Evaluation of celsior solution in experimental liver preservation using ex situ isolated rat liver perfusion

Cascales, P; Fernández-Cornejo, Víctor; Campo, Francisco Sánchez-Del; Torre, Manuel de la; Sánchez-Eixerés, M. Rosa; Soriano, Nicolas; Gonzalez, F. J.

doi:10.1016/s0041-1345(99)00466-2

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…Nevertheless, scarce data are available concerning biochemical issues in organ preservation with Celsior and especially hepatic preservation. In line with the results presented in this study, Cascales et al [16] reported a functional equivalence between Celsior and the UW solution as concluded from a similar recovery of bile production and enzyme loss upon isolated rat liver perfusion. However, our study went further and the aim was to investigate the changes in the metabolic status upon hepatic preservation in the clinically used preservation solutions.…”

Section: Discussionsupporting

confidence: 91%

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Tolba

Akbar²,

Müller³

et al. 2000

Eur Surg Res

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Celsior, a low viscosity and low potassium preservation solution, has recently been tested successfully in the cold preservation of heart, lung, kidney and small intestine. The purpose of the present study was to evaluate the potential of Celsior in the cold preservation of the liver. Livers were harvested from male Wistar rats and then flushed with either Celsior (CE), University of Wisconsin solution (UW) or histidine-tryptophan-α-ketoglutarate solution (HTK) and stored for 24 h at 4°C in the respective solution. The reperfusion was performed in vitro using a recirculating model with oxygenated (95% O₂, 5% CO₂) Krebs-Henseleit buffer at 37°C. To simulate the slow rewarming during the surgical implantation in vivo, all livers were stored for 30 min at room temperature prior to reperfusion. After ischemic storage and also after reperfusion some samples were freeze-clamped for analysis of tissue metabolites while others were tested for structural and functional integrity by the isolated perfusion. CE vs. UW vs. HTK: Metabolic preservation of tissue ATP (μmol/g dry weight) during cold storage was best with Celsior (0.46 ± 0.17 vs. 0.26 ± 0.03 vs. 0.35 ± 0.07; p < 0.05 CE vs. UW), but upon reperfusion energetic recovery was comparable in the three groups (3.45 ± 0.66 vs. 4.27 ± 0.41 vs. 3.63 ± 0.64 μmol/g/dry weight). There appeared to be structural integrity during reoxygenation irrespective of the used preservation solution with comparable values of parenchymal enzyme release (ALT: 575 ± 82 vs. 547 ± 106 vs. 593 ± 38 mU/g/l), bile production (18.0 ± 1.0 vs. 18.5 ± 2.5 vs. 18.7 ± 1.4 μl/g/ min), and the release of acid phosphatase, an indicator for activated Kupffer cells (89 ± 13 vs. 90 ± 5 vs. 123 ± 21 mU/g/l) in this in vitro model. Vascular flow characteristics were approximated by the portal perfusion pressure, which tended to be elevated upon initial reperfusion in the UW group (8.4 ± 0.6 mm Hg) compared to 6.6 ± 1.0 and 7.3 ± 0.4 mm Hg in Celsior and HTK, respectively. However, the pressure values decreased to the normal range even in the UW group with ongoing perfusion. The sensitivity of our model in detecting protective effects of the tested solution was confirmed by a negative control group of livers stored in Ringer’s solution at 4°C, yielding an impaired recovery which differed by one magnitude from the three other groups. Within the limits of an in vitro study it is concluded from these results that Celsior may become a suitable alternative for liver preservation and further studies including a transplantation in vivo are strongly encouraged.

