Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens

Matsiota-Bernard, P.; Pitsouni, E.; Legakis, N. J.; Nauciel, C

doi:10.1128/jcm.32.6.1503-1505.1994

Cited by 58 publications

(24 citation statements)

References 12 publications

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“…9 PCR has repeatedly been shown to have sensitivity equal to or greater than culture when testing lower respiratory specimens. [67][68][69][70][71][72][73] Legionella DNA can also be detected in nonrespiratory specimens, such as urine, serum, and peripheral leukocytes, 9 although testing these specimen types is not well established. Abbreviations: DFA, direct fluorescence assay; ND, not determined; TCID 50 , tissue culture infective dose needed to produce 50% change.…”

Section: Naats For Specific Respiratory Pathogensmentioning

confidence: 99%

Emerging Advances in Rapid Diagnostics of Respiratory Infections

Murdoch

Jennings

Bhat³

et al. 2010

Infectious Disease Clinics of North America

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Recent developments in rapid diagnostics for respiratory infections have mostly occurred in the areas of antigen and nucleic acid detection. Nucleic acid amplification tests have improved the ability to identify respiratory viruses in clinical specimens and have played pivotal roles in the rapid characterization of new viral pathogens. Antigen-detection assays in immunochromatographic or similar formats are most easily developed as near-patient tests, although they have been developed commercially only for a limited range of respiratory pathogens. New approaches for respiratory pathogen detection are needed, and breath analysis is an exciting area with enormous potential.

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Section: Naats For Specific Respiratory Pathogensmentioning

confidence: 99%

Emerging Advances in Rapid Diagnostics of Respiratory Infections

Murdoch

Jennings

Bhat³

et al. 2010

Infectious Disease Clinics of North America

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“…A commercial kit from Perkin-Elmer Cetus (EnviroAmp') for the detection of legionellae in environmental specimens was introduced in 1992. Reverse dot-blotting of PCR products is used to detect legionellae at the genus level with a 5s rDNA amplicon and the species L. pneumophilu with a separate mip gene amplicon [8].…”

Section: Introductionmentioning

confidence: 99%

Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting

Robinson

Heidrich

Tiecke

et al. 1996

FEMS Microbiology Letters

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Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system.

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“…In epidemiological or clinical scenarios, a PCR test, due to its high sensitivity, may be the only positive test for a patient with Legionnaires' disease. On the basis of the published sequence of the 5S rRNA gene of L. pneumophila (7), a 108-bp region has been used as a target for PCR detection of all species of Legionella in clinical and environmental samples (3,8). The genes that code rRNA are highly conserved and have specific sequences related to the phylogenetic status of bacteria (1).…”

mentioning

confidence: 99%

Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences

et al. 1997

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Seventeen different species of Legionella, 12 serogroups of Legionella pneumophila, and 2 Legionella-like amoebal pathogens (LLAP1 and Sarcobium lyticum) were examined by heteroduplex analysis of PCR products of the 5S rRNA gene. Eight different banding patterns were identified, indicating that heteroduplex analysis of this gene can be used to classify these bacteria according to base substitutions between species. This classification may have future applications in clinical and epidemiological studies.

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Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens

Cited by 58 publications

References 12 publications

Emerging Advances in Rapid Diagnostics of Respiratory Infections

Emerging Advances in Rapid Diagnostics of Respiratory Infections

Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting

Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences

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