Evaluation of bio-chemical constituents in green gram associated with resistance to Callosobruchus maculatus during storage

Abstract

The pulse beetle, Callosobruchus maculatus (Fabricius) is an important limiting biotic factor in the storage of green gram. The present study was carried out to study the biochemical constituents imparting resistance/susceptibility to pulse beetle Callosobruchus maculatus Fabricius in green gram varieties under ambient conditions. The 9 promising varieties of green gram obtained from Oilseed Research Station, Jalgaon and evaluated for biochemical and physical parameters under storage conditions at Seed Testing Research Unit, MPKV, Rahuri. After storage period the seed were evaluated for biochemical parameters in which results showed that the protein content in seeds of different green gram varieties ranged from 20.23 to 22.32 percent. The highest per cent protein content was found in Kopargoan (22.32%). The less per cent protein content was observed in . The highest per cent carbohydrate content was found in Kopargoan (62.23 %), followed by PM-302-46 (61.82%). The less carbohydrate content was observed in . The grain infestation on number and weight basis ranged between 12.33 -21.40 and 78.36-85.14 per cent, respectively. The minimum grain infestation on number and weight basis was noticed in the variety BM-2003-1 (12.33 %) and (79.18 %) which was found statistically at par with BM-4 (15.33%) and (79.88%) and BM-2002-1(16.00 %) and (78.36 %). Under correlation between biochemical parameters and grain infestation by pulse beetle, it was observed that protein, carbohydrate, fat and moisture content indicated positively and non significant correlation with orientation of C. maculatus adults towards seeds of green gram.