Evaluation of Brazilian Sugarcane Bagasse Characterization: An Interlaboratory Comparison Study

Sluiter, Justin; Chum, Helena L.; Gomes, A.C.A.; Tavares, Renata P. A.; Azevedo, Vinícius; Pimenta, Marcos de Mattos; Rabelo, Sarita Cândida; Marabezi, Karen; Curvelo, A. A. S.; Alves, Aparecido Roberto; Garcia, Wokimar T.; Carvalho, Walter; Esteves, Paula; Mendonça, Simone; Oliveira, Pedro; Ribeiro, José Antônio de Aquino; Mendes, T. D.; Vicentin, Marcos p; Duarte, C.L.; Mori, M.N.

doi:10.5740/jaoacint.15-0063

Cited by 57 publications

(26 citation statements)

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“…Ash, extractives, structural carbohydrates (glucan, xylan, and arabinan) that represent cellulose and hemicelluloses and soluble and insoluble lignin were quantified according to Sluiter et al …”

Section: Methodsmentioning

confidence: 99%

Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production

Menandro

Cantarella

Franco

et al. 2017

Biofuels Bioprod Bioref

142

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Sugarcane straw, consisting of green tops and dry leaves, can be maintained on fields to improve soil quality, or harvested for bioenergy production. The optimum option between these two uses is still uncertain and requires further study. This study, conducted across three crop cycles, provides an assessment of the moisture, nutrients, ash, extractives, cellulose, hemicelluloses, and lignin contents of four sugarcane varieties across seven regions of south‐central Brazil. Suitability of the straw fractions for nutrient recycling, bioelectricity, and second‐generation ethanol production were also evaluated. Results showed that the sugarcane straw yield (dry mass) was 14.0 Mg ha−1, and the ratio of dry straw/fresh stalk was 12%. The composition of green tops and dry leaves differed consistently across varieties, sites, and crop cycles. Dry leaves represented 60% of the straw, but green tops contained about 70% of the total N, P, and K content. Therefore, green tops recycled up to four times more nutrients than dry leaves. Green tops also had six times higher moisture and greater chlorine content which decreased the mill process efficiency. In turn, dry leaves had higher lignin, cellulose, and hemicelluloses content, greater heating value (higher: 17.3 MJ kg−1; lower: 15.6 MJ kg−1) and tended to be a better second‐generation ethanol production feedstock. Overall, the results show that it is preferable to use dry leaves for bioenergy production while leaving green tops on the field for nutrient recycling. This study pointed out that more efficient methods for separating these fractions in the field need to be developed. © 2017 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Methodsmentioning

confidence: 99%

Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production

Menandro

Cantarella

Franco

et al. 2017

Biofuels Bioprod Bioref

142

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“…Cellulose is the main chemical component in natural fibers, followed by hemicellulose and lignin. Table 2 shows the percentages of the chemical composition of the main fibers used as reinforcement in composites [44,45]. Table 2.…”

Section: Chemical Composition Of Natural Fibersmentioning

confidence: 99%

Polymer Composites Reinforced with Natural Fibers and Nanocellulose in the Automotive Industry: A Short Review

Ferreira

Pinheiro

Souza³

et al. 2019

J. Compos. Sci.

131

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Environmental concerns and cost reduction have encouraged the use of natural fillers as reinforcement in polymer composites. Currently, a wide variety of reinforcement, such as natural fibers and nanocellulose, are used for this purpose. Composite materials with natural fillers have not only met the environmental appeal, but also contribute to developing low-density materials with improved properties. The production of natural fillers is unlimited around the world, and many species are still to be discovered. Their processing is considered beneficial since the natural fillers do not cause corrosion or great wear of the equipment. For these reasons, polymer reinforced with natural fillers has been considered a good alternative for obtaining ecofriendly materials for several applications, including the automotive industry. This review explores the use of natural fillers (natural fibers, cellulose nanocrystals, and nanofibrillated cellulose) as reinforcement in polymer composites for the automotive industry.

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“…SCB and PASB were characterized according to the methods of Sluiter et al [85]. The lignocellulosic materials were air-dried to less than 10% (w/w) of moisture content and then milled to obtain particle sizes of 0.12 mm in a knife mill (Pulverisette 19; Fritsch GmbH, Idar-Oberstein, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The analyses were performed in triplicate. The DS for sugar release was calculated as follows: (g/L) of reducing sugar of (Celluclast + Cg AKR-1)/(g/L) of reducing sugars of Celluclast, as described by Goldbeck [85]. …”

Section: Methodsmentioning

confidence: 99%

The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications

Tramontina

Cairo

Liberato

et al. 2017

Biotechnol Biofuels

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BackgroundIn nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps.ResultsIn the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo–keto reductase (CgAKR-1) were comprehensively investigated. CgAKR-1 displayed major structural differences compared with others AKRs, including the differences in the amino acid composition of the substrate-binding site, providing basis for classification as a founding member of a new AKR subfamily (family AKR1 I). Immunolocalization assays with anti-CgAKR-1 antibodies resulted in strong fluorescence in the salivary gland, proventriculus, and foregut. CgAKR-1 supplementation caused a 32% reduction in phenolic aldehydes, such as furfural, which act as fermentation inhibitors of hemicellulosic hydrolysates, and improved ethanol fermentation by the xylose-fermenting yeast Scheffersomyces stipitis by 45%. We observed synergistic enzymatic interactions between CgAKR-1 and commercial cellulosic cocktail for sugarcane bagasse saccharification, with a maximum synergism degree of 2.17 for sugar release. Our data indicated that additive enzymatic activity could be mediated by reactive oxygen species because CgAKR-1 could produce hydrogen peroxide.ConclusionIn summary, we identified the founding member of an AKRI subfamily with a potential role in the termite digestome. CgAKR-1 was found to be a multipurpose enzyme with potential biotechnological applications. The present work provided a basis for the development and application of integrative and multipurpose enzymes in the bioethanol production chain.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0688-6) contains supplementary material, which is available to authorized users.

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Evaluation of Brazilian Sugarcane Bagasse Characterization: An Interlaboratory Comparison Study

Cited by 57 publications

References 0 publications

Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production

Comprehensive assessment of sugarcane straw: implications for biomass and bioenergy production

Polymer Composites Reinforced with Natural Fibers and Nanocellulose in the Automotive Industry: A Short Review

The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications

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