Evaluation of Cell Viability Effects and Antioxidant Activity of Caryota urens Linn. and Couroupita guianensis aublet Leaves in a Model of Ehrlich Ascites Carcinoma as a Xenograft model

Abstract

Plants used in traditional medicine have been identified as the primary source of anticancer agents. One of the cancer-related parameters is the cell viability parameter. Current study aimed to evaluate cell viability effects and antioxidant activity of Caryota urens Linn. and Couroupita guianensis aublet leaves using xenograft model by transplantation of ehrlich ascites carcinoma (EAC) cells into mice. Animals were randomly divided into seven groups of six animals each. For 10 days, EAC cells (2 × 106 cells/mouse) were injected i.p. into each mouse in each group except the normal control and vehicle control groups. The treatment drugs were compared with standard 5-fluorouracil. Group I received water as a control, group II received 0.9% normal saline, group III received 0.5% CMC, group IV received EAC cells as a model control group, group V received EAC cells with 5-flourouracil treatment, group VI received EAC cells with C. urens linn extract, and group VII received EAC cells with C. guianensis aublet. After 10 days of treatment, animals were sacrificed, ascitic fluid was collected for evaluation of cell viability effects, blood collected for hematological parameter estimation, and liver tissue was collected for histopathological study and evaluation of antioxidant activity. Study results of C. urens linn and C. guianensis aublet both shows significant positive effects on cell viability and antioxidant activity. In the study visible differences were observed in liver tissue of different groups. Disease control groups showed damaged liver cells, while the treatment group showed less damage than disease control. Both plants produced positive effects on cell viability and antioxidant activity, resulting in a decrease in viable cell count.