Evaluation of bioaerosol sampling techniques for the detection of Chlamydophila psittaci in contaminated air

Droogenbroeck, Caroline Van; Risseghem, Marleen Van; Braeckman, Lutgart; Vanrompay, Daisy

doi:10.1016/j.vetmic.2008.09.042

Cited by 35 publications

(32 citation statements)

References 33 publications

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“…During the present study we also evaluated the practical application of a personal and stationary bioaerosol monitoring technique especially designed for entrapping C. psittaci DNA as well as live organisms (19). The stationary bioaerosol sampling method proved to be of high value for quantifying the chlamydial organisms in the air and allowed us to determine the zoonotic risk in both slaughterhouses.…”

Section: Discussionmentioning

confidence: 99%

“…Personal monitoring was performed at the same locations except the plucking room, as the plucking machine operated fully automatically. Stationary bioaerosol monitoring was performed using an MAS-100 ecosampler (Merck, Darmstadt, Germany), together with in-house-made semisolid collection medium, ChlamyTrap (19), at an airflow rate of 100 liters/ min for 10 min. Personal bioaerosol monitoring was performed by use of an IOM personal inhalable dust sampler (SKC Inc., Eighty Four, PA) provided with a gelatin filter (3-m pore size; SKC) at an airflow rate of 2 liters/min for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…Incoming flocks as well as employees were sampled. Additionally, C. psittaci bioaerosol monitoring was performed using a recently developed personal and stationary (19) bioaerosol monitoring technique allowing the detection of chlamydial DNA as well as live organisms. The aim was to determine the zoonotic risk at different times and locations in the slaughterhouses.…”

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confidence: 99%

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Chlamydophila psittaci Zoonotic Risk Assessment in a Chicken and Turkey Slaughterhouse

Dickx

Geens²,

Deschuyffeleer

et al. 2010

J Clin Microbiol

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Chlamydophila psittaci causes respiratory disease in poultry and can be transmitted to humans. We conducted a C. psittaci zoonotic risk assessment study of a chicken and turkey slaughterhouse. Eighty-five percent of the slaughtered chicken flocks tested positive by PCR and culture. Genotype D was discovered. Fifty-seven percent of the slaughtered turkey flocks tested positive by PCR and culture. Genotype D was present. For the chicken slaughterhouse employees, 7.5% and 6% tested positive for C. psittaci by PCR and culture, respectively. In the turkey slaughterhouse, 87% and 61% of the employees tested positive by PCR and culture, respectively. All genotyped human samples contained genotype D. Using stationary bioaerosol monitoring by means of an MAS-100 ecosampler and ChlamyTrap collection medium, chlamydial DNA, and viable organisms were detected in both the chicken and turkey slaughterhouses. Positive air samples were most frequently found in the animal reception area and evisceration room. Zoonotic transmissions were very common, especially from processed turkeys. Accurate diagnostic monitoring and reporting of C. psittaci infections should be promoted in poultry workers.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Chlamydophila psittaci Zoonotic Risk Assessment in a Chicken and Turkey Slaughterhouse

Dickx

Geens²,

Deschuyffeleer

et al. 2010

J Clin Microbiol

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“…Two subsequent air samples were taken at several time points during this hatching process: (i) in the morning when only a few animals (10 %) had hatched (T1); (ii) at noon (T2); and (iii) in the evening when most animals (90 %) had hatched (T3). Bioaerosol monitoring was performed using the MAS-100 Eco Sampler (Merck) together with the in-house-designed air collection medium ChlamyTrap at an air flow rate of 100 l min 21 for 10 min (Van Droogenbroeck et al, 2009a). At each time point, two samples were taken.…”

