Evaluation of collagenase, elastase and tyrosinase inhibitory activities of Cotinus coggygria Scop. through in vitro and in silico approaches

Deniz, Fatma Sezer Şenol; Salmas, Ramin Ekhteiari; Emerce, Esra; Cankaya, Iffet Irem Tatli; Yusufoğlu, Hasan Soliman; Orhan, Ilkay Erdogan

doi:10.1016/j.sajb.2020.05.017

Cited by 22 publications

(17 citation statements)

References 41 publications

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“…A particularly affected target in this theme is the extracellular matrix (ECM), a three-dimensional network of elastin and collagen fibers surrounded by the ground substances, such as hyaluronic acid (HA), that act together to maintain skin filling, elasticity, and flexibility [ 3 ]. Under a framework of oxidative-stress, elastin and collagen fibers, responsible for the elasticity and resistance of the skin, may lose their structure [ 4 ]. This phenomenon, partly due to the deregulated activity of the matrix metalloproteinases (MMPs), such as elastase, collagenase, and hyaluronidase, is at the base of premature skin aging associated with external factors [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

et al. 2022

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The loss of density and elasticity, the appearance of wrinkles and hyperpigmentation are among the first noticeable signs of skin aging. Beyond UV radiation and oxidative stress, matrix metalloproteinases (MMPs) assume a preponderant role in the process, since their deregulation results in the degradation of most extracellular matrix components. In this survey, four cyanobacteria strains were explored for their capacity to produce secondary metabolites with biotechnological potential for use in anti-aging formulations. Leptolyngbya boryana LEGE 15486 and Cephalothrix lacustris LEGE 15493 from freshwater ecosystems, and Leptolyngbya cf. ectocarpi LEGE 11479 and Nodosilinea nodulosa LEGE 06104 from marine habitats were sequentially extracted with acetone and water, and extracts were analyzed for their toxicity in cell lines with key roles in the skin context (HaCAT, 3T3L1, and hCMEC). The non-toxic extracts were chemically characterized in terms of proteins, carotenoids, phenols, and chlorophyll a, and their anti-aging potential was explored through their ability to scavenge the physiological free radical superoxide anion radical (O2•−), to reduce the activity of the MMPs elastase and hyaluronidase, to inhibit tyrosinase and thus avoid melanin production, and to block UV-B radiation (sun protection factor, SPF). Leptolyngbya species stood out for anti-aging purposes: L. boryana LEGE 15486 presented a remarkable SPF of 19 (at 200 µg/mL), being among the best species regarding O2•− scavenging, (IC50 = 99.50 µg/mL) and also being able to inhibit tyrosinase (IC25 = 784 µg/mL), proving to be promising against UV-induced skin-aging; L. ectocarpi LEGE 11479 was more efficient in inhibiting MMPs (hyaluronidase, IC50 = 863 µg/mL; elastase, IC50 = 391 µg/mL), thus being the choice to retard dermal density loss. Principal component analysis (PCA) of the data allowed the grouping of extracts into three groups, according to their chemical composition; the correlation of carotenoids and chlorophyll a with MMPs activity (p < 0.01), O2•− scavenging with phenolic compounds (p < 0.01), and phycocyanin and allophycocyanin with SPF, pointing to these compounds in particular as responsible for UV-B blockage. This original survey explores, for the first time, the biotechnological potential of these cyanobacteria strains in the field of skin aging, demonstrating the promising, innovative, and multifactorial nature of these microorganisms.

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Section: Introductionmentioning

confidence: 99%

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

et al. 2022

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“…As example, the down‐regulation of tyrosinase activity is the commonly reported approach for the treatment of melanogenesis, bearing in mind that it catalyzes the first and only rate‐limiting steps of melanin synthesis [56] . Elastin, an important protein being the principal component of the connective tissue, is responsible for the elasticity and resistance of the skin [57] . Hydrolysis of elastin through elastase result in a loss of skin elasticity and damage, and in turn provide a predisposition wrinkle emergence.…”

