Evaluation of BMMSCs-EPCs sheets for repairing alveolar bone defects in ovariectomized rats

Abstract

The aim of this paper is to investigate the effect that bone marrow mesenchymal stem cells (BMMSCs) - endothelial progenitor cells (EPCs), BMMSCs and EPCs sheets have on repairing maxillary alveolar defects in ovariectomized (OVX) rats. In this study, after identification using multi-lineage differentiation and flow cytometry, BMMSCs and EPCs were isolated from female rats. The BMMSCs-EPCs, BMMSCs and EPCs sheets were detected by hematoxylin-eosin (H&E) staining, alkaline phosphatase (ALP) staining and qRT-PCR. Defects were created in maxillary alveoli and repaired with BMMSCs-EPCs, BMMSCs and EPCs sheets in OVX rats. The repair effects were determined by histological staining and micro-CT analysis at 2, 4 and 8 weeks after implantation. We aim to clarify whether BMMSCs-EPCs sheets are more effective in repairing alveolar bone defects than are BMMSCs and EPCs sheets in OVX rats. The results show that the osteogenic potential and the effect of bone repair are greater in the BMMSCs-EPCs sheet group and that this group has a higher ability to repair alveolar bone defects in OVX rats. These results suggest that BMMSCs-EPCs sheets have potential in clinical applications for treating humans with osteoporosis.

COL4A2 in the tissue-specific extracellular matrix plays important role on osteogenic differentiation of periodontal ligament stem cells

“…The multiple differentiation capacities of PDLSCs and BMSCs were determined according to the methods previously described 35,37. Briefly, 1 × 10 6 cells (P2) were cultured in α-MEM in 6-well plates without inducers until confluence.…”

“…Six immunocompromised mice were sorted into no-ECM + COL4A2 (TCP coated with COL4A2 peptide) and P-dECM + COL4A2 (P-dECM coated with COL4A2 peptide) implant transplantation groups. Briefly, we used a model of maxillary defects in rats as previously reported 37. A defect was prepared with a 3-mm-diameter round bur in the mesial-lingual side of the maxillary first molar in each SD rat, ensuring that the size of each defect was the same as that of the round bur.…”

“…Animal samples were fixed with 4% paraformaldehyde and the micro-CT scanning was reconstructed with an isotropic voxel size of 4 μm, and the three-dimensional images acquired from microtomographic slices were utilized for quantitative evaluation 37. For morphological and immunohistochemical analysis, tissue blocks were embedded in paraffin after decalcification in 10% EDTA (Sigma-Aldrich, 798681) for 4 weeks.…”

Biofunctionalized Scaffold in Bone Tissue Repair

“…MSCs are widely used in regenerative medicine; they are easily accessible and easy to expand and manipulate in vitro and when transplanted do not evoke a host immune-response [ 16 ]. Although the bone-marrow-derived MSCs are the most used for bone tissue engineering, other sources possess the same proliferation and differentiation abilities typical to be defined as mesenchymal [ 17 ]. Nowadays, new populations of MSCs from oral tissues, including periodontal ligament stem cells (PDLSCs), have been proposed as suitable MSC sources for bone tissue regeneration [ 18 ].…”

The Potential of Different Origin Stem Cells in Modulating Oral Bone Regeneration Processes

“…After 8 weeks post-transfection and in vivo implementation, in rats, the bone gaps were smaller and the tissue had a better developed angiogenic network [85]. The combination of BM-MSCs and endothelial progenitor cells (EPCs) induced more rapid bone formation in case of alveolar bone in vivo on ovariectomized rat [86].…”