Evaluation of commercial ELISA kits for diagnosis of brucellosis in cattle and buffaloes in different epidemiological scenarios

Sarangi, Laxmi Narayan; Surendra, Kota Sri Naga Leela; Rana, Samir Kumar; Naveena, Thodangala; Prasad, Amitesh; Ponnanna, Nadikerianda Muthappa; Sharma, Girish Kumar

doi:10.1016/j.mimet.2022.106449

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…There are reports in the literature of assays developed for livestock being used for antibody detection in wildlife. These include studies in which assays developed for horses were used for zebra ( 51 ), assays developed for domestic dogs were used for African wild dogs ( 52 ), assays developed for domestic cats were used for lions ( 53 ), and assays developed for domestic cattle were used for African buffalo ( 54 ). However, in this study, we report a significant variation between domestic cattle and African buffalo within the Bovini tribe.…”

Section: Discussionmentioning

confidence: 99%

Development of conjugated secondary antibodies for wildlife disease surveillance

et al. 2023

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Disease monitoring in free-ranging wildlife is a challenge and often relies on passive surveillance. Alternatively, proactive surveillance that relies on the detection of specific antibodies could give more reliable and timely insight into disease presence and prevalence in a population, especially if the evidence of disease occurs below detection thresholds for passive surveillance. Primary binding assays, like the indirect ELISA for antibody detection in wildlife, are hampered by a lack of species-specific conjugates. In this study, we developed anti-kudu (Tragelaphus strepsiceros) and anti-impala (Aepyceros melampus) immunoglobulin-specific conjugates in chickens and compared them to the binding of commercially available protein-G and protein-AG conjugates, using an ELISA-based avidity index. The conjugates were evaluated for cross-reaction with sera from other wild herbivores to assess future use in ELISAs. The developed conjugates had a high avidity of >70% against kudu and impala sera. The commercial conjugates (protein-G and protein-AG) had significantly low relative avidity (<20%) against these species. Eighteen other wildlife species demonstrated cross-reactivity with a mean relative avidity of >50% with the impala and kudu conjugates and <40% with the commercial conjugates. These results demonstrate that species-specific conjugates are important tools for the development and validation of immunoassays in wildlife and for the surveillance of zoonotic agents along the livestock-wildlife-human interface.

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Section: Discussionmentioning

confidence: 99%

Development of conjugated secondary antibodies for wildlife disease surveillance

et al. 2023

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Nosological Features of Infectious Abortions in Cows

Radzyhovskyi,

Sachuk,

Koshevoy

et al. 2024

SCIVP

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Providing the country's population with high-quality products is a priority task of modern animal husbandry. However, there are a number of problems in the industry that prevent obtaining quality products of animal origin in the required volume. The intensification of dairy farming and the increase in milk productivity of cows is often accompanied by a metabolic disorder, which reduces the body's resistance, contributing to the development of immune-deficiencies, which increase the susceptibility of animals to infectious diseases, some of which have an abortive form of manifestation and, accordingly, the consequence is a lack of offspring and potential income due to abortions. Abortion in cows should be considered not only as a gynecological problem, but also as an economic one, because animal husbandry accounts for more than 38% in the structure of gross agricultural products of the country and the lack of keeping of young animals significantly affects the development of the livestock industry. This publication is devoted to the results of the nosological profile of infectious abortions in cows on the territory of Ukraine, among which 64% of cases are infectious agents - 41.4% of bacterial origin and 22.6% of viral origin. The most frequent factors of bacterial etiology of abortions in cows are leptospira, coxiella, salmonella, mycoplasma, campylobacter, chlamydia, toxoplasma and listeria, and the most common viral ones are flavo-virus, gamma-herpesvirus and alpha-herpesvirus.

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Evaluation of commercial ELISA kits for diagnosis of brucellosis in cattle and buffaloes in different epidemiological scenarios

Cited by 2 publications

References 14 publications

Development of conjugated secondary antibodies for wildlife disease surveillance

Development of conjugated secondary antibodies for wildlife disease surveillance

Nosological Features of Infectious Abortions in Cows

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