Section: Discussionmentioning

confidence: 99%

A Prospective of Multiple Biopharmaceutical Activities of Procyanidins‐Rich Uapaca togoensis Pax Extracts: HPLC‐ESI‐TOF‐MS Coupled with Bioinformatics Analysis

Sinan

Aktümsek

Cádiz-Gurrea

et al. 2021

Chemistry & Biodiversity

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The article reports the chemical composition, antioxidant, six key enzymes inhibitory and antimicrobial activities of two solvent extracts (water and methanol) of leaves and stem bark of Uapaca togoensis. For chemical composition, methanol extract of stem bark exhibited significant higher total phenolic (129.86 mg GAE/g) and flavanol (10.44 mg CE/g) contents. Methanol extract of leaves and water extract of stem bark showed high flavonoids (20.94 mg RE/g) and phenolic acid (90.40 mg CAE/g) content, respectively. In addition, HPLC‐ESI‐TOF‐MS analysis revealed that U. togoensis was rich in procyanidins. The methanol and water extracts of stem bark had overall superior antioxidant activity; however, only methanol extract of stem bark showed higher inhibition of cholinesterase (AChE: 2.57 mg GALAE/g; BChE: 4.69 mg GALAE/g), tyrosinase (69.53 mg KAE/g) and elastase (2.73 mmol CE/g). Potent metal chelating ability was showed by water extract of leaves (18.94 mg EDTAE/g), higher inhibition of amylase was detected for water extracts of leaves (0.94 mmol ACAE/g) and stem bark (0.92 mmol ACAE/g). The tested extracts have shown wide‐spectrum antibacterial properties and these effects have shown to be more effective against Aspergillus ochraceus, Penicillium funiculosum, Trichoderma viride, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The results revealed that the antioxidant, enzyme inhibitory and antimicrobial activities depended on the extraction solvents and the parts of plant. Bioinformatics analysis on the 17 major compounds showed modulation of pathway associated with cancer. In brief, U. togoensis might be valuable as potential source of natural agents for therapeutic application.

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“…Matrix metalloproteinases (MMPs) are a family of extracellular zinc-dependent enzymes, which main function is to remodel and degrade the ECM 30 . Collagen and elastin are primary proteins of the ECM, responsible for resistance and elasticity of the skin 32 . Therefore, any alterations in collagen and elastin induced by MMPs, will contribute to the loss of dermal structure, resulting in its damage 33 .…”

Section: Metalloproteinasesmentioning

confidence: 99%

Cyanobacteria and microalgae bioactive compounds in skin-ageing: potential to restore extracellular matrix filling and overcome hyperpigmentation

Favas

Morone

Martíns

et al. 2021

Journal of Enzyme Inhibition and Medicinal Chemistry

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As the largest organ in human body, skin acts as a physicochemical barrier, offering protection against harmful environmental stressors, such as chemicals, pathogens, temperature and radiation. Nonetheless, skins prominence goes further, with a significant psychosocial role in an increasingly ageing population. Prompted by consumers' concern regarding skincare, cosmetic industry has been developing new formulas capable of lessening the most visible signs of ageing, including reduction in skin density and elasticity, wrinkling and hyperpigmentation. Allied to skincare is the rising importance set on natural products, sustainably obtained from less environmental impacting methods. Cyanobacteria and microalgae are adding importance in this field, given their ability to biosynthesize secondary metabolites with anti-ageing potential. In this review, we present an overview on the potential of cyanobacteria and microalgae compounds to overcome skin-ageing, essentially by exploring their effects on the metalloproteinases collagenase, elastase, gelatinase and hyaluronidase, and in other enzymes involved in the pigmentation process.

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Evaluation of collagenase, elastase and tyrosinase inhibitory activities of Cotinus coggygria Scop. through in vitro and in silico approaches

Cited by 22 publications

References 41 publications

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

A Prospective of Multiple Biopharmaceutical Activities of Procyanidins‐Rich Uapaca togoensis Pax Extracts: HPLC‐ESI‐TOF‐MS Coupled with Bioinformatics Analysis

Cyanobacteria and microalgae bioactive compounds in skin-ageing: potential to restore extracellular matrix filling and overcome hyperpigmentation

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