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Section: Discussionsupporting

confidence: 91%

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Tolba

Akbar²,

Müller³

et al. 2000

Eur Surg Res

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“…Even so, our results were in accordance with data from a clinical study in human liver transplantation and in an isolated rat-liver model. Neither study found a difference in the release of intracellular enzymes (LDH, alanine aminotransferase, aspartate aminotransferase) after long-term preservation in CS and UW [5,151. Additionally, our results for HTK were in accordance with data from human liver transplantations, which confirmed a significantly higher release of intracellular enzymes than for UW [25].…”

Section: Discussionmentioning

confidence: 72%

Celsior solution compared with University of Wisconsin solution (UW) and histidine?tryptophan?ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia?reperfusion injury

Janssen¹,

Janssen²,

Broelsch³

2003

Transplant International

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Celsior, a new preservation solution in thoracic organ transplantation was evaluated for efficacy in cold preservation of human hepatocytes and compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK, Custodiol). Human hepatocyte cultures were preserved at 4 degrees C in Celsior, UW and HTK for 2, 6, 12, 24 and 48 h with 6 h of reperfusion. Levels of lactate dehydrogenase (LDH; cell necrosis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; mitochondrial function), and adenosine 5'-triphosphate (ATP; loss of intracellular energy) were measured. Cell necrosis, mitochondrial dysfunction, and loss of ATP were significantly ( P<0.001, P<0.001, P<0.002, respectively) lower in Celsior than in HTK. The amount of cell necrosis and mitochondrial dysfunction in Celsior solution (CS) and UW was equal ( P=n.s.) up to 24 h and significantly lower in UW after 48 h ( P<0.001). Additionally, the intracellular level of ATP was significantly higher after ischemia ( P<0.001) and reperfusion from long-term ischemia (24, 48 h) ( P<0.002). We can conclude that Celsior was superior to HTK and equal to UW in the protection of human hepatocytes against cold preservation injury from ischemia and reperfusion. Furthermore, Celsior was effective in long-term preservation of human hepatocytes.

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“…With respect to electrolyte composition, Celsior is unique in combining high sodium with low potassium content. Celsior has shown to be effective in hypothermic organ preservation in both experimental and clinical settings [2,[4][5][6][7][8][9][10][11][12][13][14][15][16]. However, little information is available on the comparison of Celsior, HTK and UW solutions.…”

Section: Introductionmentioning

confidence: 99%

In vitro function of porcine carotid arteries preserved in UW, HTK and Celsior solutions

Abrahamse

Dinant

Pfaffendorf

et al. 2002

Fundamemntal Clinical Pharma

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We compared the efficacy of histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) solution with Celsior solution using hypothermically-preserved porcine carotid arteries and studied the importance of different components of these solutions by preserving carotid arteries in modified HTK solutions. Excised carotid arteries were stored at 4 degrees C in 0.9% (w/v) NaCl, UW, HTK, Celsior, or a modified HTK solution for up to 14 days. Preservation-induced changes in smooth muscle cell and endothelial cell function were determined using an organ bath for isometric tension recording. Short-term preservation (1-3 days) in UW, HTK and Celsior did not significantly alter contractile and relaxation responses of arterial segments when compared to freshly-excised segments, but significantly impaired these responses in arterial segments stored in 0.9% (w/v) NaCl solution. Long-term hypothermic preservation of arterial segments (7 and 14 days) in 0.9% (w/v) NaCl and HTK solution almost completely abolished all responses, but only slightly reduced the responses of arterial segments stored in UW solution. Intermediate results were obtained for Celsior. Modifying HTK by replacement of chloride for sulfate and phosphate resulted in improved contractile and relaxation responses after long-term preservation. With respect to smooth muscle and endothelial function, UW is superior to HTK and Celsior and the absence of chloride or presence of sulfate and phosphate plays a relevant role in this in vitro model of hypothermic preservation of porcine carotid arteries.

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Evaluation of celsior solution in experimental liver preservation using ex situ isolated rat liver perfusion

Cited by 6 publications

References 5 publications

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Celsior solution compared with University of Wisconsin solution (UW) and histidine?tryptophan?ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia?reperfusion injury

In vitro function of porcine carotid arteries preserved in UW, HTK and Celsior solutions

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