Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants

Melchiorre, Mariana; Luca, Marcos Javier de; Anta, Gustavo González; Suarez, Paola; López, Carlos Fernández; Lascano, Ramiro; Racca, R. W.

doi:10.1007/s00374-010-0503-7

Cited by 35 publications

(35 citation statements)

References 18 publications

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“…However, several studies have reported variations in the morphological, physiological, genetic, and symbiotic characteristics of Bradyrhizobium isolated from soils inoculated with commercial inoculants, and they attribute these variations to genetic adaptation to native microorganisms and recombination processes with them (Ferreira and Hungria, 2002;Galli-Terasawa et al, 2003;Barcellos et al, 2007;Batista et al, 2007;Giongo et al, 2008). However, inoculant strains of B. japonicum or B. elkanii, cultured under laboratory conditions, also produced colonies with distinct morphology and genotypes (Sylvester-Bradley et al, 1988;Basit et al, 1991;Kober et al, 2004), and they can change the symbiotic relationships with the host plant (Fuhrmann, 1990;Melchiorre et al, 2011;Torres et al, 2012). According to the intensity of this variation, rhizobia may lose nodulation ability or become less efficient in N 2 fixation.…”

Section: Introductionmentioning

confidence: 99%

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Barbosa

Costa

Ribeiro

et al. 2017

Rev. Bras. Ciênc. Solo

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High yields obtained from soybean [Glycine max (L.) Merrill], and consequent profits, are related to efficient symbiosis of soybean with nitrogen fixing bacteria of the Bradyrhizobium genus. Inoculation with strains belonging to the Bradyrhizobium japonicum, B. elkanii, and B. diazofficiens species, approved by the Brazilian Ministry of Agriculture (MAPA), is a common practice for this crop. However, variations in the composition of inoculants can change the quality of the product. The aim of this study was to genetically identify and to evaluate the symbiotic efficiency of isolates with cultural characteristics different from those of strains approved by MAPA and which are present in two commercial inoculants for soybean. Isolates obtained from inoculants and the strains SEMIA 5079 (CPAC 15), SEMIA 5080 (CPAC 7), SEMIA 587 (BR 96), and SEMIA 5019 (BR 29), indicated in the formulation of these inoculants, were tested for symbiotic efficiency in soybeans (cultivar: COODETEC 5G 830 RR) in a greenhouse. Identification by partial sequencing of the 16S rRNA gene, together with BOX-PCR analysis, showed that variant isolates were not similar to the inoculant strains indicated in the commercial products, despite belonging to the same genus and/or species. No loss was observed in the nodulation ability of the isolates; however, significant differences were reported in the symbiotic efficiency of the isolates compared to the inoculant strains approved by MAPA. Nevertheless, the presence of morphological variants did not influence the efficiency of inoculant products.

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Section: Introductionmentioning

confidence: 99%

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Barbosa

Costa

Ribeiro

et al. 2017

Rev. Bras. Ciênc. Solo

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“…However, when populations of established rhizobia are already present, there may be competition between these and inoculated ones. In general, those already established no longer have the genotypes of the bacteria that are inoculated due to genetic alterations that occur (Melchiorre et al, 2011), eventually competing with the rhizobia that were inoculated and of better quality (López-García et al, 2009). Therefore, the NN may have been lower, but the bacteria that perform the symbiosis were more efficient in BNF.…”

Section: Resultsmentioning

confidence: 99%

Inoculation of soybean seeds coated with osmoprotector in diferents soil pH's

et al. 2018

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Inoculation with nitrogen-fixing bacteria (Bradyrhizobium) is an indispensable technology to increase the productivity of the soybean crop. The objective of this study was to evaluate the viability of the inoculation with Bradyrizobium bacteria associated with the osmoprotector coating on different presowing days and the effect of different soil pH on the components of yield, nodulation and grain yield of soybean. Two soybean cultivars were used, sown in two seasons and submitted to soil pH of 5.3 and 6.5. The seeds were inoculated at different pre-sowin periods with Bradyrizobium bacteria in the presence or absence of osmoprotectants. In the cultivar NA 5909RG (pH 5.3), the seeds inoculated with Bradyrhizobium and osmoprotector, at four and seven days of pre-sowing, presented an increase of 10.8% and 8.3% of productivity, in relation to those without osmoprotector. The osmoprotector and the inoculation of Bradyrhizobium increases the number and dry mass of nodules in soil with more acidic pH. The soil of lower acidity is responsible for higher grain yield, compared to the higher acidity, when both were not inoculated. The variable with the greatest explanatory power and contribution in the variability in the data of the experiment is the grain yield.

