Evaluation of commercial latex agglutination reagents for grouping streptococci

Facklam, Richardr .; Cooksey, Robert C.; Wortham, E C

doi:10.1128/jcm.10.5.641-646.1979

Cited by 33 publications

(15 citation statements)

References 13 publications

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“…The Phadebact Streptococcus Test and the Streptex Test kits provide the clinical laboratory 13 with acceptable alternatives to classical serological methods for grouping streptococci (7,(19)(20)(21)(22). Tests may be performed on colonies taken from primary plates or on broth subcultures.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, it is time consuming and requires technical skill and quality antisera for accurate results. Presumptive identification of streptococci as group A by bacitracin susceptibility testing, as group B by hippurate hydrolysis or by the CAMP test, or as group D by esculin hydrolysis is simple to perform (7). However, results are not always reliable, since false-posi-tive or false-negative reactions occur.…”

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confidence: 99%

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Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures

Wellstood¹

1982

J Clin Microbiol

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The Phadebact Streptococcus Test and the Streptex Test kits were evaluated for grouping streptococci directly from blood cultures. Pellets of bacteria obtained from centrifuged samples of positive blood cultures were inoculated into Todd-Hewitt broth for 2-and 4-h Phadebact tests and into pronase for Streptex tests. Hemolysis was determined after pipetting a portion of each pellet into cuts made in blood agar plates incubated anaerobically for 2 to 6 h. Serological groups were also determined from colonies of the 137 strains of streptococci used in the study by the Lancefield precipitin method. Of the 126 strains tested by the 4-h Phadebact method, 120 (95.2%) agreed with Lancefield groupings, and 133 (97.1%) of the 137 strains tested by Streptex were in agreement. In contrast, only 31 of 55 strains (56.4%) were correctly identified by the 2-h Phadebact method. Misidentifications were related to multiple agglutinations and weak agglutinations in homologous antisera. Group A isolates were most frequently misidentified by all of the test methods. Hemolysis was determined within 4 h for 92.7% of the isolates and within 6 h for the remaining strains. Although the 4-h Phadebact procedure and the Streptex procedure were comparable in overall accuracy, cost, and technologist time, Streptex was the method of choice for direct groupings. Results were available within 75 min for the Streptex procedure compared with 4 h for the Phadebact method. Because few cross-reactions occurred, agglutination responses were clearer and easier to interpret. Results from 2-h Phadebact tests were not satisfactory, and this method is not recommended.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures

Wellstood¹

1982

J Clin Microbiol

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“…Ruoff et al (102) used concentration methods on strains which did not give a positive test and succeeded in showing a D antigen, which was confirmed by gel diffusion. Serologic tests which are technically easier, such as latex bead agglutination, often fail to detect the D antigen in S. bovis (20,46,102).…”

Section: Identificationmentioning

confidence: 99%

Classification and identification of the viridans streptococci

1989

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This review traces the history of the human, nonhemolytic, or viridans, streptococci and describes improvements in their taxonomy wrought by study of their biochemical profiles and analysis of their nucleic acids. The goal was to define species on the basis of genetic relationships and to describe these species by their phenotypic characteristics so that they can be easily identified. This method has resulted in the division of some species. Streptococcus mutans has been divided into four species, two of which are common in humans. Three more mutans group species are indigenous to animals. Conversely, S. constellatus, S. intermedius, and "S. milleri" have been combined under S. anginosus. S. mitis (or "S. mitior") can be well-defined and includes S. sanguis II. There is genetic heterogeneity within S. sanguis, but the species is usually easy to identify. There is also some heterogeneity in S. bovis, but most human isolates are genetically related. Discussions of the taxonomy of these species are accompanied by descriptions of the characteristics by which these streptococci can be identified. Among these species are potential pathogens which should be suspected in cases of endocarditis and purulent infections of liver, brain, and other tissues.

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“…The Streptex latex agglutination procedure correctly identified 91.7% of the strains tested. In contrast, Facklam et al reported 97% accuracy with the Streptex procedure and cautioned that an inoculum heavier than that recommended by the manufacturer was desirable, especially when testing small colonies (5). Similarly, Slifkin reported 99.5 % accuracy when an overnight broth culture was used as a source of antigen (11).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the API 20S Streptococcus identification system with an immunorheophoresis procedure and two commercial latex agglutination tests for identifying beta-hemolytic streptococci

Keville¹,

Doern²

1982

J Clin Microbiol

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The API 20S Streptococcus identification system and a new immunorheophoresis procedure were evaluated as means for determining the Lancefield serogroup of beta-hemolytic streptococci recovered from human clinical specimens. The serogroup of 96 strains was determined by these methods and by two commercially available latex agglutination tests, Streptex and SeroSTAT. The results of all four procedures were compared with the results of a classical precipitin test. The API 20S system correctly categorized 92.7% of the isolates; 94.8% were correctly identified by the immunorheophoresis procedure. The latex agglutination procedures were of comparable accuracy, yielding correct identifications with approximately 92% of the strains tested.

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Evaluation of commercial latex agglutination reagents for grouping streptococci

Cited by 33 publications

References 13 publications

Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures

Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures

Classification and identification of the viridans streptococci

Comparison of the API 20S Streptococcus identification system with an immunorheophoresis procedure and two commercial latex agglutination tests for identifying beta-hemolytic streptococci

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