Evaluation of Circulating Tumor Cells-DNA Methylated Separation Nano-Polylactic-co-Glycolic Acid Magnetic Beads with Epithelial Cell Adhesion Molecul/Epidermal Growth Factor Receptor Double Antibody Modification and Its Application in Lung Cancer Diagnosis

Hu, Shuqiao; Lin, Jiehuan; Wu, Chaoyang; Ma, Jun; Chen, Shanshan; Chen, Funan

doi:10.1166/sam.2021.4161

Cited by 5 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since there is no need for much permeation or systemic exposure, B4 was a promising candidate. So based on the results of gelling temperature, mucoadhesion, and ex vivo permeation, B4 was selected for in vivo studies [ 37 , 38 , 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of a Nano-Tool Appended Oilmix (Clove and Tea Tree Oil) Thermosensitive Gel for Vaginal Candidiasis

Alkhanjaf

Athar²,

Ullah³

et al. 2022

JFB

View full text Add to dashboard Cite

The main objective of the proposed work was the development of a thermosensitive gel (containing clove and tea tree oil) for the management of vaginal candidiasis. Both oils have been recommended to be used separately in a topical formulation for vaginal candidiasis. Incorporating two natural ingredients (clove and tea tree oil) into a product give it a broad antimicrobial spectrum and analgesic properties. The two oils were mixed together at a 3:1 ratio and converted into o/w nanoemulsion using the aqueous titration method and plotting pseudo ternary phase diagrams. Further transformations resulted in a gel with thermosensitive properties. To determine the final formulation’s potential for further clinical investigation, in vitro analyses (viscosity measurement, MTT assay, mucoadhesion, ex vivo permeation) and in vivo studies (fungal clearance kinetics in an animal model) were conducted. The current effort leveraged the potential of tea tree and clove oils as formulation ingredients and natural therapeutic agents for vaginal infections. Its synergy generated a stable and effective thermosensitive gel that can be utilized for recurrent candidiasis and other infections.

show abstract

Section: Resultsmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of a Nano-Tool Appended Oilmix (Clove and Tea Tree Oil) Thermosensitive Gel for Vaginal Candidiasis

Alkhanjaf

Athar²,

Ullah³

et al. 2022

JFB

View full text Add to dashboard Cite

show abstract

“…EGFR is an ideal candidate for cancer target as it is often overexpressed in cancer cells and the overexpression is associated with advanced disease and poor prognosis. The EGFR-targeted strategy by humanized monoclonal antibodies has been developed against the extracellular domain of EGFR such as Cetuximab (Cet) [ 24 , 25 ]. Cet is a human/murine chimeric monoclonal antibody with an immunoglobulin G1 Fc isotype [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Lin

Raj

et al. 2022

Pharmaceutics

View full text Add to dashboard Cite

Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy.

show abstract

Nanomaterials in Cancer Immunotherapy: A Spotlight on Breast Cancer

Ahmad

Abdel-Wahab

et al. 2023

sci adv mater

View full text Add to dashboard Cite

Breast cancer (BC) is one of the primary causes of death among females worldwide. It can affect a woman at any age after puberty, but the risk of developing the disease increases with age. An early diagnosis and the implementation of an appropriate therapeutic strategy are the two most essential aspects in assuring a favorable prognosis for patients diagnosed with any cancer. There has been significant development in cancer immunotherapy over the past few years. It is among the most effective approaches to fighting cancer and boosts the immune system. In the preclinical setting, immunotherapy using checkpoint blockade antibodies and antigen receptor T cells has shown promising results in BC. Despite this, developing safe and effective immunotherapy against breast cancer is challenging because several novel antigens are poorly immunogenic. Regrettably, conventional immunotherapy confronts further obstacles, such as its inability to trigger the anti-tumor response sufficiently. Most tumors have low immunogenicity due to their origin in healthy cells, making it difficult for the immune system to recognize them as foreign invaders. Additionally, the clinical use of immunotherapy for BC has experienced significant drawbacks, including poor immune responses due to insufficient antigen delivery to the immune cells and uncontrolled immune system regulation, which can promote autoimmunity and nonspecific inflammation. To address these challenges, nanomaterial-based immunotherapy has recently emerged as a potent tool against BC. Scientists have been enthralled by the potential of nanomaterial in BC immunotherapy for decades due to its significant benefits over traditional immunotherapy. Over the past few decades, there has been a considerable increase in the research and application of nanomaterial-based antigens/adjuvants in BC immunotherapy. This review focuses on current advances in BC immunotherapy strategies by focusing on recent breakthroughs in nano immunotherapy.

show abstract

Evaluation of Circulating Tumor Cells-DNA Methylated Separation Nano-Polylactic-co-Glycolic Acid Magnetic Beads with Epithelial Cell Adhesion Molecul/Epidermal Growth Factor Receptor Double Antibody Modification and Its Application in Lung Cancer Diagnosis

Cited by 5 publications

References 25 publications

In Vitro and In Vivo Evaluation of a Nano-Tool Appended Oilmix (Clove and Tea Tree Oil) Thermosensitive Gel for Vaginal Candidiasis

In Vitro and In Vivo Evaluation of a Nano-Tool Appended Oilmix (Clove and Tea Tree Oil) Thermosensitive Gel for Vaginal Candidiasis

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Nanomaterials in Cancer Immunotherapy: A Spotlight on Breast Cancer

Contact Info

Product

Resources

About