Evaluation of Biological Effects and Possible Mechanisms of Static Magnetic Field Action

Чехун, В Ф; Demash, Dmytro V.; Naleskina, L A

doi:10.1615/intjphyspathophys.v4.i1.80

Cited by 7 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are numerous studies and hypotheses that suggest the membrane represents the primary site of interaction (Chekhun et al, 2013;Junji, 2006). Fluidity (Lin et al, 2008), ion channel (Rosen, 2003) and mechanical properties (Wang et al, 2014) of membrane are considered to be the principle targets of SMF action.…”

Section: Discussionmentioning

confidence: 99%

Regulation of osteoclast differentiation by static magnetic fields

Zhang

Meng

Ding

et al. 2016

Electromagnetic Biology and Medicine

View full text Add to dashboard Cite

Static magnetic field (SMF) modulates bone metabolism, but little research is concerned with the effects of SMF on osteoclast. Our previous studies show that osteogenic differentiation is strongly correlated with magnetic strength from hypo (500 nT), weak (geomagnetic field, GMF), moderate (0.2 T) to high (16 T) SMFs. We speculated that the intensity that had positive (16 T) or negative (500 nT and 0.2 T) effects on osteoblast differentiation would inversely influence osteoclast differentiation. To answer this question, we examined the profound effects of SMFs on osteoclast differentiation from pre-osteoclast Raw264.7 cells. Here, we demonstrated that 500 nT and 0.2 T SMFs promoted osteoclast differentiation, formation and resorption, while 16 T had an inhibitory effect. Almost all the osteoclastogenic genes were highly expressed under 500 nT and 0.2 T, including RANK, matrix metalloproteinase 9 (MMP9), V-ATPase, carbonic anhydrase II (Car2) and cathepsin K (CTSK), whereas they were decreased under 16 T. In addition, 16 T disrupted actin formation with remarkably decreased integrin β3 expression. Collectively, these results indicate that osteoclast differentiation could be regulated by altering the intensity of SMF, which is just contrary to that on osteoblast differentiation. Therefore, studies of SMF effects could reveal some parameters that could be used as a physical therapy for various bone disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Regulation of osteoclast differentiation by static magnetic fields

Zhang

Meng

Ding

et al. 2016

Electromagnetic Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Genes related to cell stress defense mechanisms, including genes encoding molecular chaperones, antioxidant and pro-oxidant enzymes, and proteins involved in xenobiotic metabolism, may also be induced under free radical oxidative stress (Fulda et al 2010 ). Based on the available data, Chekhun et al ( 2012 ) concluded that the exposure of cells to SMF may cause disruption of free radical metabolism and the elevation of their concentration. In turn, free radical damage can be controlled by the suitable antioxidant defense systems.…”

Section: Discussionmentioning

confidence: 99%

Effect of static magnetic fields and phloretin on antioxidant defense system of human fibroblasts

Pawłowska-Góral

Kimsa-Dudek

Synowiec-Wojtarowicz

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

The available evidence from in vitro and in vivo studies is deemed not sufficient to draw conclusions about the potential health effects of static magnetic field (SMF) exposure. Therefore, the aim of the present study was to determine the influence of static magnetic fields and phloretin on the redox homeostasis of human dermal fibroblasts. Control fibroblasts and fibroblasts treated with phloretin were subjected to the influence of static magnetic fields. Three chambers with static magnetic fields of different intensities (0.4, 0.55, and 0.7 T) were used in the study. Quantification of superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), glutathione peroxidase 1 (GPX1), microsomal glutathione S-transferase 1 (MGST1), glutathione reductase (GSR), and catalase (CAT) messenger RNAs (mRNAs) was performed by means of real-time reverse transcription PCR (QRT-PCR) technique. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities were measured using a commercially available kit. No significant differences were found in SOD1, SOD2, GPX1, MGST1, GSR, and CAT mRNA levels among the studied groups in comparison to the control culture without phloretin and without the magnet. There were also no changes in SOD, GPx, and CAT activities. In conclusion, our study indicated that static magnetic fields generated by permanent magnets do not exert a negative influence on the oxidative status of human dermal fibroblasts. Based on these studies, it may also be concluded that phloretin does not increase its antioxidant properties under the influence of static magnetic fields. However, SMF-induced modifications at the cellular and molecular level require further clarification.

show abstract

“…Additionally, Static Magnetic Field (SMF) has a wide range of biological effects as a physical factor 3) . Studies have demonstrated that SMF could promote bone repairing and formation 4,5) . On cellular level, SMF has been showed that it could promote human osteoblastic differentiation 6) .…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Study of the Effect of Static Magnetic Field Acting on Rat Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation In Vitro

Chuo

Ma²,

Saito

et al. 2013

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Bone marrow mesenchymal stem cells (BMMSCs) could be induced to osteoblasts, which were considered as the seed cells source for organizational engineering. Moreover, it has been demonstrated that Static Magnetic Field (SMF) can stimulate osteoblasts differentiation, but SMF stimulation acting on BMMSCs remained unknown results. The aim of this study is to investigate the effect of SMF acting on rat BMMSCs during osteogenic differentiation in vitro. The experimental BMMSCs were divided into 2 groups, which were experiment group and control group, and both of them were induced by differentiate medium. Experiment group was exposed to SMF at 200 mT 24h/day during osteogenesis. Contrarily, control group was not exposed to SMF during osteogenesis. Results showed that SMF exposure significantly increased the cell proliferation rate, alkaline phosphatase (ALP) activity, as well as the expression of osteogenesis-related proteins. Besides, matrix mineralization was significantly more visible in experiment group than that in control group on the 7th day of the experimentation. In summary, SMF exposure could significantly enhance osteogenic differentiation and mineralization of rat BMMSCs.

show abstract

Evaluation of Biological Effects and Possible Mechanisms of Static Magnetic Field Action

Cited by 7 publications

References 0 publications

Regulation of osteoclast differentiation by static magnetic fields

Regulation of osteoclast differentiation by static magnetic fields

Effect of static magnetic fields and phloretin on antioxidant defense system of human fibroblasts

A Preliminary Study of the Effect of Static Magnetic Field Acting on Rat Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation In Vitro

Contact Info

Product

Resources

About