Evaluation of Circulatory Concentration of Cytokines Platelet-Derived Growth Factor-BB, Transforming Growth Factor Beta-1, and IL-10 in Canine Generalized Demodicosis

Abstract

The purpose of this study was to investigate the changes in circulating concentrations of platelet-derived growth factor-BB (PDGF-BB), transforming growth factor beta-1 (TGF-β1) and interleukin-10 (IL-10) in dogs with generalized demodicosis. Twelve dogs with generalized demodicosis were diagnosed based on clinical findings and microscopic examination of the cutaneous scrapings. Six healthy dogs were included in the study as a control. Commercial specific ELISA assays measured circulating concentrations of plasma PDGF-BB, and serum TGF-β1 and IL-10. Marked and significant increase in plasma PDGF-BB and serum TGF-β1 concentrations was evidenced in diseased dogs, while increase in serum IL-10 concentration was non-significant. The levels of all three markers dropped down to near normal/healthy status after the treatment of diseased dogs. These results indicate that the increased concentrations of circulating PDGF-BB, TGF-β1, and IL-10 play a pivotal role in the pathogenesis of the canine demodicosis.

Regional features of the course of demodicosis in dogs

“…In the etiology of skin diseases in dogs, dermatitis of parasitic origin is recorded quite often, which is due to the uncontrolled growth in the number of stray animals, the relatively low effectiveness of treatment, the ecology of large cities, and a number of other factors (Fourie et al, 2019;Parwari et al, 2022). Urban dog populations are intensively affected by negative factors, including a high level of stress, an unsatisfactory ecological situation, feeding with dry and other concentrated feeds, and uncontrolled breeding.…”

“…It has now been established that demodicosis invasion in dogs has a variety of clinical manifestations -from single skin lesions to systemic pathology involving internal organs in the process (Perego et al, 2019;O'Neill et al, 2020;Parwari et al, 2022).…”