Evaluation of canine intestinal submucosa as a vascular substitute

Lawler, Marion R.; Foster, John; Scott, H. William

doi:10.1016/0002-9610(71)90478-8

Cited by 21 publications

(9 citation statements)

References 10 publications

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“…Instead, we computed the angle, ␣, which represents the change in angle of two lines originally aligned to the orthogonal test axes in the reference configuration using 18 : A total of six fresh specimens, two from each of three pigs, was taken from the proximal jejunum to match the location of the samples used for previous animal studies. [6][7][8][9][10][11][12] Because of the presence of dense vasculature near the mesenteric surface, biaxial samples were cut from the center region of the SIS section, opposite the mesenteric surface. The specimen measured 20 × 20 mm and was tested with the sides aligned to the longitudinal and circumferential directions.…”

Section: Biaxial Mechanical Testingmentioning

confidence: 99%

“…SIS has been used to regenerate canine bladder, 2 for Achilles' tendon repair, 3 for cranial dura mater repair, 4 for abdominal wall repair, 5 and as a vascular graft material. [6][7][8][9][10][11][12] These experimental trials demonstrated that SIS gradually is absorbed by the host organism while concurrently being replaced by host tissue in a configuration different from that of the original implanted material. The alteration in structure is the result of the implant's adaptation to the new forces acting on it as well as its new cellular environment.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of the fiber architecture and biaxial mechanical behavior of porcine intestinal submucosa

Sacks¹,

Gloeckner²

1999

J. Biomed. Mater. Res.

143

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Porcine small intestinal submucosa (SIS) has been shown to serve as a remodelable tissue scaffold in a wide range of applications. Despite the large number of experimental studies, there is a lack of fundamental information on SIS anisotropic mechanical behavior and how this behavior changes postimplantation. As a first step in our study of remodeling biomaterials, we performed biaxial mechanical testing to quantify the anisotropic mechanical behavior and used small-angle light scattering (SALS) to quantify the gross fiber structure of fresh, unimplanted SIS. Structural results indicate that SIS displays primarily a single, continuous preferred fiber direction oriented parallel to the long axis of the intestine. Occasionally, two distinct fiber populations oriented at approximately +/-28 degrees with respect to the longitudinal axis could be distinguished. Consistent with this structure, SIS exhibited a nonlinear, anisotropic mechanical response with higher stresses along the longitudinal axis. Further, the circumferential stress-strain response was strongly affected by the maximum longitudinal strain level, but the maximum circumferential strain level only weakly affected the longitudinal stress-strain response. This asymmetric mechanical coupling suggests strong mechanical interactions on a fiber level. SIS stress-strain response also was similar to glutaraldehyde-treated bovine pericardium, attesting to the substantial strength of SIS in the fresh, untreated state. The results of this study will provide a basis for a future analysis of the structural and mechanical changes during the remodeling process.

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Section: Biaxial Mechanical Testingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of the fiber architecture and biaxial mechanical behavior of porcine intestinal submucosa

Sacks¹,

Gloeckner²

1999

J. Biomed. Mater. Res.

143

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“…This submucosal layer, primarily composed of type I collagen, was proposed as a vascular substitute over 30 years ago [22][23][24] and showed good patency as a large-diameter (10 mm) graft in the canine infrarenal aorta 25 . As a small-diameter (4-5 mm) canine carotid or femoral graft, 75% patency was achieved when systemic anticoagulants were administered in the first eight weeks postsurgery 26 .…”

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confidence: 99%

Remodeling of an acellular collagen graft into a physiologically responsive neovessel

et al. 1999

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Surgical treatment of vascular disease has become common, creating the need for a readily available, small-diameter vascular graft. However, the use of synthetic materials is limited to grafts larger than 5-6 mm because of the frequency of occlusion observed with smaller-diameter prosthetics. An alternative to synthetic materials would be a biomaterial that could be used in the design of a tissue-engineered graft. We demonstrate that a small-diameter (4 mm) graft constructed from a collagen biomaterial derived from the submucosa of the small intestine and type I bovine collagen has the potential to integrate into the host tissue and provide a scaffold for remodeling into a functional blood vessel. The results obtained using a rabbit arterial bypass model have shown excellent hemostasis and patency. Furthermore, within three months after implantation, the collagen grafts were remodeled into cellularized vessels that exhibited physiological activity in response to vasoactive agents.

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“…[15, 16] Research has shown that decellularized SIS contains bioactive factors such as fibronectin, glycosaminoglycan (GAG), and growth factors, [17] as well as bioactive factors involved in cell adhesion, mitogenesis, and chemotaxis. [18] Ligament reconstruction using SIS was demonstrated to be effective in equine, caprine, and canine models.…”

Section: Introductionmentioning

confidence: 99%

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

et al. 2017

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We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix. The xenogeneic decellularized SIS did not display cytotoxicity in vitro and its application prevented NPC degradation. Furthermore, the xenogeneic SIS microparticles were effective in preventing IVD progression in vivo in a rabbit disc degeneration model. In conclusion, our study describes an optimized method for decellularized SIS preparation and demonstrated that the material is safe and effective for treating IVD degeneration.

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Evaluation of canine intestinal submucosa as a vascular substitute

Cited by 21 publications

References 10 publications

Quantification of the fiber architecture and biaxial mechanical behavior of porcine intestinal submucosa

Quantification of the fiber architecture and biaxial mechanical behavior of porcine intestinal submucosa

Remodeling of an acellular collagen graft into a physiologically responsive neovessel

An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration

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