Evaluation of commercial slides for detection of immunoglobulin G againstBartonella henselae by indirect immunofluorescence

Zbinden, Reinhard; Michael, N.; Sekulovski, M.; Graevenitz, A. von; Nadal, David

doi:10.1007/bf01708554

Cited by 33 publications

(32 citation statements)

References 20 publications

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“…The aim of our study was to compare the sensitivity and specificity of our in-house test to that of a recently available commercial test from FT. With CSD patients, our results confirm the previously reported high sensitivity of the FT serological test (29,48,55,65), whereas a lower sensitivity and significantly lower median IgG titers were found with our in-house test. Reactive IgM antibodies were rarely detected for CSD patients by either test, and these antibodies would thus appear to be of little use diagnostically, as previously mentioned (6,27,55,63).…”

Section: Discussionsupporting

confidence: 90%

“…It remains the most frequently used technique, and many laboratories have performed Bartonella serology using tests developed in-house, with reported sensitivities varying considerably, from nearly 100% to less than 30% (54). Commercially prepared antigen slides are now available for B. henselae and B. quintana serology (29,48,55,65), and in this report we compare the sensitivity and specificity of one of these tests with our in-house IFA test, which has been used by our laboratory for 10 years. We compared the abilities of the two tests to detect immunoglobulin G (IgG) and IgM antibodies in serum samples from patients known to have CSD (B. henselae), chronic bacteremia (B. quintana), or endocarditis (B. henselae and B. quintana).…”

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confidence: 99%

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Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M againstBartonella henselaeandBartonella quintana

Maurin

Rolain

Raoult

2002

Clin Vaccine Immunol

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We compared the sensitivities and specificities of indirect fluorescent antibody tests developed in our laboratory and commercially available from Focus Technologies (FT; formerly MRL Diagnostic) for detection of serum antibodies to Bartonella spp. Serum samples tested were from patients with culture-or PCRconfirmed Bartonella quintana or B. henselae infections causing cat scratch disease (CSD), chronic bacteremia, or endocarditis. At a cutoff titer of 64, the FT test had higher sensitivity than our in-house test in detecting anti-B. henselae immunoglobulin G (IgG) antibodies in CSD patients (91.2 versus 52.9%; P < 0.001). The specificity in serum samples from 85 control patients was, however, lower with the FT test (87%) than with the in-house test (98.8%) (P ‫؍‬ 0.002). A cutoff titer of 128 improves the specificity for the FT test but lowers the sensitivity to 85%. For patients infected with B. henselae, our in-house test, but not the FT test, enabled endocarditis to be detected more reliably. With the in-house test, titers of IgG against B. henselae of >1,024 were found only in endocarditis patients and not in CSD patients. With the FT test, 19.1% of CSD patients had titers of IgG against B. henselae of >1,024 (P < 0.001). Our in-house technique also improved detection of anti-B. quintana antibodies in homeless patients with endocarditis. IgG titers of >1,024 were present in 75% of serum samples, but only in 16.7% of serum samples with the FT test (P ‫؍‬ 0.004). Since each test has advantages over the other, the serological diagnosis of Bartonella infections would benefit if both tests were used concurrently.

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Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M againstBartonella henselaeandBartonella quintana

Maurin

Rolain

Raoult

2002

Clin Vaccine Immunol

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“…The sensitivity and specificity of the serological test used in this study were similar to those previously reported (32) and confirm the results of studies showing the high sensitivity of the IFA test for the diagnosis of CSD (44,46). We report a sensitivity of 86.8% at a cutoff titer of Ն64.…”

Section: Discussionsupporting

confidence: 90%

“…The sensitivity of serology varies from one laboratory to another, ranging from near 100% to less than 30% (43). Commercially prepared antigen slides are now available for B. henselae and B. quintana serology (18,20,34,39,44,46). Accurate diagnosis of CSD is necessary because the presentation and course of the disease may resemble more severe diseases such as malignant tumors or mycobacterial infections.…”

mentioning

confidence: 99%

Detection by Immunofluorescence Assay ofBartonella henselaein Lymph Nodes from Patients with Cat Scratch Disease

Rolain

Gouriet

Enea

et al. 2003

Clin Vaccine Immunol

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Laboratory diagnosis of Bartonella henselae infections can be accomplished by serology or PCR assay on biopsy samples. The purpose of our work was to assess immunofluorescence detection (IFD) in lymph node smears using a specific monoclonal antibody directed against B. henselae and a commercial serology assay (IFA) compared with PCR detection. Among 200 lymph nodes examined from immunocompetent patients, 54 were positive for B. henselae by PCR, of which 43 were also positive by IFD. Among the 146 PCR-negative lymph nodes, 11 were positive by IFD. Based on PCR results, the specificity of this new technique was 92.5%, the sensitivity was 79.6%, and the positive predictive value was 79.6%. At a cutoff titer of 64, the sensitivity of the IFA was 86.8% and the specificity was 74.1%. Diagnosis of cat scratch disease (CSD) may be improved, with a specificity of 100%, when the two tests (IFD and IFA) were negative; the sensitivity was 97.4% if one of the two tests was positive. Since PCR-based detection with biopsy samples is available only in reference laboratories, we suggest using IFD coupled with the commercial serology test for the diagnosis of CSD.

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“…Sera from 58 healthy blood donors (Swiss residents) served as control samples as published previously. 10 Positive IgG titres of greater than 1:256 were considered significantly elevated. 10 As routine genetic testing of patients and relatives is currently not yet available, the diagnosis of a familial form of ARVC was essentially based on the exact data of the patients as well as their family history.…”

Section: Methodsmentioning

confidence: 99%

Serological Evidence for the Association of Bartonella henselae Infection with Arrhythmogenic Right Ventricular Cardiomyopathy

Fischer

Loo²,

Shär³

et al. 2008

Clinical Cardiology

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BackgroundArrhythmogenic right ventricular cardiomyopathy (ARVC) is an important cause of sudden death in young adults. On the basis of histopathological findings its pathogenesis may involve both a genetic origin and an inflammatory process. Bartonella henselae may cause endomyocarditis and was detected in myocardium from a young male who succumbed to sudden cardiac death.HypothesisWe hypothesized that chronic infection with Bartonella henselae could contribute to the pathogenesis of ARVC.MethodsWe investigated sera from 49 patients with ARVC for IgG antibodies to Bartonella henselae. In this study, 58 Swiss blood donors tested by the same method served as controls.ResultsSix patients with ARVC (12%) had positive (>1:256) IgG titres in the immunofluorescence test with Bartonella henselae. In contrast, only 1 elevated titre was found in 58 controls (p ≤ 0.05). Interestingly, all patients with increased titres had no familial occurrence of ARVC.ConclusionsFurther studies in larger patient cohorts seem justified to investigate a possible causal link between chronic Bartonella henselae and ARVC, in particular its sporadic (nonfamilial) form. Copyright © 2008 Wiley Periodicals, Inc.

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Evaluation of commercial slides for detection of immunoglobulin G againstBartonella henselae by indirect immunofluorescence

Cited by 33 publications

References 20 publications

Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M againstBartonella henselaeandBartonella quintana

Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M againstBartonella henselaeandBartonella quintana

Detection by Immunofluorescence Assay ofBartonella henselaein Lymph Nodes from Patients with Cat Scratch Disease

Serological Evidence for the Association of Bartonella henselae Infection with Arrhythmogenic Right Ventricular Cardiomyopathy

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