Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21

Gangwar, Rishabh; Rasool, Shafaq; Mishra, Saroj

doi:10.1016/j.btre.2016.10.002

Cited by 14 publications

(5 citation statements)

References 51 publications

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“…These amino-acid compositions vary from one geographic region to another. Laccase enzyme which is known to play a key role in biosynthesis of melanin has been also reported from Termitomyces (Bose et al 2007; Gangwar et al 2016). Rahmad et al (2014) identified sulphite reductase enzyme from T. heimii which plays a key role in sulphur assimilation.…”

Section: Discussionmentioning

confidence: 92%

Purification and characterisation of a sulphur rich melanin from edible mushroom Termitomyces albuminosus Heim

2018

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Production, purification and characterisation of a black pigment from Termitomyces albuminosus as melanin is reported, for the first time, from shaken submerged culture condition using scanning electron microscopy (SEM), elemental analysis, ultraviolet–visible (UV-VIS), and Fourier transformed infrared spectroscopy (FTIR), electron paramagnetic resonance (EPR) and 13C (CP/MAS) NMR spectra. SEM results on T. albuminosus revealed nanogranular nature of melanin nanoparticles within size range of 400–100 nm with fractal dimension D = 1.195–1.73. Elemental analysis of melanin indicated 54.6% C, 3.5% H, 2.4% N, 26.9% O, and 12% S. UV-VIS and FTIR spectra confirmed to the characteristic of melanin and were identical to the reference commercial sepia melanin. Further validation of the identity of pigment as melanin was achieved by EPR analysis. Termitomyces albuminosus melanin is postulated to be DOPA-type melanin confirmed by 13C (CP/MAS) NMR spectral analysis showing chemical shift at 200–170 ppm carbonyl, 160–110 ppm aromatic region, and with high 40–30 ppm open chain aliphatic region. Chemical modification through oxidation and cysteinylation (Pheomelanin) is implied as indicated by relatively high sulphur content (12%).

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Section: Discussionmentioning

confidence: 92%

Purification and characterisation of a sulphur rich melanin from edible mushroom Termitomyces albuminosus Heim

2018

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“…Screened fungal isolate was subjected to quantitative estimation of lignolytic enzymes following standard protocol i.e., cellulase [28] and xylanase [29]. Fungal isolates were grown in Czapek-dox broth (MgSO4.7H2O -0.5g/L;FeSO4 -0.01g/L;KCl -0.5g/L;KH2PO4 -1g/L ;NaNO3-3g/L;pH -6.0-6.5) with 1% of respective carbon source i.e., carboxy methyl cellulose (CMC) for cellulase and xylan for xylanase.…”

Section: Quantitative Assay Of Ligninolytic Enzymesmentioning

confidence: 99%

“…produces ligninolytic enzymes i.e. cellobiose dehydrogenase, xylanase and other lignocellulosic enzymes exudated by the fungus [29,35,36]. Reason behind dye decoloration might be due to the enzymatic degradation and Congo red degraded products might be adsorbed with the cell wall material of fungus.…”

Section: Termitomycesmentioning

confidence: 99%

Decolorisation of Azo Dye Congo Red (CR) by Termitomyces sp. Biomass

Jackson

Gomathi

2021

IJECC

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Aims: A study was conducted to evaluate decoloration of azo dye, Congo Red (CR) using fungal hyphal mat of beneficial bacidiomycete Termitomyces sp. TMS7 (MW694830) as bio sorbent material. Study design: Completely randomized block design (CRD). Place and duration of study: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, between September 2019 and January 2020. Methodology: Isolation of white rot fungus from basidiocarb was done and screened based on their ligninolytic enzyme activity and Isolate TMS 7 was selected as best isolate and identified through ITS 1 and ITS 4 primers. Efficiency of fungal biomass to decolorize Congo red was assessed and per cent decoloration and kinetics were calculated. Results: Twelve fungal isolates were obtained and Isolate TMS 7 was selected as best isolate based on enzymatic activity. TMS 7 was identified as Termitomyces sp. using ITS 1 and ITS 4 primer. Ligninolytic enzymes i.e. cellulase (9.97 µ mol of glucose released/min/mg protein), and xylanase (9.55 µ mol of xylose released/min/mg protein) were quantified from the crude fungal extract of TMS 7, which was higher than standard (Termitomyces albuminosus -MTCC 1366). Decolorisation efficiency of termitomyces fungal biomass (1 g/100 ml) against different concentration of congo red dye (50-250 mg/L) was assessed. About 100 % (99.9) degradation was recorded in the minimum dye concentration of 50 mg/L within 3 days and 8 % decoloration was achieved at the highest dye concentration (250 mg/L) within 5 days. Conclusion: Possible mechanism of degradation is the presence of lignolytic enzyme especially cellulase, xylanase in the culture filtrate and bio sorption of degraded product by the fungal cell wall components viz., chitin, glucan other complex polymers.

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“…Laccase (LAC, EC 1.10.3.2), p-diphenol oxidase, needs to be added simultaneously into the system as a regenerative enzyme to oxidise redox mediators that serve as an electron acceptor for CDH [10,11]. This enzyme is included in multicopper oxidases (MCOs) and can oxidise organic and inorganic compounds such as mono-, di, poly, amino-, and methoxyphenols and several aromatic amines [12] with concurrent reduction of molecular oxygen to water.…”

Section: Introductionmentioning

confidence: 99%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

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Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications.

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Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21

Abstract: HighlightsUse of culture filtrates of Termitomyces sp. OE147.Decolourization and degradation of Reactive blue 21.Cellobiose dehydrogenase and laccase as effective redox couple.

Cited by 14 publications

References 51 publications

Purification and characterisation of a sulphur rich melanin from edible mushroom Termitomyces albuminosus Heim

Purification and characterisation of a sulphur rich melanin from edible mushroom Termitomyces albuminosus Heim

Decolorisation of Azo Dye Congo Red (CR) by Termitomyces sp. Biomass

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

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