Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies

Paivana, Georgia; Barmpakos, Dimitris; Mavrikou, Sophie; Kallergis, Alexandros; Tsakiridis, Odysseus; Kaltsas, G.; Kintzios, Spyridon

doi:10.3390/bios11090345

Cited by 4 publications

(3 citation statements)

References 61 publications

(73 reference statements)

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“…Our research group has previously employed this approach to characterize interactions between breast cancer cells and different chemotherapeutical agents [24,25] as well as neuronal cells and neurotransmitters [26]. In the present study, we compared the Salmonella infection progression in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with a different bacterial multiplicity of infection (MOI) by assessing the alterations of frequencies recordings with impedance spectroscopy measurements.…”

Section: Introductionmentioning

confidence: 99%

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

Marka,

Zografaki,

Papaioannou

et al. 2023

Chemosensors

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A significant number of research papers regarding biosensor-related assays for key food safety pathogens based on the use of mammalian cells has been reported. In this study, the Salmonella typhimurium infection progression was monitored in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with five different bacterial MOI for 30 min by comparing the alterations of frequencies recordings with impedance spectroscopy measurements. For this purpose, bacterial adhesion and invasion assays were initially performed. Then, the data obtained from impedance spectroscopy recordings were compared to cell viability data derived from the MTT uptake cell proliferation assay as well as from live cell analysis assays of mitochondrial membrane potential alterations. From our findings a concentration-dependent increase in bacterial colonies occurring from invaded cells was observed upon a higher multiplicity of infection (MOI) bacterial infection at both cell lines. On the contrary, the bacteria infection did not have any impact on the viability of the cells after 1 h of treatment. Differential results were obtained from the measurement of mitochondrial potential at both cell lines. Finally, the impedance values recorded from the 2D, and 3D cultures were concentration-dependent for both cell lines whereas a characteristic pattern specific to each cell line was revealed. Our results indicate that human cell-based bio-electric assays can be a valuable tool for obtaining a unique fingerprint for each bacterial infection in the near future.

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Section: Introductionmentioning

confidence: 99%

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

Marka,

Zografaki,

Papaioannou

et al. 2023

Chemosensors

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“…In dielectric spectroscopy at low frequencies, the EDL of the electrodes (EEDL) is common to separate from the relaxation of the EDL of the sample. Compensating the EP effect by an algorithm is used to determine the sample’s dielectric constant [ 9 ] and the zeta potential [ 10 ]—another approach to reduce the EP is via the four-electrode technique implying high specific instrumentation [ 11 ]. Furthermore, relaxation times have been used to characterize particles in aqueous suspensions, establishing a relation between the particle size and concentration with the characteristic time [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effective Capacitance from Equivalent Electrical Circuit as a Tool for Monitoring Non-Adherent Cell Suspensions at Low Frequencies

et al. 2022

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Analyzing the electrical double layer (EDL) in electrical impedance spectroscopy (EIS) measurement at low frequencies remains a challenging task for sensing purposes. In this work, we propose two approaches to deal with the EDL in measuring impedance for particles and non-adherent cells in an electrolytic suspension. The first approach is a simple procedure to compute a normalized electrical impedance spectrum named dispersed medium index (DMi). The second is the EIS modeling through an equivalent electric circuit based on the so-called effective capacitance (Cef), which unifies the EDL phenomena. Firstly, as an experiment under controlled conditions, we examine polymer particles of 6, 15, and 48 μm in diameter suspended in a 0.9% sodium chloride solution. Subsequently, we used K-562 cells and leukocytes suspended in a culture medium (RPMI-1640 supplemented) for a biological assay. As the main result, the DMi is a function of the particle concentration. In addition, it shows a tendency with the particle size; regardless, it is limited to a volume fraction of 0.03 × 10−4 to 58 × 10−4. The DMi is not significantly different between K-562 cells and leukocytes for most concentrations. On the other hand, the Cef exhibits high applicability to retrieve a function that describes the concentration for each particle size, the K-562 cells, and leukocytes. The Cef also shows a tendency with the particle size without limitation within the range tested, and it allows distinction between the K-562 and leukocytes in the 25 cells/µL to 400 cells/µL range. We achieved a simple method for determining an Cef by unifying the parameters of an equivalent electrical circuit from data obtained with a conventional potentiostat. This simple approach is affordable for characterizing the population of non-adherent cells suspended in a cell culture medium.

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“…The impedance spectra of living tissues vary due to the differences in morphological structures and components. Therefore, the resistivity spectrum measured at each frequency is highly related to the physiological and pathological characteristics of the tissue [ 5 , 6 , 7 ]. Bioimpedance spectroscopy, a method of measuring electrical properties over a wide range of frequencies, can characterize target tissue.…”

Section: Introductionmentioning

confidence: 99%

Tissue Characterization Using an Electrical Bioimpedance Spectroscopy-Based Multi-Electrode Probe to Screen for Cervical Intraepithelial Neoplasia

Kang

Jung

et al. 2021

Diagnostics

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The successful management of cervical intraepithelial neoplasia (CIN) with proper screening and treatment methods could prevent cervical cancer progression. We propose a bioimpedance spectroscopic measurement device and a multi-electrode probe as an independent screening tool for CIN. To evaluate the performance of this screening method, we enrolled 123 patients, including 69 patients with suspected CIN and 54 control patients without cervical dysplasia who underwent a hysterectomy for benign disease (non-CIN). Following conization, the electrical properties of the excised cervical tissue were characterized using an electrical bioimpedance spectroscopy-based multi-electrode probe. Twenty-eight multifrequency voltages were collected through the two concentric array electrodes via a sensitivity-optimized measurement protocol based on an electrical energy concentration method. The electrical properties of the CIN and non-CIN groups were compared with the results of the pathology reports. Reconstructed resistivity tended to decrease in the CIN and non-CIN groups as frequency increased. Reconstructed resistivity from 625 Hz to 50 kHz differed significantly between the CIN and non-CIN groups (p < 0.001). Using 100 kHz as the reference, the difference between the CIN and non-CIN groups was significant. Based on the difference in reconstructed resistivity between 100 kHz and the other frequencies, this method had a sensitivity of 94.3%, a specificity of 84%, and an accuracy of 90% in CIN screening. The feasibility of noninvasive CIN screening was confirmed through the difference in the frequency spectra evaluated in the excised tissue using the electrical bioimpedance spectroscopy-based multi-electrode screening probe.

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Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies

Cited by 4 publications

References 61 publications

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

Effective Capacitance from Equivalent Electrical Circuit as a Tool for Monitoring Non-Adherent Cell Suspensions at Low Frequencies

Tissue Characterization Using an Electrical Bioimpedance Spectroscopy-Based Multi-Electrode Probe to Screen for Cervical Intraepithelial Neoplasia

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