Evaluation of changes in the locomotion and histology of sciatic nerve following experimental autoimmune encephalomyelitis

Abstract

Introduction: Involvement of peripheral nerves in the experimental model of multiple sclerosis (MS) is rarely observed. The objective of this study was to investigate the changes in the locomotion in a mouse model of experimental autoimmune encephalomyelitis (EAE) and correlate with histological changes, if any, in the sections of sciatic nerve and lumbar part of spinal cord. Material and Methods: C57BL/6 mice (10 weeks, n = 8) were immunized with single subcutaneous injection of 300 μg of MOG35-55 and 200 μL of complete Freund's adjuvant (CFA) to produce EAE models. Limp tail with weakness of hindlimb was observed on day 10 and improvement in the weakness was observed on day 20 onwards. Footprint analysis was done to evaluate the impairment in the locomotion on day 0, 5, 10, 15 and 20 of the experiment. Results: One way repeated measure ANOVA found significant reduction in the mean hindlimb stride length on day 10 and 15 (left) and on day 15 and day 20 (right) when compared to mean stride length in day 0 (p<0.05). Histological analysis showed evidence of macrophage infiltration around the dilated blood vessels in the epineurium of sciatic nerve and evidence of damage in the myelinated white matter of lateral funiculus of the lumbar sections of the spinal cord in EAE mice. Conclusion: It is concluded that in mouse model of EAE, the impairment oflocomotion due to damage in the lumbar part of spinal cord can be associated with inflammatory changes in the sciatic nerve.

Interpretation of the gamma-ray excess and AMS-02 antiprotons: Velocity dependent dark matter annihilations

“…Though traces from the new sector are difficult to be disclosed via the search at collider and the DM direct detection, the indirect search of the gamma-ray line from S 0 d 's decay has the potential to shed light on DM annihilations, with the energy of the gamma-ray line ∼ m S 0 d /2 (about 50−75 GeV). We look forward to more precise observations on the GC gamma-ray line, with the results from the Fermi-LAT [67], the Dark Matter Particle Explorer (DAMPE) [78], the Cherenkov Telescope Array (CTA) [79], the High Energy cosmic-Radiation Detection (HERD) [80,81] and the GAMMA-400 [82,83]. …”

The effect of ellagic acid on spinal cord and sciatica function in a mice model of multiple sclerosis

“…Several case reports and experimental models of MS suggest a reduction in myelin thickness and nerve conduction abnormalities in PNS concurrent to demyelination in CNS. [ 4,5 ]…”

Design and on-orbit status of the trigger system for the DAMPE mission

“…The DArk Matter Particle Explorer (DAMPE) is a spacebased mission designed as a high energy particle detector measuring cosmic rays and γ-rays (Chang et al 2014;Chang et al 2017). The wide dynamical range of energy measurement (5 GeV−10 TeV for electrons/positrons and γ-rays), good energy resolution (1.5% @ 800 GeV for electrons/positrons and γ-rays), and powerful particle identification (PID) capability, make DAMPE one of the most sensitive detectors for indirect detection of dark matter (DAMPE Collaboration et al 2017;Yuan & Feng 2018).…”