Evaluation of biomarkers in  Mytilus galloprovincialis  as an integrated measure of biofilm-membrane bioreactor (BF-MBR) system efficiency in mitigating the impact of oily wastewater discharge to marine environment: a microcosm approach

Pirrone, Cristina; Rossi, Federica; Cappello, Simone; Borgese, Marina; Mancini, Giuseppe; Bernardini, Giovanni; Gornati, Rosalba

doi:10.1016/j.aquatox.2018.02.018

Cited by 11 publications

(7 citation statements)

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“…The two affected clam populations were characterized by the presence of haemocyte infiltration and epithelial cell degradation during winter and autumn. This phenomenon has been well described as one of the responses to diverse environmental stressors (Pirrone et al 2018). It has previously been established that haemocyte infiltration is the cause of the inflammatory process due to a rapid release of pro-inflam-matory and vasoactive mediators (Bouallegui et al 2018).…”

Section: Discussionmentioning

confidence: 98%

Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons

Bejaoui

Rabeh

Telahigue

et al. 2020

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The aim of the present study was to investigate the combined effects of seasonality and anthropogenic pressure on a battery of oxidative stress, lipid peroxidation, protein oxidation, DNA damage and histological alterations in the native clam Ruditapes decussatus collected from a less contaminated area (LCA), Ghar El Melh, a moderately contaminated area (MCA), the North Lake, and a highly contaminated area (HCA), the South Lake, all located in the southern Mediterranean Sea. The accumulation of cadmium, lead, copper, iron and zinc was higher in the digestive glandsof clams collected from the MCA and the HCAthan in those from the LCA, particularly during the warm season. Our results reveal that metallothionein, lipid peroxidation, protein oxidation levels and antioxidant defence systems were higher, while acetylcholinesterase activity was lower, in clams from the MCAand HCAthan in those from the LCA. The results also indicate that clams from the MCA and the HCAare characterized by histological alterations and DNA damage. In conclusion, the evident changes of antioxidant defence systems and macromolecules between the studied lagoons reveal the perturbation of the physiological states of clams from polluted sites thatcope with seasonal changes and trace element accumulations.

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Section: Discussionmentioning

confidence: 98%

Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons

Bejaoui

Rabeh

Telahigue

et al. 2020

scimar

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“…to naphthenic acids. Microcosms are ideal for use in situations where environmental control and experimental repeatability are required while maintaining specific environmental conditions ( Pirrone et al, 2018 ). Moreover, the use of microcosms is well established for toxicity testing, owing primarily to the increased transferability of results between field and laboratory environments as microcosms can closely mimic those of target ecosystems ( Alexander et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Metabolomic Analysis of Hexagenid Mayflies Exposed to Sublethal Concentrations of Naphthenic Acid

Pomfret

Brua

Milani

et al. 2021

Front. Mol. Biosci.

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The oil sands region in northeastern Alberta, Canada contain approximately 165 billion barrels of oil making it the third largest oil reserves in the world. However, processing of extracted bitumen generates vast amounts of toxic byproduct known as oil sands process waters. Naphthenic acids and associated sodium naphthenate salts are considered the primary toxic component of oil sands process waters. Although a significant body of work has been conducted on naphthenic acid toxicity at levels comparable to what is observed in current oil sands process waters, it is also important to understand any impacts of exposure to sublethal concentrations. We conducted a microcosm study using the mayfly Hexagenia spp. to identify sublethal impacts of naphthenic acid exposure on the survival, growth, and metabolome across a concentration gradient (0–100 μg L−1) of sodium naphthenate. Nuclear magnetic resonance-based metabolomic analyses were completed on both the polar and lipophilic extracted fractions of whole organism tissue. We observed a positive relationship between sodium naphthenate concentration and mean principal component score of the first axis of the polar metabolome indicating a shift in the metabolome with increasing naphthenic acid exposure. Eleven metabolites correlated with increased naphthenic acid concentration and included those involved in energy metabolism and apoptosis regulation. Survival and growth were both high and did not differ among concentrations, with the exception of a slight increase in mortality observed at the highest concentration. Although lethal concentrations of naphthenic acids in other studies are higher (150–56,200 μg L−1), our findings suggest that physiological changes in aquatic invertebrates may begin at substantially lower concentrations. These results have important implications for the release of naphthenic acids into surface waters in the Alberta oil sands region as an addition of even small volumes of oil sands process waters could initiate chronic effects in aquatic organisms. Results of this research will assist in the determination of appropriate discharge thresholds should oil sands process waters be considered for environmental release.

