Evaluation of cell disruption methods in the oleaginous yeasts Yarrowia lipolytica QU21 and Meyerozyma guilliermondii BI281A for microbial oil extraction
CARINA A. TIMOTHEO,
MARIANA F. FABRICIO,
MARCO ANTÔNIO Z. AYUB
et al.
Abstract:The interest for oleaginous yeasts has grown significantly in the last three decades, mainly due to their potential use as a renewable source of microbial oil or single cell oils (SCOs). However, the methodologies for cell disruption to obtain the microbial oil are considered critical and determinant for a large-scale production.Therefore, this work aimed to evaluate different methods for cell wall disruption for the lipid extraction of Yarrowia lipolytica QU21 and Meyerozyma guilliermondii BI281A.The two stra… Show more
“…The reality of using SCOs is far from being achieved, due to the high cost of their recovery from biomass. The costs can be lowered by the usage of a waste carbon source in the culture medium and by a reduction in the operational costs of SCO extraction, which may be accomplished by mechanical methods, chemical and enzymatic methods, or a combination of them [46,47]. Based on the current literature, there is no extraction method that is 100% effective in yielding oils derived from microorganisms.…”
Section: Extraction Of Microbial Oilmentioning
confidence: 99%
“…These methods can help to make the lipid structure more accessible to the solvents, thereby reducing the biggest limitation of the process-the diffusion of the solvent into the raw material [54]. Timotheo et al [47] evaluated liquid nitrogen pre-treated biomass and maceration, followed by ultrasonication extraction, as the treatment with the highest percentage of disrupted cells and the highest oil yield of Y. lipolytica QU21 and Meyerozyma guilliermondii BI281A. Some investigations point out that the lipid yield decreased with increasing pressure, and low pressure (200 MPa) collapsed the cells, while high pressure (400 MPa) created protrusions on the cell wall and the cell fragments spread into the environment [55].…”
Plant and animal oils and fats currently dominate the edible oil market, but a new sustainable alternative of lipids from single-celled organisms has become advantageous in human nutrition and pharmacy. Single-cell oils (SCOs) are lipids biosynthesized and accumulated in the lipid bodies of oleaginous species of bacteria, yeasts, molds, and algae. The review has investigated SCOs’ composition, with a detailed review of the described beneficial impact in medicine, cosmetics, pharmacy, and nutrition. Although microbial oil has been known for more than 100 years, it was not applied until the 21st century, when commercial SCO production for human use started and administrative regulations governing their use were completed. This article discusses the applications of SCOs, which can be easily found in microorganisms, in the pharmaceutical, cosmetic, and food industries. In addition, some aspects of 15- or 17-carbon-atom-long fatty acids were also pointed out. Furthermore, some challenges for heterotrophic single-cell oil synthesis and improvements in its extraction efficiency have also been concluded, which can further contribute to their broadened use in pharmacy, medicine, cosmetics, and food applications.
“…The reality of using SCOs is far from being achieved, due to the high cost of their recovery from biomass. The costs can be lowered by the usage of a waste carbon source in the culture medium and by a reduction in the operational costs of SCO extraction, which may be accomplished by mechanical methods, chemical and enzymatic methods, or a combination of them [46,47]. Based on the current literature, there is no extraction method that is 100% effective in yielding oils derived from microorganisms.…”
Section: Extraction Of Microbial Oilmentioning
confidence: 99%
“…These methods can help to make the lipid structure more accessible to the solvents, thereby reducing the biggest limitation of the process-the diffusion of the solvent into the raw material [54]. Timotheo et al [47] evaluated liquid nitrogen pre-treated biomass and maceration, followed by ultrasonication extraction, as the treatment with the highest percentage of disrupted cells and the highest oil yield of Y. lipolytica QU21 and Meyerozyma guilliermondii BI281A. Some investigations point out that the lipid yield decreased with increasing pressure, and low pressure (200 MPa) collapsed the cells, while high pressure (400 MPa) created protrusions on the cell wall and the cell fragments spread into the environment [55].…”
Plant and animal oils and fats currently dominate the edible oil market, but a new sustainable alternative of lipids from single-celled organisms has become advantageous in human nutrition and pharmacy. Single-cell oils (SCOs) are lipids biosynthesized and accumulated in the lipid bodies of oleaginous species of bacteria, yeasts, molds, and algae. The review has investigated SCOs’ composition, with a detailed review of the described beneficial impact in medicine, cosmetics, pharmacy, and nutrition. Although microbial oil has been known for more than 100 years, it was not applied until the 21st century, when commercial SCO production for human use started and administrative regulations governing their use were completed. This article discusses the applications of SCOs, which can be easily found in microorganisms, in the pharmaceutical, cosmetic, and food industries. In addition, some aspects of 15- or 17-carbon-atom-long fatty acids were also pointed out. Furthermore, some challenges for heterotrophic single-cell oil synthesis and improvements in its extraction efficiency have also been concluded, which can further contribute to their broadened use in pharmacy, medicine, cosmetics, and food applications.
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