Evaluation of Chlorhexidine Substantivity on Human Dentin: A Chemical Analysis

Effect of Chlorhexidine on Dentin Bond Strength of Two Adhesive Systems after Storage in Different Media

“…Besides presenting a well-known wide antimicrobial spectrum, CHX solutions also have substantivity. Substantivity means that after application the material remains active on the tooth surface for prolonged periods (19), which may add some advantage to the action of CHX in the long term. In contrast, the use of CHX pretreatment has shown controversial results and it is not always able to preserve the adhesive interface stability in the long term (11,17,20).…”

Influence of Chlorhexidine Application Time on the Bond Strength between Fiber Posts and Dentin

“…The adhesive monomers that envelop the collagen fibrils treated with CHX, as well as the presence of an adhesive layer on the hybrid layer, can also contribute to the preservation of CHX at the interface and prolong its inhibitory action (20,21). Substantivity of CHX, or its ability to be retained in dentin matrices (16), could be another reason why CHX-treated dentin may form more stable hybrid layers.…”

“…Chlorhexidine digluconate (CHX) has been proposed as an irrigant in endodontic treatment because of its antimicrobial activity (15), substantivity (16), and also because it does not affect the bond strength of resin composite restorations (17) and root canal sealers to the dentin (18). According to Moreira et al (19), CHX does not promote morphologic structure alterations in the dentin organic matrix of root dentin; thus, it maintains the quality of the dentin substrate for later obturation or restoration of the tooth with resin-based materials.…”

Evaluation of the Effect of Enterococcus faecalis Biofilm on the 2% Chlorhexidine Substantivity: An In Vitro Study

“…The methodology for quantification of CHX was adapted from Souza et al (17). The specimen of each set that was not used for confocal laser scanning microscopy (CLSM) was placed in 1.5-mL microtubes, and 1 mL of an extraction solution (acetonitrile: 1% formic acid in water, 20:80) was added.…”

“…Studies have shown that chlorhexidine (CHX) is able to adsorb onto the dentin walls (substantivity), providing long-lasting antimicrobial effects (12)(13)(14)(15)(16)(17)(18)(19)(20). In this regard, different evaluation periods are considered.…”