Section: Methodsmentioning

confidence: 99%

Zoonotic transmission of Chlamydia psittaci in a chicken and turkey hatchery

Dickx

Vanrompay

2011

Journal of Medical Microbiology

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Chlamydia psittaci is an obligately intracellular Gram-negative bacterium causing respiratory disease (chlamydiosis) or asymptomatic carriage in birds. C. psittaci is a zoonotic agent causing psittacosis or parrot fever in humans. Vertical and/or horizontal transmission via eggs might have serious repercussions on the C. psittaci infection status of poultry flocks and thus on zoonotic risk for all workers along the poultry supply chain. We therefore studied the presence of C. psittaci in a hatchery. In addition, we examined all (n=4) employees of the hatchery to evaluate the zoonotic risk. We could not detect C. psittaci on either eggshells or eggshell membranes. However, C. psittaci isolates of different outer-membrane protein A (ompA) genotypes were cultured from the air of both turkey (genotypes A and C) and chicken (genotype D) hatching chambers. Zoonotic transmission occurred in all employees and a mixed infection with up to three different genotypes (A, D and C), also found in air samples, was discovered. Diagnostic monitoring and reporting of C. psittaci infections in poultry workers should be promoted. Additionally, an efficient veterinary vaccine and information campaigns on zoonotic risk and preventive measures against C. psittaci transmission would be beneficial to public health

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“…It was suggested that the electrostatic sampler introduced less impaction and desiccation stress than the BioStage impactor (Yao and Mainelis 2006c). In the meantime, filter sampling including gelatin filter was also investigated for collecting bioaerosols in numerous studies (Wang et al 2001;Burton et al 2005;Chen and Li 2005;Nehme et al 2008;Droogenbroeck et al 2009;Yao et al 2009a;Wu et al 2010). These studies suggested that the filter materials and the sampling stress might affect its overall performance.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Culturable Bacterial and Fungal Aerosol Diversity Obtained Using Different Samplers and Culturing Methods

Yao

2011

Aerosol Science and Technology

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In this study, biological collection efficiencies and culturable bacterial and fungal aerosol diversities were investigated when different bioaerosol sampling tools and culturing methods were applied. The samplers included Reuter centrifugal sampler (RCS) High Flow, BioSampler, electrostatic sampler, gelatin filter, BioStage impactor, mixed cellulose ester (MCE) filter as well as gravitational settling methods. For culturable bacterial aerosol diversity, the colony-forming units (CFUs) were washed off from the agar plates, and further went through polymerase chain reactionand denaturing gradient gel electrophoresis (PCR-DGGE). For culturable fungal aerosol diversity, microscopic identification method was applied.In general, the BioStage impactor, MCE filter, and the BioSampler remained robust when sampling culturable bioaerosols. The PCR-DGGE study revealed that the use of different samplers and culturing methods resulted in different culturable bioaerosol diversity. For indoor bacterial aerosols, the MCE filter with direct culturing on agar plates and the BioSampler resulted in the highest number of visible bands on DGGE gels, followed by the gravitational settling (26 • C), the RCS High Flow, electrostatic sampler, and the MCE filter extracted. However, for outdoor bacterial aerosols, the BioStage impactor obtained the highest number of visible bands followed by the BioSampler, MCE filter, electrostatic sampler, the RCS High Flow, and gravitational settling at 26• C. When sampling fungal aerosols, the electrostatic sampler was observed to produce the highest diversity. Similar to previous studies, Alternaria, Cladosporium, and Aspergillus species were found to dominate the fungal community in most samples collected here.The results obtained here suggested that the sampler design, sampling environments, and culturing methods should be considered together when assessing the biological aerosol exposure.

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Evaluation of bioaerosol sampling techniques for the detection of Chlamydophila psittaci in contaminated air

Cited by 35 publications

References 33 publications

Chlamydophila psittaci Zoonotic Risk Assessment in a Chicken and Turkey Slaughterhouse

Chlamydophila psittaci Zoonotic Risk Assessment in a Chicken and Turkey Slaughterhouse

Zoonotic transmission of Chlamydia psittaci in a chicken and turkey hatchery

Analysis of Culturable Bacterial and Fungal Aerosol Diversity Obtained Using Different Samplers and Culturing Methods

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