Evaluation of biologically active secondary metabolites isolated from the toothache plant Acmella ciliata (Asteraceae)

Sharma, Rahul; Karunambigai, A.; Gupta, Somlee; Arumugam, N.

doi:10.1007/s13596-021-00584-5

Cited by 8 publications

(8 citation statements)

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“…The ethanolic and water-soluble extracts of A. montana flowers had IC 50 values of 0.66 and 1.71 mg/mL respectively, for antioxidant activity [60]. The flower methanolic extract of Acmella ciliate (Asteraceae) showed a positive correlation between antioxidant activity and total phenolic and flavonoid contents, which has also been reported in other species [61]. The fact that antioxidant activity was greatly increased in the three fractions by comparison to the complete methanolic extract of cell-dried biomass of A. montana (almost 50-to 150-fold), which was even higher than that reported in flower extracts [60], highlights the potential of a cell culture of A. montana as a source of antioxidant compounds, and the importance of performing fractionation that allows for concentrated SMs, thus improving bioactivities.…”

Section: Discussionmentioning

confidence: 77%

“…species [61]. The fact that antioxidant activity was greatly increased in the three fractions by comparison to the complete methanolic extract of cell-dried biomass of A. montana (almost 50-to 150-fold), which was even higher than that reported in flower extracts [60], highlights the potential of a cell culture of A. montana as a source of antioxidant compounds, and the importance of performing fractionation that allows for concentrated SMs, thus improving bioactivities.…”

Section: Discussionmentioning

confidence: 92%

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Arnica montana Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, α-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Nieto-Trujillo

Cruz-Sosa

Lurı́a-Pérez

et al. 2021

Plants

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Arnica montana cell suspension culture could be a sustainable source of a vegetal material producer of secondary metabolites (SMs) possessing biological effects. Different plant growth regulator concentrations (0–5 mg/L) were tested in foliar explants to induce a callus that was used to establish a cell suspension culture. Growth kinetics was carried out for 30 days. A methanolic extract obtained from biomass harvested at 30 days of growth kinetics was fractionated, and three fractions were tested for bioactivities. We induced a callus with 1 mg/L of picloram and 0.5 mg/L of kinetin in foliar explants, which allowed for the establishment of a cell suspension culture, and the latter had the highest total SMs contents at day 30. Three fractions showed differences in total SMs contents, with the highest values per gram as follows: 270 mg gallic acid equivalent for total phenolic content, 200 mg quercetin equivalent for total flavonoid content, 83 mg verbascoside equivalent for total phenolic acid content, and 396 mg parthenolide equivalent for total sesquiterpene lactone content. The best bioactivities were 2–6 µg/mL for the 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical, 30% cellular viability of lymphoma cells at 40 µg/mL, 17% inhibition against Escherichia coli and Staphylococcus aureus at 8 µg/disk, and α-amylase inhibition at 12% with 10 µg/mL. The total SMs contents were correlated with bioactivities.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 92%

Arnica montana Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, α-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Nieto-Trujillo

Cruz-Sosa

Lurı́a-Pérez

et al. 2021

Plants

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“…The validity of the method was con rmed following International Conference on Harmonization (ICH, 1997). The limit of detection (LOD), limit of quanti cation (LOQ) and precision (expressed as percent relative standard deviation, RSD%) of the HPLC method were calculated as described earlier (Sharma et al 2021).…”

Section: Estimation Of N-alkylamides and Methods Validationmentioning

confidence: 99%

Isolation and Characterization of Larvicidal N-alkylamides from Acmella ciliata HBK