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“…A eficiência desses microorganismos tem possibilitado a obtenção de altos rendimentos de grãos da cultura sem a necessidade de aplicação de nitrogênio mineral (Alves et al, 2003;Zilli et al, 2006). O sucesso no processo de nodulação após a inoculação com rizóbio é determinado por vários fatores tais como fertilidade do solo e competitividade das estirpes dos inoculantes, avaliadas pela capacidade de sobreviver e colonizar as raízes das plantas em um ambiente com abundância de micro-organismos nativos produzindo nódulos de fixação (Melchiorre et al, 2011). Entre as estirpes de Bradyrhizobium selecionadas para a soja, a melhor capacidade de competir por sítios de nodulação no campo tem sido atribuída à espécie B. elkanii (Boddey & Hungria, 1997;Neves & Rumjanek, 1997) porém, quanto ao acúmulo de N, tem sido verificada maior eficiência promovida por B. japonicum (Neves & Rumjanek, 1996).…”

Section: Introductionunclassified

Interações entre fungos micorrízicos arbusculares, rizóbio e actinomicetos na rizosfera de soja

Pereira

Santos

Freitas

et al. 2013

Rev. bras. eng. agríc. ambient.

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RESUMOIsolados de actinomicetos e de fungos micorrízicos arbusculares foram avaliados em sistemas de inoculação conjunta in vivo, em plantas de soja inoculadas com rizóbios, com a finalidade de se observar interações microbianas. A pesquisa foi realizada no Centro Nacional de Pesquisa de Agrobiologia (Embrapa CNPAB). Foram utilizados, como substrato, solo da série Itaguaí (textura média) misturado com areia de rio na proporção 1:1 (v:v), com adição de isolados de actinomicetos (ACT-78 e ACT-370); estirpes de rizóbios (Bradyrhizobium elkanii -BR 29 e Bradyrhizobium japonicum -BR 33) e fungos micorrízicos arbusculares (Gigaspora margarita e Glomus clarum). Os resultados revelam que a inoculação com os fungos micorrízicos arbusculares influenciou de modo expressivo as variáveis de crescimento, nodulação e densidade de actinomicetos na rizosfera de soja, que apresentaram valores maiores do que as plantas sem o inóculo fúngico. Houve efeitos sinergísticos e antagônicos dos fungos micorrízicos arbusculares com os actinomicetos e com rizóbios. Palavras-chave: fixação biológica de nitrogênio, interações microbianas, sinergismo, antagonismoInteractions between arbuscular mycorrhizal fungi Rhizobium and actinomycetes in the rhizosphere of soybean ABSTRACT Actinomycete isolates and arbuscular mycorrhizal fungi were evaluated in vivo using jointly inoculation systems with soybean plants inoculated with rhizobia, with the aim to observe microbial interactions. The study was carried out at the National Center of Agrobiologic Research (Embrapa CNPAB). Soil of the Itaguai series (medium texture) was used as substrate mixed with river sand in proportion 1:1 (v:v), adding actinomycete isolates (ACT 78 and ACT 370), rhizobia strains (Bradyrhizobium elkanii -BR 29 and Bradyrhizobium japonicum -BR 33), and arbuscular mycorrhizal fungi (Gigaspora margarita and Glomus clarum). The results revealed the effect of the arbuscular mycorrhizal fungi addition with significant and expressive influence on growth parameters, nodulation and density of actinomycete isolates on the soybean rhizosphere which showed greater values compared with fungi uninoculated plants. Synergistic and antagonistic effects were observed between the arbuscular mycorrhizal fungi with actinomycetes isolates and rhizobia strains.

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Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants

Cited by 35 publications

References 18 publications

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Inoculation of soybean seeds coated with osmoprotector in diferents soil pH's

Interações entre fungos micorrízicos arbusculares, rizóbio e actinomicetos na rizosfera de soja

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