1982
DOI: 10.1002/jps.2600711212
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Evaluation of Chemical Analysis for the Determination of Solasodine in Solarium Laciniatum

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Cited by 13 publications
(8 citation statements)
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“…Estimation of solasodine concentration is often done by colorimetric procedures with bromthymol blue (Coxon et al 1979) or methyl orange (Pandeya et al 1981;Crabbe and Fryer 1982) after direct hydrolysis of plant material.…”
Section: Extraction and Identification Of Saponins And Steroidal Alkamentioning
confidence: 99%
“…Estimation of solasodine concentration is often done by colorimetric procedures with bromthymol blue (Coxon et al 1979) or methyl orange (Pandeya et al 1981;Crabbe and Fryer 1982) after direct hydrolysis of plant material.…”
Section: Extraction and Identification Of Saponins And Steroidal Alkamentioning
confidence: 99%
“…The earliest methods employed gravimetric and titrimetric procedures (Hardman and Williams, 1976;Telek, 1977), but these are insensitive and non-specific. Colorimetric methods were developed later but are non-specific as well (Birner, 1969;Karawya et al, 1975;Lancaster and Mann, 1975;Crabbe and Fryer, 1982). Among chromatographic methods, TLC (Khafagy et al, 1972;Hosoda and Yatazawa, 1979;Nigra et al, 1987;Puri et al, 1994), HPLC (Hunter et al, 1976; and GLC (Hosoda and Yatazawa, 1979) procedures have been developed, but except for the TLC technique, these methods are not used extensively.…”
Section: Introductionmentioning
confidence: 99%
“…2.7 Determination of total steroids 2.7.1 Determination of steroidal bases A spectrophotometric assay adopted from a method described by Briner, 1969 and modified by Crabbe & Fryer, 1982, was followed. The method depends on formation of a coloured complex of the steroidal bases with methyl orange, after acid hydrolysis of the glycosides in the extract and its spectrophotometric measurement at 425 nm.…”
Section: Extraction and Isolation Of Steroidal Compoundsmentioning
confidence: 99%