Evaluation of cell interaction with polymeric biomaterials based on hyaluronic acid and chitosan

Abstract

Tissue engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for in vitro cell growth, and functional tissue development, that is subsequently implanted into patient. The aim of the current study was to evaluate the initial aspects of cell interaction with the polymeric biomaterials blends based on hyaluronic acid with chitosan. The hypothesis approach involves synthesis and analysis of swelling and thermal degradation (thermal gravimetric analysis) of the polymer blend; and Vero cell interaction with the biomaterial, through analysis of cytotoxicity, adhesion and cell morphology. The blend resulted in a biomaterial with a high swelling ratio that can allow nutrient distribution and absorption. The thermal gravimetric analysis results showed that the blend had two stages of degradation at temperatures very close to those observed for pure polymers, confirming that the physical mixing of hydrogels occurred, resulting in the presence of both hyaluronic acid and chitosan in the blend. The evaluation of indirect cytotoxicity showed that the blend was non cytotoxic for Vero cells, and the quantitative analysis performed with the MTT could verify a cell viability of 98%. The cells cultured on the blend showed adhesion, spreading and proliferation on this biomaterial, distinguished from the pattern of the control cells. These results showed that the blends produced from hyaluronic acid and chitosan hydrogels are promising for applications in tissue engineering, aiming at future cartilaginous tissue.

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

“…After adding each formulation to Vero cells, they were incubated for 24 h at 37 °C in a 5% CO 2 atmosphere. The cells in the culture medium without any treatment were employed as the negative control, and a solution of phenol (0.25%) was used as a positive control (cytotoxic) [47,48,49]. After Vero cell incubation for 24 h with the PL/CS hydrogel and GSNO-PL/CS hydrogel, a volume of 100 µL of 10% MTT solution (5 mg·mL −1 ) in PBS was added to each of the wells, and the cells were incubated for an additional 4 h. After removing MTT solution, 50 μL of dimethyl sulfoxide was added to each well for 10 min, and the absorbance at 570 nm was measured with an automated spectrophotometric microtiter plate reader (SpectraMax M5, Molecular Devices, Sunnyvale, CA, USA).…”

“…The morphology of Vero cells after 24 h incubation with pure PL/CS or GSNO-PL/CS hydrogels (at final hydrogel concentration of 0.2–9.5 µg·mL −1 ) was observed with an inverted microscope (AxioVert A1, Zeiss, Jena, Germany) and phase contrast [49,50].…”

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

“…This phenome is reversible [21,23]. Due to these properties, it is possible to incorporate either hydrophilic [22,40,49,54] or hydrophobic drugs into PL hydrogels [55].…”

“…The morphology of Vero cells after 24 h incubation with pure PL/CS or GSNO-PL/CS hydrogels (at final hydrogel concentration of 0.2 -9.5 µg•mL -1 ) was observed with an inverted microscope (AxioVert A1, Zeiss) and phase contrast [49,50].…”

“…After adding each formulation to Vero cells, they were incubated for 24 h at 37°C in a 5% CO2 atmosphere. The pure culture medium was employed as a negative control, and a solution of phenol (0.25%) was used as a positive control (cytotoxic)[47][48][49]. After Vero cell incubation for 24 h with the PL/CS hydrogel and GSNO-PL/CS hydrogel, a volume of 100 µL of 10% MTT solution (5 mg•mL -1 ) in PBS was added to each of the wells, and the cells were incubated for an additional 4 h.…”