Evaluation of Conditions to Improve Biomass Production by Submerged Culture of Ganoderma sp.

Abstract

In the present investigation, the conditions for in vitro submerged culture of a native strain of Ganoderma sp. were evaluated. Different culture medium ingredients, inoculum concentrations, inoculation methods, configuration, and airflows were evaluated to improve biomass production. The addition of thiamine and olive oil to the culture medium increased biomass production, as well as inoculating 6.6 g/L since there are no significant differences in biomass growth according to inoculum origin (pre-inoculum, discs or with spores). The best configuration of the 3 L stirred tank bioreactor was using three impellers and a porous air diffuser of 0.25 volume per volume per minute (vvm), the dry biomass concentration was 22.6 g/L after 12 days of cultivation at 30 °C, much higher than other investigations. This study provides relevant information for pilot-scale production of this fungus for future secondary metabolites. The culture medium was optimized, and it was defined that the concentration and origin of the inoculum did not influence the growth of Biomass, but the aeration and the configuration of the system allowed the establishment of protocols for the cultivation of Ganoderma sp.

Ganoderma lucidum Mycelia Mass and Bioactive Compounds Production through Grape Pomace and Cheese Whey Valorization

“…Likewise, biomass productivity and conversion yield in shaking conditions were superior to static conditions when fructose and mixed sugars were assessed. The effect of agitation on increased fungal biomass production has been previously demonstrated in several Ganoderma isolates [23][24][25], focusing either solely on optimizing fungal mycelia proliferation or combining it with the production of extracellular or intracellular polysaccharides. Apparently, agitation confers a crucial parameter as it directly relates to oxygen transfer and shear stress, therefore with the size of pellets and secondary metabolites [3].…”

Ganoderma lucidum: Insight into antimicrobial and antioxidant properties with development of secondary metabolites

“…People have been using various methods of cultivation, such as bags filled with wood or straw and wood logs, for decades. For small and pilot plant production, biotechnology has been used for cultivation in bioreactors on solid substrates or with liquid substrates as submerged fermentation [ [173] , [174] , [175] ]. Recent research has paid a lot of attention to improving the production of secondary metabolites [ 176 ].…”