Evaluation of cellular immune response provoked by experimental infection with Cryptosporidium spp. in mice

Al-Warid, Harith Saeed; Mahmood, Souhaila H.; Al-Saqur, Ihsan M.

doi:10.24126/jobrc.2014.8.1.289

Cited by 1 publication

(1 citation statement)

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“…The Same result was obtained from Al-Zarkoushi and Al-Zubaidi (2021b) on quails intestinal tissue that was infected with coccidian. Al-Warid et al (2014) conclouded that lymphocyte and phagocytosis are good indicter to evaluate the cellular immune response in experimentally mice with Cryptosporidium spp. This result disagreed with Cui et al (2018) who found numbers of C. avium oocysts cover the epithelial cell of the small intestine without any pathological change in the epithelial cell.…”

Section: Histopathological Examinationmentioning

confidence: 99%

Biology, Histopathology and Treatment Evaluation Against Cryptosporidium meleagirids on Infected Quails

Yahya¹,

Al-Zubaidi²

2023

AAVS

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Cryptosporidium is a ubiquitous protozoan parasite causing gastrointestinal disorders in various hosts worldwide. The current study was undertaken to study the biology of Cryptosporidium meleagridis and examine the anti-Cryptosporidial efficacy of curcumin in experimentally infected quails compared with that of paromomycin. This study carries out from September 2022 to January 2023 in Baghdad city, Iraq. Oocysts were isolated from naturally infected quails identified as Cryptosporidium meleagridis and used as a strain in experimental infection, about fifty birds were divided into five groups (G1: control negative group (con-), G2: control positive group (con+), G3: infected and treated with paromomycin, G4: infected and treated with curcumin and G5: uninfected and received curcumin). The oocyst shedding was recorded daily for 30 days. At 3,8,14, and 23 days post-infection (DPI), two quails from each group were killed humanely; ileum tissue samples were collected before and processed for histopathological evaluation. Improvement of tissue and the number of oocysts on the villi decreased after treatment. In comparison with G2, oocyst shedding was stopped at the end of the treatment period in both G3 and G4 without recurrence 10 days after drug withdrawal. The pre-patient periods (p.p) was 8-9 DPI. The maximum intensity was 11,000 oocysts per gram of feces. The first clinical sign was detected on 8 th DPI. Top of shedding occurred on 14 DPI, The patient period was 16 DPI. No clinical signs appeared on experimentally infected birds, (incubation period of 8 days). Histopathology examination of the infected ileum showed the development stages of C. meleagridis. There were significant differences between the curcumin (4.33 mg/kg/day) and paromomycin (100 mg/kg/day) treatments. Curcumin was able to treat the infection and needed father evaluation.

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Section: Histopathological Examinationmentioning

confidence: 99%