Evaluation of Candida blankii hybrids for biomass production

Meyer, P. S.; Preez, J. C. du; Wingfield, Brenda D.; Kilian, S. G.

doi:10.1016/0168-1656(93)90058-u

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Annually, 60 billion tons of hemicelluloses are produced and remain almost completely unused [15]. It has been reported that the hemicellulose hydrolysate including xylose can be used by Candida blankii for efficient protein production [16]. There are also reports that poly(3-hydroxybutyrate) (PHB) could be synthesized from xylose in Pseudomonas pseudoflava and P. cepacia up to 22% (w w -1 ) and 50% (w w -1 ), respectively [17-19].…”

Section: Introductionmentioning

confidence: 99%

Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

et al. 2012

View full text Add to dashboard Cite

BackgroundPseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain.ResultsThe genes encoding xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli W3110 were introduced into P. putida KT2440. The recombinant KT2440 exhibited a XylA activity of 1.47 U and a XylB activity of 0.97 U when grown on a defined medium supplemented with xylose. The cells reached a maximum specific growth rate of 0.24 h-1 and a final cell dry weight (CDW) of 2.5 g L-1 with a maximal yield of 0.5 g CDW g-1 xylose. Since no mcl-PHA was accumulated from xylose, mcl-PHA production can be controlled by the addition of fatty acids leading to tailor-made PHA compositions. Sequential feeding strategy was applied using xylose as the growth substrate and octanoic acid as the precursor for mcl-PHA production. In this way, up to 20% w w-1 of mcl-PHA was obtained. A yield of 0.37 g mcl-PHA per g octanoic acid was achieved under the employed conditions.ConclusionsSequential feeding of relatively cheap carbohydrates and expensive fatty acids is a practical way to achieve more cost-effective mcl-PHA production. This study is the first reported attempt to produce mcl-PHA by using xylose as the growth substrate. Further process optimizations to achieve higher cell density and higher productivity of mcl-PHA should be investigated. These scientific exercises will undoubtedly contribute to the economic feasibility of mcl-PHA production from renewable feedstock.

show abstract

Section: Introductionmentioning

confidence: 99%

Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Both yeast species are known in the literature for their application in commercial fermentation processes for production of single cell protein and biosurfactants. Using lactose from milk whey as a sole carbon source a considerable production of sophorose lipids (SLs) by means of the yeast Candida bombicola was achieved (8) and the diploid Candida blankii isolates were investigated for their potential to be used in single cell protein production (21,30).…”

Section: Resultsmentioning

confidence: 99%

Thermotolerant Yeast Strains Producers of Galacto-Oligosaccharides

Petrova¹,

Kujumdzieva²

2010

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Application of protoplast fusion to the nonconventional yeast

Kavanagh

Whittaker

1996

Enzyme and Microbial Technology

View full text Add to dashboard Cite

Protuplas~ .fusion hu.s made a significant contribution to our understanding qf the genetics und biochemists? of the rfoncorl~,entional yeasts. and it has ,facilitated the creution ef no\'el strains of yeast thut display enhanced biotechnological potential. This article presents an examination of the means of isolating, reverting. and jikng yeast protoplasts, as well as an analysis of the products resulting jkom such fusions. Although this review is primarily concerned \vith the impact protoplast,fusion has made on our knowledge of the nonconventional yeasts.

show abstract

Evaluation of Candida blankii hybrids for biomass production

Cited by 4 publications

References 27 publications

Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Thermotolerant Yeast Strains Producers of Galacto-Oligosaccharides

Application of protoplast fusion to the nonconventional yeast

Contact Info

Product

Resources

About