Evaluation of chitin and chitosan from insect as feed additives to mitigate ruminal methane emission

Haryati, Ratna Puspita; Jayanegara, Anuraga; Laconi, E. B.; Ridla, Muhammad; Suptijah, Pipih

doi:10.1063/1.5115646

Cited by 17 publications

(25 citation statements)

References 7 publications

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“…This agrees with previous reports which indicated no adverse effect of chitosan on nutrient digestibility when little roughage was used in vivo [22,23] or in vitro [9]. Moreover, Haryati et al [8] noted that CH 4 production decreased when chitosan was added at 2% of substrate. The mode of action by which chitosan reduces CH 4 synthesis in ruminants has been proposed.…”

Section: In Vitro Rumen Parameters and Ch 4 Concentrationsupporting

confidence: 92%

“…A previous experiment by Goiri et al [10] demonstrated that the addition of chitosan can inhibit in vitro ruminal CH 4 synthesis when high concentrations are added to the substrate. Furthermore, adding chitosan at 2% of substrate resulted in a strong reduction effect on CH 4 formation [8].…”

Section: Introductionmentioning

confidence: 93%

“…The mode of action by which chitosan reduces CH 4 synthesis in ruminants has been proposed. This could be due to properties such as chitosan's high degree of deacetylation, which may modify methanogen's cell wall permeability, thus reducing CH 4 production [8]. In addition, positively charged chitosan may interfere with negatively charged methanogens and cause a leakage of protein and other intracellular compositions from the cytosol [9].…”

Section: In Vitro Rumen Parameters and Ch 4 Concentrationmentioning

confidence: 99%

“…Chitosan is a biopolymer found in the exoskeletons of crab and shrimp. Interestingly, chitosan can shift volatile fatty acid (VFA) profiles and change ruminal fermentation, as well as increase C3 concentration and, consequently, reduce CH 4 production [8]. Furthermore, CH 4 reduction could be due to the degree of deacetylation found in the chitosan, which may modify the permeability of methanogenic bacteria cell walls [9].…”

Section: Introductionmentioning

confidence: 99%

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Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Seankamsorn

Cherdthong

Wanapat

2019

Animals

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It was hypothesized that the combination of glycerin and chitosan improves ruminal fermentation efficiency via an enhanced propionate (C3) and reduces in vitro CH4 production. This was explored through in vitro gas production with substrates containing crude glycerin, which replaced cassava chips in the studied ration. The experimental design was organized following a 3 × 3 factorial in completely randomized design and the arrangement of treatments were different levels of crude glycerin supplementations 0, 10.5, and 21% of total mixed ration (TMR) and chitosan levels were added at 0, 1, and 2% dry matter (DM) of substrate. Then, 0.5 g of TMR substrates were added into 40 mL bottles, together with respective doses of chitosan and then incubated at 39 °C. The dietary treatments were performed in three replicates within the incubation, and incubations were repeated on three separate days (runs). No interactions were found between crude glycerin and chitosan doses in terms of theoretical maximum of asymptotic gas production (b), rate of gas production (c), the discrete lag time prior to gas production (L), or the cumulative gas production at 96 h of incubation (p > 0.05). Cumulative gas production at 96 h of incubation was similar among the doses of crude glycerin and levels of chitosan, which ranged from 64.27 to 69.66 mL/g DM basis of substrate (p > 0.05). The concentration of ruminal NH3-N after 2 and 4 h of incubation ranged from 14.61 to 17.10 mg/dL and did not change with the addition of crude glycerin with chitosan (p > 0.05). The concentration of CH4 after 2 h of incubation did not change among treatments (p > 0.05), whereas after 4 h of incubation, CH4 synthesis was significantly reduced by enhancing doses of crude glycerin and chitosan (p < 0.05). The combination of 21% of crude glycerin in TMR with 2% chitosan depressed CH4 production as much as 53.67% when compared to the non-supplemented group. No significant crude glycerin and chitosan interaction effect was detected for in vitro digestibility of nutrients after incubation for 12 and 24 h using the in vitro gas production technique (p > 0.05). In addition, no significant changes (p > 0.05) were observed in total volatile fatty acids, acetate (C2) or butyrate content among treatments and between the main effects of crude glycerin with chitosan. At 4 h of incubation, ruminal C3 content and the C2 to C3 ratio changed significantly when crude glycerin and chitosan was added (p < 0.05). The 21% crude glycerin incorporate into TMR, in combination with 2% additional chitosan, increased C3 content by 26.41%, whereas the ratio of C2 to C3 was reduced by 31% when compared to the control group. Propionate concentration increased by 11.75% when increasing levels of chitosan at 2% of substrate, whereas the C2 to C3 ratio decreased by 13.99% compared to the 0% chitosan group. The inclusion of crude glycerin at 21% in TMR diets with chitosan supplementation at 2% enhanced ruminal propionate concentration and reduced methane production without causing any detrimental effect on the gas kinetics or nutrient digestibility.

