Evaluation of Compounds Extracted from Eight Genera of Wild Mushrooms from Nigeria for Anti-cell Proliferation Activity in Vitro

Abstract

Mushrooms have bioactive compounds that have antimicrobial, anti-cancer and antioxidant activities among other medicinal benefits. In the present study, we examined the anti-cell proliferation activities of mushrooms from eight genera obtained from the wild in Nigeria. Saccharomyces cerevisiae was used as a model organism to screen mushroom extracts for anti-cell proliferation activity. Polyphenols, high molecular weight polysaccharides and low molecular weight compounds from aqueous extracts were obtained from the test mushrooms using methanol and water respectively. The extracts were screened in vitro at different concentrations of extracts with the CyQuant cell proliferation assay. The high molecular weight polysaccharides from tested mushrooms reduced cell proliferation (96.79% inhibition in Ganoderma multipileum Ding Hou to 66.71% inhibition in Coltricia perennis (L.) Murrill at 10.00mg/ml). Percentage inhibition caused by low molecular weight compounds varied from 94.22% (Ganoderma multipileum) to 76.19% (Coltricia perennis) at 10mg/ml. Percentage of inhibition with the polyphenols varied from 94.12% (Microporus xanthopus Fr) Kuntze) to 79.82% (Coltricia perennis) at high doses. High molecular polysaccharides, low molecular weight compounds and polyphenols from mushrooms have anti-cancer properties. The CyQUANT assay proliferation kit was a very efficient tool for screening extracts from wild mushrooms for anti-cell proliferation activities. Medicinal mushrooms in Nigeria show a lot of promise as a reservoir for drug discovery particularly in the area of cancer research.

Antioxidant and Antibacterial Activities of Secondary Metabolites from Microporus xanthopus (Fr.) Kuntze (Polypore) Collected from the Wild in Lagos, Nigeria

“…It is a medicinal mushroom and used as food in Cameroon but not in Nigeria (Kinge et al, 2019). Mushrooms are macro-fungi that possess bioactive compounds (secondary metabolites) that make them effective therapeutic agents in treating infections and various diseases (Niego et al, 2021;Adongbede and Aduralere, 2019). Secondary metabolites are biologically active compounds naturally present in mushroom or plants, they protect their host against pathogens, pest, and help them respond to environmental stress (Iash, 2019).…”