Evaluation of Chemical Analysis for the Determination of Solasodine in Solarium Laciniatum

Solanum dulcamara L. (Bittersweet): Accumulation of Steroidal Alkaloids in the Plant and in Different in Vitro Systems

“…Estimation of solasodine concentration is often done by colorimetric procedures with bromthymol blue (Coxon et al 1979) or methyl orange (Pandeya et al 1981;Crabbe and Fryer 1982) after direct hydrolysis of plant material.…”

Non-aqueous capillary electrophoresis for the simultaneous analysis of solasodine and solasonine

“…The earliest methods employed gravimetric and titrimetric procedures (Hardman and Williams, 1976;Telek, 1977), but these are insensitive and non-specific. Colorimetric methods were developed later but are non-specific as well (Birner, 1969;Karawya et al, 1975;Lancaster and Mann, 1975;Crabbe and Fryer, 1982). Among chromatographic methods, TLC (Khafagy et al, 1972;Hosoda and Yatazawa, 1979;Nigra et al, 1987;Puri et al, 1994), HPLC (Hunter et al, 1976; and GLC (Hosoda and Yatazawa, 1979) procedures have been developed, but except for the TLC technique, these methods are not used extensively.…”

Transformed Root Cultures of Solanum dulcamara L.: A Model for Studying Production of Secondary Metabolites

“…2.7 Determination of total steroids 2.7.1 Determination of steroidal bases A spectrophotometric assay adopted from a method described by Briner, 1969 and modified by Crabbe & Fryer, 1982, was followed. The method depends on formation of a coloured complex of the steroidal bases with methyl orange, after acid hydrolysis of the glycosides in the extract and its spectrophotometric measurement at 425 nm.…”