Evaluation of Blueberry Juice in Mouse Azoxymethane‐Induced Aberrant Crypts and Oxidative Damage

Abstract

Blueberry is a plant with a number of nutritional and biomedical capabilities. In the present study we initially evaluated the capacity of its juice (BJ) to inhibit the number of aberrant crypts (AC) induced with azoxymethane (AOM) in mouse. BJ was administered daily by the oral route to three groups of animals during four weeks (1.6, 4.1, and 15.0 μL/g), respectively, while AOM (10 mg/kg) was intraperitoneally injected to the mentioned groups, twice a week, in weeks two and three of the assay. We also included two control groups of mice, one administered distilled water and the other the high dose of BJ. A significant increase of AC was observed in the AOM treated animals, and a mean protection of 75.6% was determined with the two low doses of BJ tested; however, the high dose of the juice administered together with AOM increased the number of crypts more than four times the value observed in animals administered only AOM. Furthermore, we determined the antioxidant potential of BJ with an ex vivo DPPH assay and found a dose-dependent decrease with a mean of 19.5%. We also determined the DNA oxidation/antioxidation by identifying 8-hydroxy-2′-deoxyguanosine adducts and found a mean decrease of 44.3% with the BJ administration with respect to the level induced by AOM. Our results show a complex differential effect of BJ related to the tested doses, opening the need to further evaluate a number of factors so as to determine the possibility of a cocarcinogenic potential.

Chemopreventive Effect of Cooked Chickpea on Colon Carcinogenesis Evolution in AOM/DSS-Induced Balb/c Mice

“…After washing with PBS pH 7.4 (4 °C), colon tissue was placed in a Petri dish, embedded with solid paraffin, and fixed with 4% formaldehyde in PBS (pH 7.4) for 24 h. Afterward, each tissue was stained with 4% methylene blue in PBS for 2 min. Subsequently, the number of crypts was identified and imaged with an optical microscope (Carl Zeiss, Primo Star) at 10X magnification [ 65 ].…”

Pro-oxidant activity of polyphenols and its implication on cancer chemoprevention and chemotherapy

“…Polyphenol-rich blueberry juice exerted a protective effect in AOM-induced ACF formation in mouse, and reduced the levels of oxidized DNA bases (8-hydroxy-2′-deoxyguanosine, 8-OHdG), and increased the antioxidant capacity to capture the 2,2-diphenil-1-picrilhydracil radical (DPPH) ex vivo [47].…”

Hypocholesterolemic and Anticarcinogenic Effect ofVicia fabaProtein Hydrolyzates

“…Even though this effect is yet to be confirmed in vivo, the suggestion may be valid in light of the fact that a number of reports have showed concordance between the in vitro and in vivo results. Furthermore, previous studies have demonstrated that besides forming colon ACF in mouse, AOM also induced in vivo oxidation to lipids, proteins, and DNA, and that chemopreventive agents act, at least partially, by blocking such oxidative damage (41,42,43).…”