Evaluation of Brassica genotypes for resistance to shatter. II. Variation in siliqua strengh within and between accessions

Kadkol, G. P.; Halloran, G. M.; Macmillan, R. H.

doi:10.1007/bf00035431

Cited by 24 publications

(32 citation statements)

References 4 publications

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“…It is more likely that the enhanced expression of BnaA.FUL was responsible for pod shatter resistance in resistant varieties rather than the depressed expression of BnaC.FUL in R1-1. In general, variations in pod shatter resistance in B. napus were limited; however, there was wide variation in B. rapa (Kadkol et al, 1985). Thus, the expression levels of FUL from the A genome that were correlated with pod shatter resistance may explain this observation.…”

Section: Discussionmentioning

confidence: 84%

Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus

Peng¹,

Li²,

Mei³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. To improve pod shatter resistance in the important oilseed crop Brassica napus, the phenotypic diversity of B. napus was tested using 80 B. napus varieties for pod shatter resistance by a random impact test. Among these varieties, R1-1 was identified as resistant, while R2, 8908B was susceptible to shatter. To understand the molecular basis for this phenotypic difference based on the candidate gene approach, B. napus FRUITFULL (FUL) homologs were identified and characterized. Two FUL loci in the A and C genomes of B. napus were identified. In the susceptible variety, both BnaA.FUL and BnaC.FUL were expressed in the same tissues. However, the expression level of BnaC.FUL differed in varieties with different pod shatter resistance. In the most resistant variety, R1-1, only BnaA.FUL was expressed, while BnaC.FUL was silenced. Therefore, the functional divergence and differing expression of BnaX.FUL homeologs may significantly affect phenotypic variation, which is an important consequence of allopolyploid evolution. This expression level divergence may be useful for selecting pod shatter resistant lines through marker-assisted selection in B. napus-breeding programs.

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Section: Discussionmentioning

confidence: 84%

Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus

Peng¹,

Li²,

Mei³

et al. 2015

Genet. Mol. Res.

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“…Significant differences in measures of shatter tolerance were observed among cultivars of B. napus, B. campestris and B. juncea. However, there was also substantial within plant variation associated with pod size rather than with the position in the inflorescence from which the pod was obtained (Kadkol et al 1985;1989). This indicated that the sampling procedure was crucial to accurate assessments of genotype differences in shatter tolerance, and measurements of few pods from many plants adjusted for pod size was recommended.…”

Section: Adaptation To New Management Strategiesmentioning

confidence: 96%

Physiological Constraints and Their Genetic Manipulation

Thurling¹

1993

Monographs on Theoretical and Applied Genetics

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“…These were then phenotyped using the pendulum pod shatter protocol [15] at the NSW Department of Primary Industries, Wagga Wagga Agricultural Institute (WWAI), Wagga Wagga, Australia. The rupture energy (RE) measures were adjusted for variation in pod length to calculate RELSQ (rupture energy per length squared), as per [25]. The five canola cultivars were also grown in field row plots at the experimental farm of WWAI in two replicates [16], and evaluated for shattering resistance using the same technique, where 10 fully mature pods were sampled per plot.…”

Section: Canola Pod Shattering Resistance Phenotypingmentioning

confidence: 99%

Speed breeding: a powerful tool to accelerate crop research and breeding

Watson

Ghosh

Williams

et al. 2017

Preprint

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The growing human population and a changing environment have raised significant concern for global food security, with the current improvement rate of several important crops inadequate to meet future demand [1]. This slow improvement rate is attributed partly to the long generation times of crop plants. Here we present a method called 'speed breeding', . CC-BY 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/161182 doi: bioRxiv preprint first posted online Jul. 9, 2017; Watson and Ghosh et al. (2017) 2 which greatly shortens generation time and accelerates breeding and research programs.Speed breeding can be used to achieve up to 6 generations per year for spring wheat (Triticum aestivum), durum wheat (T. durum), barley (Hordeum vulgare), chickpea (Cicer arietinum), and pea (Pisum sativum) and 4 generations for canola (Brassica napus), instead of 2-3 under normal glasshouse conditions. We demonstrate that speed breeding in fully-enclosed controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies, and transformation.The use of supplemental lighting in a glasshouse environment allows rapid generation cycling through single seed descent and potential for adaptation to larger-scale crop improvement programs. Cost-saving through LED supplemental lighting is also outlined. We envisage great potential for integrating speed breeding with other modern crop breeding technologies, including high-throughput genotyping, genome editing, and genomic selection, accelerating the rate of crop improvement.For most crop plants, the breeding of new, advanced cultivars, takes several years. Following crossing of selected parent lines, 4-6 generations of inbreeding are typically required to develop genetically stable lines for evaluation of agronomic traits and yield. This is particularly timeconsuming for field-grown crops that are often limited to only 1-2 generations per year. Here, we present flexible protocols for "speed breeding" that use prolonged photoperiods to accelerate the developmental rate of plants [2], thereby reducing generation time. We highlight the opportunity presented by speed breeding and detail protocols to inspire widespread adoption as a state-of-theart breeding and research tool.To evaluate speed breeding as a method to accelerate applied and basic research on cereal species, standard genotypes of spring bread wheat (T. aestivum), durum wheat (T. durum), barley (H. vulgare) and the model grass Brachypodium distachyon were grown in a controlled environment room with extended photoperiod (22 hours light/2 hours dark) ( Fig. 1; Methods:Speed breeding I; Supplementary Table 1). A light/dark period was chosen over a continuous photoperiod to support functional expression of circadian clock genes [3]. Growth was compare...

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Evaluation of Brassica genotypes for resistance to shatter. II. Variation in siliqua strengh within and between accessions

Cited by 24 publications

References 4 publications

Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus

Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus

Physiological Constraints and Their Genetic Manipulation

Speed breeding: a powerful tool to accelerate crop research and breeding

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