Evaluation of Brevibacillus brevis as a potential plant growth promoting rhizobacteria for cotton (Gossypium hirsutum) crop

Abstract

The present investigation was undertaken to isolate, screen and evaluate a selected promising PGPR Brevibacillus brevis on cotton crop. Out of 156 bacterial isolates one of the most promising isolate was analyzed for the various PGP traits. A seed germination analysis was conducted with cotton seeds to evaluate the potential of the isolate to promote plant growth. The bacterial isolate was checked for its growth and survival at high temperatures. The isolate was also analyzed for the PGP traits exhibited after the heat treatment. To identify the isolate morphological, biochemical and molecular characterization was performed. The isolate was found positive for many of the PGP attributes like IAA, ARA, anti-fungal activity and ammonia production. Effect of seed bacterization on various plant growth parameters was used as an indicator. The isolate showed significant growth and exhibited various PGP traits at high temperature making it suitable as an inoculant for cotton crop. Isolate was identified as Brevibacillus brevis [SVC(II)14] based on phenotypic as well as genotypic attributes and after conducting this research we propose that the B. brevis which is reported for the first time for its PGP potential in cotton, exerts its beneficial effects on cotton crop through combined modes of actions.

Phyllospheric Microbiomes: Diversity, Ecological Significance, and Biotechnological Applications

“…Zeiller et al (2015) reported that C. botulinum 2301 significantly produce PGPs in a field experiment of clover. A cold-tolerant bacterial strain Exiguobacterium acetylicum 1P promotes wheat seedlings growth (Selvakumar et al 2010), Brevibacillus brevis improve the growth of cotton crop (Nehra et al 2016) and Bacillus spp. induce phosphate solubilization more efficiently when present as endophytes in citrus (Giassi et al 2016).…”

Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates

“…Several microorganisms i.e. Alcaligenes, Arthobacter, Azospirillum, Azotobacter, Bacillus, Burkholderia, Enterobacter, Klebsiella, Pseudomonas, and Serratia support L-Trp metabolism naturally under the impact of plant root exudates including carbohydrates, organic acids, amino acids, mucilages, phenolic compounds, fatty acids, sterols, and vitamins (Nehra et al, 2016;Passari et al, 2016).…”

Characteristics of an endophytic microbial consortium and its impact on rhizosphere microbiota of barley

“…Several Gram-positive bacteria like Bacillus and Brevibacillus species, including B. brevis, produce acyl-homoserine lactonase and are recognized as quorum quenching (Khoiri et al 2017). B. brevis improves plant growth through combined modes of actions, including P solubilization and IAA production (Nehra et al 2016). It cannot be excluded that some members of our multistrain consortium had opposite and conflicting effects.…”