“…A method described by Hara and Radin involved the use of n-hexane/2-propanol (3:2, v/v) mixture as the lipid extractant, while Maxwell's method reported the use of dichloromethane and methanol (Hara & Radin, 1978;Maxwell, Mondimore, & Tobias, 1986). Apart from a re-development of Hara and Radin's method, these two procedures have not found wide application in COPs analysis (Ferioli, Caboni, & Dutta, 2008;Ferioli, Dutta, & Caboni, 2010;Ubhayasekera, Tres, Cobony, & Dutta, 2010). In brief, Folch's method is by far the most widely used method, as it continues to be considered the most efficient and reliable technique for the exhaustive isolation of lipids from various tissues (Grau, Cobony, Grimpa, Baucells, & Guardiola 2001;Janoszka, 2010;Mazalli & Bragagnolo, 2007;Mazalli & Bragagnolo, 2009;Obara, Obiedziń ski, & Kołczak, 2006;Sampaio et al 2006).…”