Evaluation of Clostridium difficile Infection with PET/CT Imaging in a Mouse Model

Cussó, Lorena; Reigadas, Elena; Muñóz, Patricia; Desco, Manuel; Bouza, Emilio

doi:10.1007/s11307-019-01408-4

Cited by 5 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, we could not observe a direct correlation between the degree of motor activity and the amount of brain uptake. Another limitation was that we did not test hormone levels or stress indicators that might have affected the [18F]FDG brain uptake [ 3 , 26 ]. Moreover, we could not rule out the possibility that the animals might have been affected by other stimuli, such as noise or odours, which were not specifically controlled during the uptake or the recording period.…”

Section: Discussionmentioning

confidence: 99%

“…Positron emission tomography (PET) is a non-invasive imaging technique that enables in vivo studies of many physiological and pathological processes, such as Alzheimer disease [ 1 ], cancer [ 2 ], or infectious diseases [ 3 ]. In both clinical and preclinical settings, the radiotracer most widely used to study brain glucose metabolism with PET is 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG), due to its numerous clinical indications [ 4 ] and its high sensitivity and availability.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of illumination level [18F]FDG-PET brain uptake in free moving mice

et al. 2021

Self Cite

View full text Add to dashboard Cite

In both clinical and preclinical scenarios, 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) is the radiotracer most widely used to study brain glucose metabolism with positron emission tomography (PET). In clinical practice, there is a worldwide standardized protocol for preparing patients for [18F]FDG-PET studies, which specifies the room lighting. However, this standard is typically not observed in the preclinical field, although it is well known that animal handling affects the biodistribution of [18F]FDG. The present study aimed to evaluate the effect of ambient lighting on brain [18F]FDG uptake in mice. Two [18F]FDG-PET studies were performed on each animal, one in light and one in dark conditions. Thermal video recordings were acquired to analyse animal motor activity in both conditions. [18F]FDG-PET images were analysed with the Statistical Parametric Mapping method. The results showed that [18F]FDG uptake is higher in darkness than in light condition in mouse nucleus accumbens, hippocampus, midbrain, hindbrain, and cerebellum. The SPM analysis also showed an interaction between the illumination condition and the sex of the animal. Mouse activity was significantly different (p = 0.01) between light conditions (632 ± 215 s of movement) and dark conditions (989 ± 200 s), without significant effect of sex (p = 0.416). We concluded that room illumination conditions during [18F]FDG uptake in mice affected the brain [18F]FDG biodistribution. Therefore, we highlight the importance to control this factor to ensure more reliable and reproducible mouse brain [18F]FDG-PET results.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of illumination level [18F]FDG-PET brain uptake in free moving mice

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yamato and co-workers have shown that 2-[ 18 F]FDG PET was able to visualise small bowel ulceration caused by non-steroidal anti-inflammatory drugs (NSAID) in rats ( 19 ). In mice with severe colonic clostridium difficile infection, the marked increase in 2-[ 18 F]FDG uptake correlated with the clinical condition ( 20 ). To our knowledge, the characteristics and dynamics of chemotherapy-induced mucositis over time has not previously been studied with PET.…”

Section: Introductionmentioning

confidence: 99%

PET/CT imaging detects intestinal inflammation in a mouse model of doxorubicin-induced mucositis

et al. 2022

View full text Add to dashboard Cite

IntroductionA severe side effect of cancer chemotherapy is the development of gastrointestinal mucositis, characterised by mucosal inflammation. We investigated if 2-deoxy-2-[18F] fluoro-D-glucose positron emission tomography combined with computed tomography (2-[18F]FDG-PET/CT) could visualise gastrointestinal mucositis in mice treated with the chemotherapeutic agent doxorubicin.MethodsIn this study, gastrointestinal inflammation was longitudinally evaluated by 2-[18F]FDG-PET/CT scans before and 1, 3, 6, and 10 days after treatment with doxorubicin. Doxorubicin-treated mice were compared to saline-treated littermates using the abdominal standard uptake value of 2-[18F]FDG corrected for body weight (SUVBW).ResultsAbdominal SUVBW was significantly increased on day 1 (p < 0.0001), day 3 (p < 0.0001), and day 6 (p < 0.05) in the doxorubicin-treated group compared to controls. Abdominal SUVBW returned to baseline levels on day 10. In the doxorubicin group, the largest weight loss was observed on day 3 (control vs doxorubicin, mean percent of baseline weight: (98.5 ± 3.2% vs 87.9 ± 4.6%, p < 0.0001). Moreover, in the doxorubicin-treated group, villus lengths were decreased by 23-28% on days 1 and 3 in the small intestine (p < 0.05), and jejunal levels of tumour necrosis factor and interleukin-1β were significantly increased on day 3 (p < 0.05).DiscussionTogether, these findings indicate that sequential 2-[18F]FDG-PET/CT scans can objectively quantify and evaluate the development and resolution of intestinal inflammation over time in a mouse model of doxorubicin-induced mucositis.

show abstract

Molecular imaging of microbiota-gut-brain axis: searching for the right targeted probe for the right target and disease

Giron

Mazzi

2021

Nuclear Medicine and Biology

View full text Add to dashboard Cite

Evaluation of Clostridium difficile Infection with PET/CT Imaging in a Mouse Model

Cited by 5 publications

References 29 publications

Effect of illumination level [18F]FDG-PET brain uptake in free moving mice

Effect of illumination level [18F]FDG-PET brain uptake in free moving mice

PET/CT imaging detects intestinal inflammation in a mouse model of doxorubicin-induced mucositis

Molecular imaging of microbiota-gut-brain axis: searching for the right targeted probe for the right target and disease

Contact Info

Product

Resources

About