Arumugam

Sharma

2022

Preprint

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The transmission of pathogens by mosquito vectors predisposes several diseases leading to severe human mortality worldwide. The phenomenon of acquired resistance to insecticides or larvicides intensifies a thrust for discovery of novel alternatives for control of the diseases spread by these vectors. Present study demonstrates isolation of a couple of major N-alkylamides from A. ciliata and reveal larvicidal activity (LA) on larvae of Aedes aegypti and Culex quinquefasciatus that spread pathogens of dengue and filariasis respectively. The two compounds were identified to be 2E,4E-N-isobutyl undeca-2,4-diene-8,10-diynamide (NUD) and 2E,4E,8Z,10E-N-isobutyl dodeca-2,4,8,10-tetraenamide (NDT). The LC50 of NUD for late III – early IV instar larvae of Ae. aegypti was 44.19 ppm and 30.89 ppm respectively. The LC50 of NDT for the same were 18.28 ppm and 11.75 ppm respectively. NDT was found more toxic than NUD with both having a higher potency than methoprene (MET), the commercial larvicide. Quantitative structure-activity relationship (QSAR) study revealed that the chain length, degree of unsaturation and terminal methylation in the molecules influence the extent of LA. The compounds isolated in this study were further demonstrated for use as standards in analytical biochemistry. NUD and NDT content varied, in different parts of the plant, with both accumulating maximally at 19.01 mg/g and 68.55 mg/g, respectively in flowers. The LOD and LOQ for estimation of NUD by HPLC was 0.80 µg/ml and 2.51 µg/ml respectively, whereas, these were 5.50 µg/ml and 16.70 µg/ml, respectively, for NDT. To the best of our knowledge, this is the first report on the purification of larvicidal N-alkylamides from A. ciliata and demonstration of their use as biochemical standard.

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“…17,18 For example, it has been reported that sesquiterpene lactones are the major compounds in Arnica montana and Bellis perennis flowers, while for Calendula officinalis they are sesquiterpene lactones, pentacyclic triterpenes, flavonoids, and phenolic acids. 17 For the flowers Acmella ciliata, 19 Cepris capillaris, Hieracium pilosella, and Hypochaeris radicata, a diverse composition of flavonoids and phenolic acids has been reported. 20,21 There is little information on the phytochemical profile of Dahlia; however, a study conducted on D. pinnata, whose extraction was performed using water and methanol (polar solvents), revealed the presence of flavonoids, saponins, and tannins and the absence of reducing sugars and steroids.…”

Section: Phytochemical Profile By Colorimetric Assaysmentioning

confidence: 99%

Phytochemical profile of petals from black Dahlia pinnata by flow injection analysis–electrospray ionization–Fourier transform ion cyclotron resonance mass spectrometry

Granados‐Balbuena,

Díaz‐Pacheco,

García‐Meza

et al. 2023

Phytochemical Analysis

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IntroductionDahlia pinnata Cav. is a flower native to Mexico that has many applications; in particular, its petals have been used for ornamental, food, and medicinal purposes, for example to treat skin rashes and skin cracks. It has been reported that the medicinal properties of plants are generally related to the phytochemical constituents they possess. However, there are few studies on black D. pinnata.ObjectivesThe present study was aimed at qualitatively and quantitatively determining the phytochemical profile of petals from black D. pinnata.MethodologyPhytochemicals from Dahlia petals were extracted by consecutive maceration (hexane, dichloromethane, and methanol); then, the extracts were analyzed through colorimetric assays and UV–Vis spectroscopy for qualitative identification and quantification of phytochemical compounds, respectively. The methanolic extract was analyzed by flow injection analysis–electrospray ionization–Fourier transform ion cyclotron resonance mass spectrometry (FIA‐ESI‐FTICR‐MS) in negative and positive mode.ResultsQuantitative phytochemical profiling of the methanolic extract by UV–Vis spectroscopy indicated high contents of phenolic compounds (34.35 ± 3.59 mg EQ/g plant) and sugars (23.91 ± 1.99 mg EQ/g plant), while the qualitative profiling by FIA‐ESI‐FTICR‐MS allowed the tentative identification of several flavonoids and phenolic acids. Kaempferol‐3‐rutinoside, pelargonidin‐3‐(6″‐malonylglucoside)‐5‐glucoside, rutin, kaempferol‐3‐(2″,3″‐diacetyl‐4″‐p‐coumaroylrhamnoside), and myricetin‐3‐(2‴‐galloylrhamnoside) were the main compounds detected.ConclusionThe results expand our knowledge of the phytochemical constituents of petals from black D. pinnata.

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Evaluation of biologically active secondary metabolites isolated from the toothache plant Acmella ciliata (Asteraceae)

Cited by 8 publications

References 41 publications

Arnica montana Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, α-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Arnica montana Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, α-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Isolation and Characterization of Larvicidal N-alkylamides from Acmella ciliata HBK

Phytochemical profile of petals from black Dahlia pinnata by flow injection analysis–electrospray ionization–Fourier transform ion cyclotron resonance mass spectrometry

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