Evaluation of conidia production and mycelial growth in solid culture media from native strains of entomopathogenic fungi isolated from citrus-growing areas of México

Gandarilla-Pacheco, Fátima Lizeth; Árevalo-Niño, Katiushka; Galán-Wong, Luis J.; Coronado, Carlos F. Sandoval; Quintero-Zapata, Isela

doi:10.5897/ajb12.1658

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Meanwhile, isolates of the genus Isaria showed cumulative growth of 62.67 mm (If1) and 75.42 mm (If2) in the current study, but there are no reports in the literature of similar analyses for other Isaria isolates. The Metarhizium isolate Ma2 showed the highest cumulative radial growth (84.5 mm) among all tested isolates regardless of genus, and this value was superior to those found in the literature (between 57 and 78.1 mm) (Gandarilla-Pacheco et al 2012).…”

Section: Discussioncontrasting

confidence: 49%

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Santos

Freitas

Poderoso

et al. 2018

Florida Entomologist

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Section: Discussioncontrasting

confidence: 49%

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Santos

Freitas

Poderoso

et al. 2018

Florida Entomologist

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“…The physiological characterization of Metarhizium isolates indicated significant differences among isolates in radial growth and conidial germination. This result could be due by the genetic diversity of Metarhizium or at their biochemical activity, because fungal growth can be stimulated by the culture media (Gandarilla-Pacheco et al 2012). In this regard, the present results are similar to those reported by Dimni et al (2004) in M. anisopliae isolates, which achieved a mycelial growth from 2.3 to 3.2 mm day −1 .…”

Section: Discussionsupporting

confidence: 90%

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Basulto

Ruíz-Sánchez

Gómez

et al. 2022

Egypt J Biol Pest Control

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Background The two-spotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is one of the most damaging mites in agriculture. Due to the concern for the intensive use of synthetic acaricides, entomopathogenic fungi represents a feasible alternative to T. urticae management. In the present study, 7 isolates of Metarhizium were characterized physiological and molecularly (based on the ITS1-5.8s-ITS2 rDNA) and evaluated for their acaricidal activity [mortality, mean and 90 lethal concentration (LC50: LC90) and mean and 90 lethal time (LT50: LT90)] against T. urticae under laboratory conditions. Results Sequencing of the ITS1-5.8s-ITS2 rDNA region indicated that the 7 isolates belong to M. anisopliae. The isolates Ma114 (3.7 ± 0.006 mm day−1), Ma109 (3.5 ± 0.009 mm day−1) and Ma106 (3.5 ± 0.006 mm day−1) had the highest radial growth rate and Ma114 (92.2 ± 0.86%) and Ma108 (94.4 ± 1.07%) had the highest germination percentage. All isolates were pathogenic to T. urticae, causing mortality that ranged from 45.3 to 85.3%. The LC50 and LC90 were 1.2 and 2.8, 1.1 and 2.5, and 1.2 and 2.8 × 108 conidia mL−1 for isolates Ma110, Ma109 and Ma106, respectively, while the LT50 and LT90 were 7.7 and 16.5, and 7.2 and 16.1 days for isolates M110 and Ma109, respectively. Conclusion The isolates Ma110 and Ma109 of M. anisopliae were moderately pathogenic and virulent against T. urticae.

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“…Metarhizium anisopliae and Beauveria bassiana are filamentous fungi that belong to the orders Hypocreales, family Clavicipitaceae, and Cordycipitaceae, respectively [23][24][25]. These fungi have a cosmopolitan distribution and have been found in a variety of habitats, including soil, plant debris, and insect cadavers.…”

Section: Introductionmentioning

confidence: 99%

Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production

et al. 2023

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The Green Revolution led to an increased use of synthetic pesticides, causing environmental pollution. As an alternative, biopesticides made from entomopathogenic agents such as fungi have been sought. This study aimed to design and evaluate the performance of a harvester machine for efficiently collecting entomopathogenic spores of Metarhizium anisopliae and Beauveria bassiana grown on rice and corn substrates. The spore yield was estimated, and a spore count and a colony-forming unit (CFU) count were performed. Statistical analysis was conducted to compare the mean values of spores obtained from different combinations of solid substrate and fungi. The Corn-Metarhizium combination produced 34.15 g of spores per kg of substrate and 1.51 × 109 CFUs mL−1. Similarly, the Rice-Metarhizium combination produced 57.35 g per kg and 1.59 × 109 CFUs mL−1. Meanwhile, the Corn-Beauveria combination yielded 35.47 g per kg and 1.00 × 109 CFUs mL−1, while the Rice-Beauveria combination had a yield of 38.26 g per kg and 4.50 × 108 CFUs mL−1. Based on the reported results, the Rice-Metarhizium combination appears to be the most effective, yielding the highest number of harvested spores per kg of substrate. The study estimated a total cost of approximately $409.31 for manufacturing the harvester, considering only the cost of the materials. These results could potentially increase the availability and affordability of entomopathogenic fungi in integrated pest management.

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Evaluation of conidia production and mycelial growth in solid culture media from native strains of entomopathogenic fungi isolated from citrus-growing areas of México

Cited by 5 publications

References 18 publications

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Evaluation of Isolates of Entomopathogenic Fungi in the Genera Metarhizium,Beauveria, and Isaria, and Their Virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production

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