Evaluation of biologic effects of dental materials using four different cell culture techniques

Abstract

– The cytotoxicity of fresh and 1‐day‐old silicate cement, composite restorative material and zinc oxide‐eugenol cement (ZOE) was tested using human epithelial cells (NCTC 2544) in four different cell culture systems: (1) 5.1Cr‐release from prelabeled cells after incubation for 4 and 24 h in the presence of the materials, (2) Implanting the materials on an agar overlay and visualizing any cytotoxic effects after 24 h by neutral red vital stain. (3) Cell growth during 5 d in the presence of the materials7hellip; (4) Colony‐forming ability after exposure of the cells for 30 min to medium previously incubated with the materials for 24 h. Freshly prepared and 1‐day‐old ZOE exhibited a prominent cytotoxic effect in all four systems. A less marked effect was found for the composite material in systems 2, 3, and 4, while silicate cement appeared to be the least toxic material in these three systems. Neither silicate cement nor composite showed any cytotoxic effect in system 1 based on51Cr‐release. It is concluded that the effects obtained by the cell culture techniques did not mimic the reactions obtained when the materials are tested under conditions which reflect their clinical use.

New developments in the filter test system for cytotoxicity testing

“…This is in contrast to findings by Hensten-Pettersen and Helgeland [29] who reported little agreement between different cell culture methods. However, these authors used completely different test systems with different cell lines and different elution conditions.…”

51Cr release cytotoxicity assay evaluated with fluoride and cadmium

“…The 51Cr release assay is applied in tests for cytotoxicity [1][2][3][4], immunologically induced cell damage [5][6] and for determining the life span of erythrocytes in vivo [7].…”

Bioavailability of Components of Resin-Based Materials Which Are Applied To Teeth

“…Whole resin composites, when placed into culture medium with cells, caused a variety of cytotoxic effects (Spangberg et al, 1973;Hensten-Pettersen and Helgeland, 1977;Tyas and Browne, 1977;Hanks et al, 1981;Imai and Nakamura, 1989;Kaga etal, 1989;Meryon and Brook, 1989). Jontell et al (1995) described the cytotoxic effects of several resins on lymphocytes and also noted stimulation of lymphocyte proliferation at low concentrations, which may be significant in possible immune responses.…”