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“…For microscopy, about 800 μL of rehydrated FWM, containing 10,000 cells, were placed in a 24-well plate and grown for 24, 48, 72 or 96 h, and then processed as reported in Pirrone [ 19 ]. Briefly, samples were fixed in Karnovsky solution (2% glutaraldehyde, 4% formaldehyde in 0.1 M sodium cacodylate buffer, pH 7.4) for 24 h at 4 °C and then preserved in 0.1 M sodium cacodylate buffer at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…For microscopy, about 800 μL of rehydrated FWM, containing 10,000 cells, were placed in a 24well plate and grown for 24, 48, 72 or 96 h, and then processed as reported in Pirrone [19]. Briefly,…”

Section: Optical and Electron Microscopymentioning

confidence: 99%

“…For microscopy, about 800 µL of rehydrated FWM, containing 10,000 cells, were placed in a 24-well plate and grown for 24, 48, 72 or 96 h, and then processed as reported in Pirrone [19]. Briefly, samples were fixed in Karnovsky solution (2% glutaraldehyde, 4% formaldehyde in 0.1 M sodium cacodylate buffer, pH 7.4) for 24 h at 4 • C and then preserved in 0.1 M sodium cacodylate buffer at 4 • C. Specimens were post-fixed using 1% OsO 4 in 0.1 M sodium cacodylate buffer (pH 7.4), dehydrated in ethanol (50%, 70%, 90% and 100%) and embedded in Epon-Araldite 812 as described in Bava [20].…”

Section: Optical and Electron Microscopymentioning

confidence: 99%

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Effect of Nanostructured Scaffold on Human Adipose-Derived Stem Cells: Outcome of In Vitro Experiments

et al. 2020

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This work is addressed to provide, by in vitro experiments, results on the repercussion that a nanostructured scaffold could have on viability, differentiation and secretion of bioactive factors of human adipose-derived stem cells (hASCs) when used in association to promote angiogenesis, a crucial condition to favour tissue regeneration. To achieve this aim, we evaluated cell viability and morphology by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and microscopy analysis, respectively. We also investigated the expression of some of those genes involved in angiogenesis and differentiation processes utilizing quantitative polymerase chain reaction (qPCR), whereas the amounts of Vascular Endothelial Growth Factor A, Interleukin 6 and Fatty Acid-Binding Protein 4 secreted in the culture medium, were quantified by enzyme-linked immunosorbent assay (ELISA). Results suggested that, in the presence of the scaffold, cell proliferation and the exocytosis of factors involved in the angiogenesis process are reduced; by contrast, the expression of those genes involved in hASC differentiation appeared enhanced. To guarantee cell survival, the construct dimensions are, generally, smaller than clinically required. Furthermore, being the paracrine event the primary mechanism exerting the beneficial effects on injured tissues, the use of conditioned culture medium instead of cells may be convenient.

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Evaluation of biomarkers in Mytilus galloprovincialis as an integrated measure of biofilm-membrane bioreactor (BF-MBR) system efficiency in mitigating the impact of oily wastewater discharge to marine environment: a microcosm approach

Cited by 11 publications

References 43 publications

Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons

Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons

Metabolomic Analysis of Hexagenid Mayflies Exposed to Sublethal Concentrations of Naphthenic Acid

Effect of Nanostructured Scaffold on Human Adipose-Derived Stem Cells: Outcome of In Vitro Experiments

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