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Section: In Vitro Rumen Parameters and Ch 4 Concentrationsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 93%

Section: In Vitro Rumen Parameters and Ch 4 Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Seankamsorn

Cherdthong

Wanapat

2019

Animals

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show abstract

“…Chitosan may be obtained from deacetylation of chitin, which is a biopolymer present in the exoskeleton of crustaceans such as crabs and shrimp. Chitosan is very interesting to study because it can change the profile of volatile fatty acids (VFA) by increasing propionate concentration (C 3 ) and thereby reducing the production of CH 4 (Haryati et al, 2019). Furthermore, the reduction in CH 4 is related to the degree of deacetylation found in chitosan, which can modify the cell wall permeability of methanogenic archaea (Zanferari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Enteric Methane Emissions and Rumen Fermentation Profile Treated by Dietary Chitosan: A Meta-Analysis of In Vitro Experiments

Harahap

Setiawan

Nahrowi

et al. 2020

Trop. Anim. Sci. J.

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Chitosan is a natural compound obtained from deacetylation of chitin, which is a biopolymer present in the exoskeleton of crustaceans such as crabs and shrimp. The present study aimed to perform a meta-analysis from published studies regarding the effects of chitosan on methane emission and rumen fermentation profile of in vitro batch culture experiments. A total of 41 studies from 12 articles were integrated into a database. Parameters included were gas production, methane emission, rumen fermentation characteristics, microbial population, nutrient digestibility, and fatty acid profile. Data were analyzed according to mixed model methodology in which different studies were treated as random effects and chitosan addition levels were treated as fixed effects. Results showed that chitosan addition was able to reduce enteric methane emissions (p<0.001). Such methane decrease was accompanied by a decline in the protozoa population (p<0.05) and a tendency of methanogen reduction (p<0.1). The increasing chitosan level was associated with a decrease in total VFA and ammonia concentrations (both at p<0.001). Chitosan addition decreased acetate proportion (p<0.001) while elevated propionate proportion (p<0.001). Chitosan was associated with an increase of dry matter digestibility, crude protein digestibility, and neutral detergent fiber digestibility (p<0.001). Chitosan increased concentrations of C 18:3n3 (p<0.05), conjugated linoleic acid (p<0.01) and polyunsaturated fatty acids (p<0.01) while decreased concentration of saturated fatty acids (p<0.001). It can be concluded that chitosan addition can mitigate enteric methane emission and alters rumen fermentation profiles in a favorable direction.

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Encapsulation of protein-based bioactive from black soldier fly for ruminant feeding

Muslykhah,

Phupaboon,

Suriyapha

et al. 2024

Journal of Agriculture and Food Research

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Evaluation of chitin and chitosan from insect as feed additives to mitigate ruminal methane emission

Cited by 17 publications

References 7 publications

Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Combining Crude Glycerin with Chitosan Can Manipulate In Vitro Ruminal Efficiency and Inhibit Methane Synthesis

Enteric Methane Emissions and Rumen Fermentation Profile Treated by Dietary Chitosan: A Meta-Analysis of In Vitro Experiments

Encapsulation of protein-based bioactive from black soldier fly for ruminant feeding

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