Evaluation of cationic nanosystems with melatonin using an eye-related bioavailability prediction model

Hafner, Anita; Lovrić, Jasmina; Romić, Marieta Duvnjak; Juretić, Marina; Pepić, Ivan; Cetina-Čižmek, Biserka; Filipović-Grčić, Jelena

doi:10.1016/j.ejps.2015.04.003

Cited by 43 publications

(22 citation statements)

References 43 publications

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“…Positive charge might also irritate cells, and particularly chitosan has been extensively studied in this respect. In earlier work we have reported on the pronounced permeability enhancing effect of chitosan solutions (Hagesaether, 2011), but this effect seems to diminish when liposomes are coated with chitosan, in line with earlier studies on chitosan nanoparticles (Hafner et al, 2015). This effect might also have been reflected in the biocompatibility, as chitosan in a free soluble form is reported to be much more cytotoxic than when it is incorporated in a nanosystem, arguing for an acceptable cytotoxicity profile of chitosan nanoparticles (Hafner et al, 2015;Pradines et al, 2015).…”

Section: Biocompatibilitysupporting

confidence: 83%

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Adamczak

Hagesæther

Smistad

et al. 2016

International Journal of Pharmaceutics

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Drug delivery to the oral cavity poses a significant challenge due to the short residence time of the formulations at the site of action. From this point of view, nanoparticulate drug delivery systems with ability to adhere to the oral mucosa are advantageous as they could increase the effectiveness of the therapy. Positively, negatively and neutrally charged liposomes were coated with four different types of polymers: alginate, low-ester pectin, chitosan and hydrophobically modified ethyl hydroxyethyl cellulose. The mucoadhesion was studied using a novel in vitro method allowing the liposomes to interact with a mucus-producing confluent HT29-MTX cell-line without applying any external force. MTT viability and paracellular permeability tests were conducted on the same cell-line. The alginate-coated liposomes achieved a high specific (genuine) mucin interaction, with a low potential of cell-irritation. The positively charged uncoated liposomes achieved the highest initial mucoadhesion, but also displayed a higher probability of cell-irritation. The chitosan-coated liposomes displayed the highest potential for long lasting mucoadhesion, but with the drawback of a higher general adhesion (tack) and a higher potential for irritating the cells.

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Section: Biocompatibilitysupporting

confidence: 83%

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Adamczak

Hagesæther

Smistad

et al. 2016

International Journal of Pharmaceutics

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“…A great loss of cell viability was observed for chitosan alone (~40% of cell viability after 24 h of contact with formulations) but a much higher cell viability was observed for chitosan nanoparticles (~80% of cell viability after 24 h of contact with formulations). Similar results were also reported elsewhere using other cell lines [135][136][137]. The low cell viability was probably due to a higher interaction of the free chitosan with negatively charged components of the buccal cells, leading to a higher loss of viability when compared with chitosan nanoparticles that are partially stabilized by the tripolyphosphate [135].…”

Section: Natural Polymerssupporting

confidence: 88%

Novel and revisited approaches in nanoparticle systems for buccal drug delivery

Macedo

Castro

Roque

et al. 2020

Journal of Controlled Release

106

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The buccal route is considered patient friendly due to its non-invasive nature and ease of administration. Such delivery route has been used as an alternative for the delivery of drugs that undergo first-pass metabolism or are susceptible to pH and enzymatic degradation, such as occurs in the gastrointestinal tract. However, the drug concentration absorbed in the buccal mucosa is often low to obtain an acceptable therapeutic effect, mainly due to the saliva turnover, tongue and masticatory movements, phonation, enzymatic degradation and lack of epithelium permeation.Therefore, the encapsulation of drugs into nanoparticles is an important strategy to avoid such problems and improve their buccal delivery. Different materials from lipids to natural or synthetic polymers and others have been used to protect and deliver drugs in a sustained, controlled or targeted manner, and enhance their uptake through the buccal mucosa improving their bioavailability and therapeutic outcome. Overall, the main aim of this review is to perform an overview about the nanotechnological approaches developed so far to improve the buccal delivery of drugs. Herein, several types of nanoparticles and delivery strategies are addressed, and a special focus on pipeline products is also given.

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“…NPs for ophthalmic drug delivery have been shown to enhance the ocular bioavailability while providing sustained release action (Hafner et al, 2015). There have been studies over the years examining the mechanism of drug release and reporting on ocular therapeutic efficacy of drugs delivered using nanoparticles (Zhu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles

Fathalla

Khaled

Hussein

et al. 2015

Drug Development and Industrial Pharmacy

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The aim of this work was to formulate chitosan (CS) based nanoparticles (NPs) loaded with ketorolac tromethamine (KT) intended for topical ocular delivery. NPs were prepared using ionic gelation method incorporating tri-polyphosphate (TPP) as cross-linker. Following the preparation, the composition of the system was optimised in terms of their particle size, zeta potential, entrapment efficiency (EE) and morphology, as well as performing structural characterisation studies using FT-IR and DSC. The data suggested that the size of the NPs was affected by CS/TPP ratio where the diameter of the NPs ranged from 108.0 ± 2.4 nm to 257.2 ± 18.6 nm. A correlation between drug EE and the corresponding drug concentration added to the formulation was observed, where the EE of the NPs increased with increasing drug concentration, for up to 10 mg/mL. FT-IR and DSC revealed that KT was dispersed within the NPs where the phosphate groups of TPP were associated with the ammonium groups of CS. The in vitro release profile of KT from CS NPs showed significant differences (P<0.05) compared to KT solution. Furthermore, mucoadhesion studies revealed adhesive properties of the formulated NPs. The KT loaded NPs were found to be stable when stored at different storage conditions for a period of 3 months. The ex vivo corneal permeation studies performed on excised porcine eye balls confirmed the ability of NPs in retaining the drug on the eye surface for a relatively longer time. These results demonstrate the potential of CS based NPs for the ocular delivery of KT.

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Evaluation of cationic nanosystems with melatonin using an eye-related bioavailability prediction model

Cited by 43 publications

References 43 publications

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells

Novel and revisited approaches in nanoparticle systems for buccal drug delivery

Formulation and corneal permeation of ketorolac tromethamine-loaded chitosan nanoparticles

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