Evaluation of Biological Properties of New Selective Differential Medium for Cholera Vibrios Isolation Based on the Results of Laboratоry Trials

Mazrukho, A B; Shelokhovich, A. I.; Harabadzhakhian, G. D.; Karbyshev, G. L.; Терентьев, А Н; Кругликов, В. Д.; Григоренко, Л. В.; Agaphonova, V. V.; Savel`eva, I. K.; Simakova, D. I.; Сокиркина, О Г; Bulakhova, O. G.; Egorenkova, I. S.

doi:10.21055/0370-1069-2011-2(108)-64-67

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“…Имеются данные литературы об использовании комплекса диагностических питательных сред на основе гидролизата пекарских дрожжей для выделения и накопления холерных вибрионов [5,7,8]. Однако в доступной нам литературе нет сведений о возможности применения сред на основе автолизата или гидролизата дрожжей при глубинном культивировании штаммов, используемых в производстве ХВ.…”

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Development of Food-Raw-Material-Based Nutrient Media for Submerged Cultivation of Cholera Vibrio Strains

Никифоров¹,

Антонычева²,

Volokh³

et al. 2015

Problemy Osobo Opasnykh Infektsii

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Carried out has been comparative assessment between the performances of liquid nutrient pancreatic fibrin overcook-based and bakery yeast autolyzate-based media and conventionally used in manufacturing of cholera vaccine media for submerged cultivation of Vibrio cholerae 569B and V. cholerae M-41 strains. Results of investigation of media quality biological predictors (morphological and biochemical property stability, efficacy of biomass and protective antigen accumulation), which are fibrin hydrolyzate and bakery yeast autolyzate-based, suggest the possibility of using them for the production of cholera vaccine. Deployment of inedible raw material-based media in manufacturing of cholera vaccine is a prospective technology in view of reduction of medical-prophylactic preparation costs. Moreover it allows for solving the problem of protein waste-product disposal, which is generated in the process of anti-rabies immunoglobulin manufacturing, thus decreasing ecological impact on the environment.

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