Evaluation of Collagen Gel-Associated Human Nasal Septum-Derived Chondrocytes As a Clinically Applicable Injectable Therapeutic Agent for Cartilage Repair

Lim, Mi Hyun; Jeun, Jung Ho; Kim, Do Hyun; Park, Sun Hwa; Kim, Seok Jung; Lee, Weon Sun; Hwang, Se Hwan; Lim, Jung Yeon; Kim, Sung Won

doi:10.1007/s13770-020-00261-9

Cited by 7 publications

(7 citation statements)

References 40 publications

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“…On the basis of our findings, we suggest that simultaneous application of both cells and growth factors, which play pivotal and complementary roles during tissue regeneration, are likely to be essential for effective healing of tissues with avascular structures ( e.g. , cartilage , ) and wounds with weakened/imperfect blood vessels ( e.g. , diabetic foot ulcer).…”

Section: Resultsmentioning

confidence: 72%

“…This is probably due to the avascular structure of IVD resulting in highly restricted supply of bioactive molecules to induce cell differentiation (as seen with the BMSC group) and limited recruitment of cells in response to continuously released TGF-β3 (as seen with the TGF group). On the basis of our findings, we suggest that simultaneous application of both cells and growth factors, which play pivotal and complementary roles during tissue regeneration, are likely to be essential for effective healing of tissues with avascular structures (e.g., cartilage 90,91 ) and wounds with weakened/imperfect blood vessels (e.g., diabetic foot ulcer). Because of their unique structure and biochemically benign properties, LSS particles can provide both cell adhesiveness and an ability to provide continuous release of growth factors.…”

Section: ■ Experimental Sectionmentioning

confidence: 73%

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Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

et al. 2020

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Although biological therapies based on growth factors and transplanted cells have demonstrated some positive outcomes for intervertebral disc (IVD) regeneration, repeated injection of growth factors and cell leakage from the injection site remain considerable challenges for human therapeutic use. Herein, we prepare human bone marrow-derived mesenchymal stem cells (hBMSCs) and transforming growth factor-β3 (TGF-β3)-loaded porous particles with a unique leaf-stack structural morphology (LSS particles) as a combination bioactive delivery matrix for degenerated IVD. The LSS particles are fabricated with clinically acceptable biomaterials (polycaprolactone and tetraglycol) and procedures (simple heating and cooling). The LSS particles allow sustained release of TGF-β3 for 18 days and stable cell adhesiveness without additional modifications of the particles. On the basis of in vitro and in vivo studies, it was observed that the hBMSCs/TGF-β3-loaded LSS particles can provide a suitable milieu for chondrogenic differentiation of hBMSCs and effectively induce IVD regeneration in a beagle dog model. Thus, therapeutically loaded LSS particles offer the promise of an effective bioactive delivery system for regeneration of various tissues including IVD.

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Section: Resultsmentioning

confidence: 72%

Section: ■ Experimental Sectionmentioning

confidence: 73%

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

et al. 2020

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“… 188 And the relatively high concentration (2–10 μM) of Mg ions facilitate chondrogenesis and osteogenesis instead of adipogenesis of BMSCs and tendon-derived MSCs under induction conditions respectively ex vivo. 189 …”

Section: Articulate Cartilage Defects Therapeutic Strategiesmentioning

confidence: 99%

Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

Guo

Ling

et al. 2023

J Tissue Eng

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Articular cartilage (AC), a bone-to-bone protective device made of up to 80% water and populated by only one cell type (i.e. chondrocyte), has limited capacity for regeneration and self-repair after being damaged because of its low cell density, alymphatic and avascular nature. Resulting repair of cartilage defects, such as osteoarthritis (OA), is highly challenging in clinical treatment. Fortunately, the development of tissue engineering provides a promising method for growing cells in cartilage regeneration and repair by using hydrogels or the porous scaffolds. In this paper, we review the therapeutic strategies for AC defects, including current treatment methods, engineering/regenerative strategies, recent advances in biomaterials, and present emphasize on the perspectives of gene regulation and therapy of noncoding RNAs (ncRNAs), such as circular RNA (circRNA) and microRNA (miRNA).

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“…35 To overcome the limited source of autologous chondrocytes, Lim et al added human nasal septum-derived chondrocytes (hNCs) to a COL I hydrogel and treated cartilage defects in rats. 36 Dasargyri et al used COL I hydrogel enriched with a high density of fetal chondrocytes to culture cartilage tissue in vitro, but the disadvantage of this novel material is the characteristic of auto-contraction. 37…”

Section: Pure Collagen Hydrogels Loaded With Chondrocytesmentioning

confidence: 99%

Collagen‐Based Hydrogels for Cartilage Regeneration

Sun,

Xu,

Han

et al. 2023

Orthopaedic Surgery

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Cartilage regeneration remains difficult due to a lack of blood vessels. Degradation of the extracellular matrix (ECM) causes cartilage defects, and the ECM provides the natural environment and nutrition for cartilage regeneration. Until now, collagen hydrogels are considered to be excellent material for cartilage regeneration due to the similar structure to ECM and good biocompatibility. However, collagen hydrogels also have several drawbacks, such as low mechanical strength, limited ability to induce stem cell differentiation, and rapid degradation. Thus, there is a demanding need to optimize collagen hydrogels for cartilage regeneration. In this review, we will first briefly introduce the structure of articular cartilage and cartilage defect classification and collagen, then provide an overview of the progress made in research on collagen hydrogels with chondrocytes or stem cells, comprehensively expound the research progress and clinical applications of collagen‐based hydrogels that integrate inorganic or organic materials, and finally present challenges for further clinical translation.

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Evaluation of Collagen Gel-Associated Human Nasal Septum-Derived Chondrocytes As a Clinically Applicable Injectable Therapeutic Agent for Cartilage Repair

Cited by 7 publications

References 40 publications

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure

Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

Collagen‐Based Hydrogels for Cartilage Regeneration

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