detoxified Hemicellulose Hydrolysates to Xylitol by Halotolerant Yeast Debaryomyces nepalensis NCYC 3413

Bioconversion of Non-Detoxified Hemicellulose Hydrolysates to Xylitol by Halotolerant Yeast Debaryomyces nepalensis NCYC 3413

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview

Contact Info

Product

Resources

About