Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Abstract

Introduction: Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease. Methodology: The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level. Results: The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively. Conclusion: Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from nontuberculosis mycobacteria (NTM).

Comparison of a Dna Based PCR Approach With Conventional Methods for the Detection of Mycobacterium Tuberculosis in Morocco

“…Moreover, PCR targeting 16S rDNA gene can not differentiate between the closely related species of mycobacteria 10. The hsp65 was also reported to be used as a target for PCR testing to detect MTB DNA in clinical cases, but the necessity of sequencing is still mandatory for the differentiation between the complex MTB and other non tuberculosis mycobacteria 11,29…”

A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis

“…The sensitivity, specificity, positive predictive value, negative predictive value, positive rate, and diagnostic efficiency of our modified acid-fast staining method were evaluated by using the PCR method as the comparison's gold standard (Vishnevskaia et al, 2001;Alli et al, 2011;Chen et al, 2012;Zakham et al, 2012). Briefly, fifty sputum samples from patients with confirmed tuberculosis were analyzed by comparing results from our modified acid-fast staining method and the PCR method .…”

Insight into the diagnosis and management of subclinical genital tuberculosis in women with infertility

“…The presence of PCR inhibitors has been reported in sputum, pus samples, and tissue biopsies. [ 23 ] Shrivastav et al . in 2014[ 24 ] found that PCR-negative samples in endometrial tissue were positive by culture methods and concluded that DNA-PCR done alone is not reliable for TB diagnosis, it should be combined with culture.…”