Evaluation of biological pretreatments to increase the efficiency of the saccharification process using Spartina argentinensis as a biomass resource

Larran, Alvaro Santiago; Jozami, Emiliano; Vicario, Lionel R.; Feldman, Susana Raquel; Podestá, Florencio E.; Permingeat, Hugo R.

doi:10.1016/j.biortech.2015.06.150

Cited by 29 publications

(11 citation statements)

References 22 publications

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“…yielded the highest enzymatic activities of endoglucanase and beta-glucosidases [ 108 ]. In Spratina argentiniensis , different pre-treatments (phosphoric acid, ligninolytic enzymes and fungal supernatants) aimed to remove lignin and improving cellulose hydrolysis efficiency were assessed [ 109 ]. Results show that pretreatment with Pycnoporus sanguineus supernatant improved fermentable carbohydrates availability, yielding 56.84% cellulose hydrolysis.…”

Section: Halophytes As Potential Sources Of Biodiesel and Bioethanolmentioning

confidence: 99%

Facing the challenge of sustainable bioenergy production: Could halophytes be part of the solution?

et al. 2017

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Due to steadily growing population and economic transitions in the more populous countries, renewable sources of energy are needed more than ever. Plant biomass as a raw source of bioenergy and biofuel products may meet the demand for sustainable energy; however, such plants typically compete with food crops, which should not be wasted for producing energy and chemicals. Second-generation or advanced biofuels that are based on renewable and nonedible biomass resources are processed to produce cellulosic ethanol, which could be further used for producing energy, but also bio-based chemicals including higher alcohols, organic acids, and bulk chemicals. Halophytes do not compete with conventional crops for arable areas and freshwater resources, since they grow naturally in saline ecosystems, mostly in semi-arid and arid areas. Using halophytes for biofuel production may provide a mid-term economically feasible and environmentally sustainable solution to producing bioenergy, contributing, at the same time, to making saline areaswhich have been considered unproductive for a long time -more valuable. This review emphasises on halophyte definition, global distribution, and environmental requirements. It also examines their enzymatic valorization, focusing on salt-tolerant enzymes from halophilic microbial species that may be deployed with greater advantage compared to their conventional mesophilic counterparts for faster degradation of halophyte biomass.

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Section: Halophytes As Potential Sources Of Biodiesel and Bioethanolmentioning

confidence: 99%

Facing the challenge of sustainable bioenergy production: Could halophytes be part of the solution?

et al. 2017

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“…The commonly used pretreatment methods for LC biomass include acid treatment, alkaline treatment, ammonia fiber expansion, organosolv treatment, liquid hot water treatment, microwave irradiation, superheated steam treatment, steam explosion and microbial pretreatment. Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase are the key enzymes in lignin degradation . Among these enzymes, LiP can oxidize and degrade lignin without metal ions in the presence of H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase are the key enzymes in lignin degradation. 12 Among these enzymes, LiP can oxidize and degrade lignin without metal ions in the presence of H 2 O 2 . 13,14 The oxidative degradation of lignin catalyzed by enzymes has some advantages over chemical degradation, such as destruction of the crystal structure of cellulose and short pretreatment cycle.…”

Section: Introductionmentioning

confidence: 99%

The synergistic effect of lignin peroxidase and cellulase in Aspergillus oryzae solid‐state fermentation substrate on enzyme‐catalyzed oxidative degradation of lignin

Zhang

Wang

Fan

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND We have reported the oxidative degradation of lignin catalyzed by lignin peroxidase (LiP) from Aspergillus oxyzae broth in previous studies. The aim of the present paper is to obtain the maximal yield of total reducing sugar (Ytrs) by synergistic effect analysis between LiP and three components of cellulase (endo‐β‐1,4‐glucanase, exo‐β‐1,4‐glucanase and β‐glucosidase). RESULTS The Michaelis–Menten analogous model was applied to fit experimental data of lignin oxidative degradation. The apparent Michaelis constant of purified LiP product (3.02 mg mL–1) was lower than that of the crude LiP solution (4.74 mg mL−1), and the apparent maximum reaction rate of the purified LiP product (1.15 mg mL−1 h−1) was higher than that of the crude LiP solution (0.86 mg mL−1 h−1). The maximum Ytrs (49.794%) was obtained when the β‐glucosidase activity was 0; the activities of LiP, endo‐β‐1,4‐glucanase and exo‐β‐1,4‐glucanase were 10.77, 3.360 and 3.427 U mL−1 (3:1:1), respectively. The components and structure of the substrate was extremely different before and after synergistic action under optimal conditions. CONCLUSION A synergistic effect exists between LiP and cellulase. β‐glucosidase is the key enzyme that inhibits the oxidative degradation of lignin. The results lay the foundation for screening and optimizing fermentation process conditions of the LiP strain, and improving the efficiency of enzyme‐catalyzed oxidative degradation of lignin. © 2018 Society of Chemical Industry

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“…Thus, the strategy for utilizing lignin as sustainable feedstock for producing value-added phenolic derivatives is not still enough yet [ 8 ]. Even though various chemical pretreatments have been attempted in biorefinery, recently ligninolytic biocatalysts for pretreatment are emerging due to their superior selectivity, lower energy consumption, and unparalleled sustainability [ 9 , 10 ]. In nature, heme-containing peroxidases such as lignin peroxidase (LiP, E.C.…”

Section: Introductionmentioning

confidence: 99%

Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase

Min

Yum

Kim

et al. 2017

Biotechnol Biofuels

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BackgroundIn the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date.ResultsHerein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and Cα–Cβ bonds in 4-phenoxyphenol and guaiacyl glycerol-β-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates.ConclusionThese results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0900-3) contains supplementary material, which is available to authorized users.

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Evaluation of biological pretreatments to increase the efficiency of the saccharification process using Spartina argentinensis as a biomass resource

Cited by 29 publications

References 22 publications

Facing the challenge of sustainable bioenergy production: Could halophytes be part of the solution?

Facing the challenge of sustainable bioenergy production: Could halophytes be part of the solution?

The synergistic effect of lignin peroxidase and cellulase in Aspergillus oryzae solid‐state fermentation substrate on enzyme‐catalyzed oxidative degradation of lignin

Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase

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